Control of bovine virus diarrhoea at the herd level: Reducing the risk of false negatives in the detection of persistently infected cattle

Laureyns, Jozef; Ribbens, Stefaan; Kruif, Aart de

doi:10.1016/j.tvjl.2008.11.014

Cited by 38 publications

(39 citation statements)

References 46 publications

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“…The loads of virus shed by the PIs into the environment are so high that even short contact with such animals may lead to infection in the herd (20). BVDV persistent infection is observed in 1%-2% of cattle population (13,18,19,24). In Poland, prevalence of PI animals is similar to other geographic regions of Europe.…”

Section: Discussionmentioning

confidence: 98%

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Evaluation of diagnostic methods to distinguish between calves persistently and transiently infected with bovine viral diarrhoea virus in respect to the presence of maternal antibodies

Larska

Kuta

Polak

2013

Bulletin of the Veterinary Institute in Pulawy

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Two issues concerning virus detection and identification of persistently infected (PI) cattle were analysed in the study: 1) interference by maternal antibodies and 2) discrimination between PI and transiently infected (TI) animals. Antigen ELISA and RT-PCR based methods were compared using serum samples from natural and experimental PI and TI calves. RT-PCR and realtime RT-PCR using primers within 5'UTR region were more sensitive in detecting PI animals than E rns and NS3 antigen capture

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Section: Discussionmentioning

confidence: 98%

“…The presence of colostral antibodies in serum samples from young animals (Fig. 1B) can interfere with the antigen capture ELISA and may lead to false negative results (4,11,18,28). Ear-notch samples instead of serum have been proposed as samples for the antigen capture ELISA to avoid false negative results in seropositive PI animals (8).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of diagnostic methods to distinguish between calves persistently and transiently infected with bovine viral diarrhoea virus in respect to the presence of maternal antibodies

Larska

Kuta

Polak

2013

Bulletin of the Veterinary Institute in Pulawy

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“…This requires the design of disease prevention protocols ideally including breeding, animal purchase and stock replacement, as well as herd and operator hygiene policies (Laureyns et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of bovine viral diarrhoea virus in Hungary — situation before launching an eradication campaign

Kővágó

Forgách

Szabára

et al. 2015

Acta Veterinaria Hungarica

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Bovine viral diarrhoea (BVD) is a viral disease appearing in various forms and causing high economic losses in the cattle stocks of Hungary. The aim of the present study was to determine the prevalence of bovine viral diarrhoea virus (BVDV) in Hungary through a monitoring survey carried out on samples collected in cattle-keeping units throughout the country. Since no such survey had been carried out in Hungary during the last thirty years, our study may serve as a basis for later monitoring investigations aimed at following the progress of an expected eradication campaign of BVD. The tests were carried out using an ELISA method, on a total of 1200 blood samples submitted from 54 cattle herds. The herds had not been vaccinated against BVDV before the sampling. Out of the 1200 samples, 521 proved to be positive (43.4%), 40 gave doubtful result (3.3%) and 639 were negative (53.3%). In some stocks the samples were collected from cows having completed several lactation periods, and therefore the seronegativity indicates the BVDV-free status of the given stock. Moreover, among the positive herds we found a few where the seropositivity rate was rather low (< 5%). According to the results of the survey, a rather high portion (about one third) of the cattle-keeping units of Hungary can be regarded as BVDV free, which ratio is much higher than had been expected on the basis of surveys carried out on a lower number of samples and in smaller regions of the country. Hence, the chances of an eradication campaign launched in the near future, or carried out parallel to the IBR eradication programme, are better than previously expected.

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“…There is a proportional relationship of the maternal antibody titers with the efficacy of the isolation of the BVDV and the direct enzyme immunoassay, i.e., the higher the mean titer of the maternal antibodies, the lower will be the possibility of virus isolation and the detection of the viral antigens by the enzyme immunoassay, thus indicating the inefficacy of these two techniques in the diagnosis of the PI animals following the ingestion of the colostrum. Laureyns et al (2010) indicated the use of the direct enzyme immunoassay assay effective from 10 weeks after birth because of the decrease in maternal antibody levels in the blood serum of calves by the end of this period. Moreover, Bruschke et al (1998) demonstrated that maternal antibodies of BVDV in the serum of 30-week-old PI calves.…”

Section: Rt-pcrmentioning

confidence: 99%

“…The indirect immunoenzymatic assays are indicated from the 10 th week post birth, when a significant decrease in the colostral antibodies can be observed (LAUREYNS et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Detection of Bovine Viral Diarrhea virus infection in newborn calves before colostrum intake

Martin

Baccili

Silva

et al. 2016

SCA

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The detection of Bovine Viral Diarrhea virus (BVDV) infection, especially among persistently infected calves (PI), should be performed earlier in order to eliminate the source of the infection and to prevent the spread of the disease in the herd. However, colostrum intake can influence the results of some of the tests used to diagnose the BVDV infection. Therefore, this study evaluated the efficacy of serum neutralization (SN) test in conjunction with reverse transcription-polymerase chain reaction (RT-PCR) for the diagnosis of BVDV infection before colostrum intake. The deliveries of the animals were assisted to select 52 newborn Holstein calves for inclusion in the study. Initially, the whole blood and serum samples were collected from the calves before (T0) and after (T1) the colostrum intake. The calves that were RT-PCR positive at any of the time-points were retested on the 30 th day post birth (T2). The presence of specific antibodies for BVDV was evaluated by SN, and that of viral RNA by the RT-PCR. The BVDV-specific antibodies were observed in the serum of 13.46% (7/52) of the calves at T0 because of fetal infection. At T1, seroconversion was observed in 100% (52/52) of the calves. The geometric mean titers (GMT) of the antibodies for BVDV increased significantly from T0 (14.52) to T1 (2490) (P = 0.0001). Of the four calves that were RT-PCR positive before colostrum intake (T0), two were seronegative and two, seropositive. Of the forty-eight RT-PCR negative calves, five were seropositive. After 30 days post birth, all of the animals tested negative by RT-PCR, thus excluding the possibility of persistent infection. The association observed between the results of the SN and RT-PCR assays at T0 (P = 0.025) could not be observed at T1 (P > 0.05). The SN test before the colostrum intake allowed the detection of fetal infection in the herd; however, this test was ineffective as a diagnostic method after the transfer of passive immunity. The confirmation of the results of the SN assay by those of the RT-PCR was essential for the identification of the infected calves before colostrum intake. Key words: Bovine viral diarrhea. Passive immunity. Persistent infection. Polymerase chain reaction. Serum neutralization. ResumoInfecção causada pelo BVDV, especialmente bezerras persistentemente infectadas (PI), deve ser detectada precocemente para eliminação da fonte de infecção e disseminação da doença no rebanho. No entanto, a mamada de colostro interfere em alguns testes adotados para o diagnóstico da Diarréia Viral Bovina. Assim, esta pesquisa avaliou o uso da soroneutralização (SN) em associação com a reação em cadeia de polimerase precedida da transcrição reversa (RT-PCR) para diagnóstico da infecção pelo BVDV antes da mamada de colostro. Partos foram acompanhados para seleção de 52 bezerras da raça Holandesa. Inicialmente foram coletadas amostras de sangue total e soro de todos os animais antes (T0) e após a mamada do colostro (T1). Os animais positivos no RT-PCR em qualquer momento foram retestados aos 30 ...

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Control of bovine virus diarrhoea at the herd level: Reducing the risk of false negatives in the detection of persistently infected cattle

Cited by 38 publications

References 46 publications

Evaluation of diagnostic methods to distinguish between calves persistently and transiently infected with bovine viral diarrhoea virus in respect to the presence of maternal antibodies

Evaluation of diagnostic methods to distinguish between calves persistently and transiently infected with bovine viral diarrhoea virus in respect to the presence of maternal antibodies

Seroprevalence of bovine viral diarrhoea virus in Hungary — situation before launching an eradication campaign

Detection of Bovine Viral Diarrhea virus infection in newborn calves before colostrum intake

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