Contributions of functional genomics and proteomics to the study of immune responses in the Pacific white leg shrimp Litopenaeus vannamei

Robalino, Javier; Carnegie, Ryan B.; O’Leary, Nuala; Ouvry-Patat, Séverine A.; Vega, Enrique de la; Prior, Sarah; Gross, Paul S.; Browdy, Craig L.; Chapman, R. W.; Schey, Kevin L.; Warr, Gregory W.

doi:10.1016/j.vetimm.2008.10.329

Cited by 45 publications

(26 citation statements)

References 64 publications

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“…The data in present work was obtained by proteomic approach, while some transcriptomic analyses on shrimp response to WSSV infection have been reported in recent literatures [11], [43], [44]. Our results have some accordance with the findings obtained from these literatures.…”

Section: Discussionsupporting

confidence: 91%

Proteomic Analysis of Differentially Expressed Proteins in Fenneropenaeus chinensis Hemocytes upon White Spot Syndrome Virus Infection

Tang

Xing

et al. 2014

PLoS ONE

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To elucidate molecular responses of shrimp hemocytes to white spot syndrome virus (WSSV) infection, two-dimensional gel electrophoresis was applied to investigate differentially expressed proteins in hemocytes of Chinese shrimp (Fenneropenaeus chinensis) at 24 h post infection (hpi). Approximately 580 protein spots were detected in hemocytes of healthy and WSSV-infected shrimps. Quantitative intensity analysis revealed 26 protein spots were significantly up-regulated, and 19 spots were significantly down-regulated. By mass spectrometry, small ubiquitin-like modifier (SUMO) 1, cytosolic MnSOD, triosephosphate isomerase, tubulin alpha-1 chain, microtubule-actin cross-linking factor 1, nuclear receptor E75 protein, vacuolar ATP synthase subunit B L form, inositol 1,4,5-trisphosphate receptor, arginine kinase, etc., amounting to 33 differentially modulated proteins were identified successfully. According to Gene Ontology annotation, the identified proteins were classified into nine categories, consisting of immune related proteins, stimulus response proteins, proteins involved in glucose metabolic process, cytoskeleton proteins, DNA or protein binding proteins, proteins involved in steroid hormone mediated signal pathway, ATP synthases, proteins involved in transmembrane transport and ungrouped proteins. Meanwhile, the expression profiles of three up-regulated proteins (SUMO, heat shock protein 70, and arginine kinase) and one down-regulated protein (prophenoloxidase) were further analyzed by real-time RT-PCR at the transcription level after WSSV infection. The results showed that SUMO and heat shock protein 70 were significantly up-regulated at each sampling time point, while arginine kinase was significantly up-regulated at 12 and 24 hpi. In contrast, prophenoloxidase was significantly down-regulated at each sampling time point. The results of this work provided preliminary data on proteins in shrimp hemocytes involved in WSSV infection.

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Section: Discussionsupporting

confidence: 91%

Proteomic Analysis of Differentially Expressed Proteins in Fenneropenaeus chinensis Hemocytes upon White Spot Syndrome Virus Infection

Tang

Xing

et al. 2014

PLoS ONE

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“…A genome sequencing project for the first decapod crustacean, the commercially most valuable Pacific white shrimp Litopenaeus vannamei, is on the way and the necessary EST databases, linkage maps or BAC libraries are rapidly increasing (Stillman et al 2008;Koyama et al 2010). Moreover, techniques for the identification of genes and their products are more and more adopted for shrimp and crayfish (Shechter et al 2007;Robalino et al 2009). For the short term, the gap of knowledge on genetics of ageing in decapod crustaceans could be bridged by searching for ageing genes on the basis of Drosophila data (Helfand and Rogina 2003;Kennedy 2008;Paaby and Schmidt 2009).…”

Section: Suitability Of the Marbled Crayfish For Research On Genetic mentioning

confidence: 99%

Suitability of the clonal marbled crayfish for biogerontological research: a review and perspective, with remarks on some further crustaceans

Vogt

2010

Biogerontology

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This article examines the suitability of the parthenogenetic marbled crayfish for research on ageing and longevity. The marbled crayfish is an emerging laboratory model for development, epigenetics and toxicology that produces up to 400 genetically identical siblings per batch. It is easily cultured, has an adult size of 4-9 cm, a generation time of 6-7 months and a life span of 2-3 years. Experimental data and biological peculiarities like isogenicity, direct development, indeterminate growth, high regeneration capacity and negligible senescence suggest that the marbled crayfish is particularly suitable to investigate the dependency of ageing and longevity from non-genetic factors such as stochastic developmental variation, allocation of metabolic resources, damage and repair, caloric restriction and social stress. It is also well applicable to examine alterations of the epigenetic code with increasing age and to identify mechanisms that keep stem cells active until old age. As a representative of the sparsely investigated crustaceans and of animals with indeterminate growth and extended brood care the marbled crayfish may even contribute to evolutionary theories of ageing and longevity. Some relatives are recommended as substitutes for investigation of topics, for which the marbled crayfish is less suitable like genetics of ageing and achievement of life spans of decades under conditions of low food and low temperature. Research on ageing in the marbled crayfish and its relatives is of practical relevance for crustacean fisheries and aquaculture and may offer starting points for the development of novel anti-ageing interventions in humans.

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“…AK had increased expression in Jasus edwardsii and P. stylirostris both infected with WSSV (Speed et al 2001;Astrofsky et al 2002). Similar increased expression of AK was also observed in S. serrata infected with that virus, indicating that AK regulates physiological response during viral infection, and that it is associated to the mechanism of innate immunity (Robalino et al 2009), this could explain low metabolism observed in some species infected with WSSV. It is interesting that among the identified proteins, two AKs have different pIs and molecular weight, suggesting that there are different AKs in the hemolymph of S. serrata and they should play different roles in cellular response to viral infection (Liu et al 2011).…”

Section: Discussionmentioning

confidence: 53%

“…Thus, knowing the probable infection, biosafety measures can be adopted early and applied for future development of antiviral defenses, because the animal needs a fast response in the beginning of infection to combat the virus with efficacy and minimize the impacts of infection in shrimp farming. In recent years, studies on shrimp immunity at the molecular level has increased rapidly for both cellular and humoral responses, in order to elucidate host immune response mechanisms to viral infection (Bourchookarn et al 2008;Robalino et al 2009;Chai et al 2010;Zhang et al 2010;Somboonwiwat et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…In this context, the study of crustacean immune system is emerging as a promising strategy, since it allows better understanding about the susceptibility and resistance of animals to pathogens and parasites and provides valuable insights into the establishment of health indicators and immunomarkers for genetic selection of animals resistant to infection (Barracco et al 2007;Robalino et al 2009). …”

Section: Introductionmentioning

confidence: 99%

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Differential expression of proteins in the gills of Litopenaeus vannamei infected with white spot syndrome virus

2014

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Determination of differentially expressed protein profile is necessary to understand the host response to viral infection. Proteomics can be applied as a tool to examine white shrimp Litopenaeus vannamei molecular responses against white spot syndrome virus (WSSV) infection, thus enabling development of effective strategies to reduce their impact on farms. In the present study, specific pathogen-free shrimp was tested against WSSV infection under several time intervals. Shrimps were submitted to a viral load of with 5.5 9 10 6 viral copies in 100 lL/shrimp. The monitoring of infection was performed in intervals of 6, 12, 24, 48 and 72 h after infection. The analysis was realized using 2-DE, and differentially expressed proteins were identified by MALDI-TOF mass spectrometry (MS) peptide mass fingerprint (PMF). Between the differentially expressed proteins found in the infected animals, the most important were identified as caspase-2, ubiquitin and F1-ATP synthase. They are interesting candidates for biomarkers because could be related to the beginning of apoptosis process. The differentially expressed protein profile creates a new paradigm in the analysis of L. vannamei shrimp molecular response to WSSV infection and in virus-host relationship. Furthermore, it proposes potential biomarkers that allow strategies both selecting less susceptible individuals and reducing the impact of viruses on farms.

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Contributions of functional genomics and proteomics to the study of immune responses in the Pacific white leg shrimp Litopenaeus vannamei

Cited by 45 publications

References 64 publications

Proteomic Analysis of Differentially Expressed Proteins in Fenneropenaeus chinensis Hemocytes upon White Spot Syndrome Virus Infection

Proteomic Analysis of Differentially Expressed Proteins in Fenneropenaeus chinensis Hemocytes upon White Spot Syndrome Virus Infection

Suitability of the clonal marbled crayfish for biogerontological research: a review and perspective, with remarks on some further crustaceans

Differential expression of proteins in the gills of Litopenaeus vannamei infected with white spot syndrome virus

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