Contribution of Supercritical Fluid Chromatography coupled to High Resolution Mass Spectrometry and UV detections for the analysis of a complex vegetable oil – Application for characterization of a Kniphofia uvaria extract

Supercritical fluid chromatography for lipid analysis in foodstuffs

“…Parallel improvements in the hardware and chromatographic media have paved the way to a rebirth of the technique, into what can be defined as “ultrahigh performance supercritical fluid chromatography” (UHPSFC), as the remarkable reduction in void volumes and the higher pressure limits (1100 bar) have made SFC compatible with use of low id columns (sub‐2 μm) and core–shell particles. This in turn has led to dramatic improvements in the chromatographic efficiency and sensitivity, in a similar way as happened in the shift from HPLC to UHPLC . A very recent study was carried out by Ashraf‐Khorassani et al , employing state‐of‐the‐art SFC instrumentation with high precision for modified flow and backpressure regulation, in conjunction with different 1.8‐μm bonded silica particles.…”

“…All these studies and applications are reviewed in the literature, specifically for FFAs as well as for other lipid compounds . Open tubular capillary, packed capillary, and packed columns have been employed for SFC separation of FFAs, either as such, or in the form of FAMEs; some key applications to qualitative and quantitative food analysis are listed in Table . The use of open tubular columns allows gaining high efficiency at low pressure drop, and the selectivity can be easily tuned by using different stationary phases , while packed columns offer higher loadability and short analysis times.…”

“…In general, few studies have been published about the SFC–MS hyphenation using APCI interface, although this type of ionization may allow for direct introduction of the flow from the separation column and, thus, afford greater sensitivity. UHPSFC–APCI‐MS was performed by Duval et al , for the complete characterization of the lipid composition of an unknown vegetable oil. Besides FAs, also mono‐, di‐, and TAGs were separated, given the capability for ultrahigh pressure operation, onto seven columns connected in series, providing a total stationary phase length of >1 m.…”

Determination of fat‐ and water‐soluble vitamins by supercritical fluid chromatography: A review

“…CO 2 is characterized by easy‐to‐reach critical parameters of 31°C and 7.4 MPa but also low viscosity and high diffusivity. These parameters allow to apply higher flow rates and consequently shorten the analysis . For instance, the efficiency of the method may be improved when using stationary phase with higher number of theoretical plates as it was studied by Lesellier .…”

Theory and Practice of UHPLC and UHPLC–MS

“…Among them, three phases, diol, nonendcapped C18, and 2-ethylpyridine, were determined to be of particular interest (Grand-Guillaume Perrenoud et al, 2016). The analysis of both polar and nonpolar plant extracts gave evidence that UHPSFCeHRMS could be applied for the metabolite profiling of natural complex matrices (Duval et al, 2016;.…”