Sulfitobacter porphyrae sp. nov., isolated from the red alga Porphyra yezoensis

Abstract

Gram-stain-negative, aerobic, halophilic bacteria, designated SCM-1 T , LCM10-1 and CTBL-B-147, were isolated from modified half-strength SWM-III medium, PES medium and thalli after laboratory cultivation of a red alga, Porphyra yezoensis. Phylogenetic analysis of 16S rRNA gene sequences indicated that the new isolates were affiliated to the genus Sulfitobacter of the class Alphaproteobacteria, and the 16S rRNA gene sequence similarity of the new isolates with the closest related species, Sulfitobacter mediterraneus CH-B427 The genus Sulfitobacter of the family Rhodobacteraceae in the class Alphaproteobacteria was described by Sorokin (1995) and currently includes nine species (http://www. bacterio.net/) that have been isolated from the H 2 S-O 2 interface of the Black Sea (Sorokin, 1995), a hyper-saline lake (Labrenz et al., 2000), starfish and sea grass (Ivanova et al., 2004). During the taxonomic investigation of the bacterial population in a laboratory-cultured red alga, Porphyra yezoensis, three bacteria designated SCM-1 T , LCM10-1 and CTBL-B-147 were isolated. In this study, we describe the polyphasic taxonomic study of the new isolates.Thalli of P. yezoensis U-51 were cultured in sterile, modified half-strength SWM-III medium (Ogata, 1970) at 17 u C for 5 weeks or in sterile PES medium (Provasoli, 1968) at 18 u C for 6 weeks. Samples of the modified halfstrength SWM-III medium, PES medium and thalli were plated on marine agar 2216 (MA; BD) and 10-fold diluted MA (1/10 MA), and then incubated at 20 u C for 2 weeks. Strain SCM-1 T was isolated on MA from the modified half-strength SWM-III medium, and strains LCM10-1 and CTBL-B-147 were isolated on 1/10 MA and MA from the thalli cultured in modified half-strength SWM-III Abbreviations: FAME, fatty acid methyl ester; ML, maximum-likelihood; MP, maximum-parsimony; NJ, neighbour-joining.

Sulfitobacter faviae sp. nov., isolated from the coral Favia veroni

“…Trees prepared with the neighbour-joining, maximum-likelihood and maximum-parsimony algorithms were found to be nearly identical although a few topological variations were recorded. This observation was in agreement with the data presented by different authors (Ivanova et al, 2004;Fukui et al, 2014).…”

“…The DNA G+C content varies between 55 and 63.7 mol%. Based on 16S rRNA gene sequence analysis, members of this genus are closely related to the monotypic genus Oceanibulbus (Wagner-Döbler et al, 2004) T in the phylogenetic tree (Ivanova et al, 2004;Kwak et al, 2014;Fukui et al, 2014).In the present study, we describe the phenotypic, genotypic and chemotaxonomic characteristics of three strains, i.e. S5-53 T , S6-62 and S6-64, isolated from mucus of the coral Favia veroni from the Andaman Sea, India.…”

“…The DNA G+C content varies between 55 and 63.7 mol%. Based on 16S rRNA gene sequence analysis, members of this genus are closely related to the monotypic genus Oceanibulbus (Wagner-Döbler et al, 2004) T in the phylogenetic tree (Ivanova et al, 2004;Kwak et al, 2014;Fukui et al, 2014).…”

“…The genus was introduced by Sorokin (1995) to accommodate two strains of heterotrophic bacteria isolated from the Black Sea with Sulfitobacter pontiacus as the type species. At the time of writing, the genus Sulfitobacter contains 15 recognized species (Parte, 2014) that were isolated from marine environments either from seawater (Sorokin, 1995;Kwak et al, 2014) or associated with marine invertebrates (Fukui et al, 2014;Hong et al, 2015) from different geographical regions and thought to play an important role in organic sulfur cycling in the ocean environment (Ivanova et al, 2004). Phenotypically, strains are Gram-negative, aerobic and rod-shaped.…”

“…The obtained nucleoside mixture was then separated by HPLC (Shimadzu) using an analytical column (Vydac 201 SP54, C18, 5 µm; 250Â4.6 mm) equipped with a guard column (201 GD54H; Vydac). The operating conditions were as follows: 35 C, 10 µl of sample, 0.3 M (NH 4 )H 2 PO 4 /acetonitrile, 40 : 1 (v/v), pH 4.4, as solvent and flow rate of 0.5 ml min T had a DNA G+C content of 61.0 mol%, a value close to that of Sulfitobacter porphyrae SCM-1 T (61.4 mol%, Fukui et al, 2014) and Sulfitobacter pseudonitzschiae H3 T (61.7 mol%, Hong et al, 2015) and well within the range of observed variability of G+C content for the genus Sulfitobacter, i.e. 55-63.7 mol% (Parte, 2014).…”