Control of postharvest grey mould decay of nectarine by tea polyphenol combined with tea saponin

Abstract

Significance and Impact of the Study: This study demonstrates that the combination of TP and TS has exhibited synergistic antifungal interactions against Botrytis cinerea, and it suggests that their combination may be useful and effective agents for the control of nectarine grey mould decay. Such natural products therefore represent a promising alternative to synthetic fungicides in the control of nectarine postharvest diseases. AbstractThe control efficacy of tea polyphenol (TP) in combination with tea saponin (TS) against nectarine grey mould decay caused by Botrytis cinerea and the underlying mechanism were investigated. The in vitro experiments showed that both TP and TS inhibited the mycelial growth in a dose-dependent manner, and their combinations exhibited synergistic antifungal interactions with the synergistic ratios (SR) exceeding 1Á5. The in vivo experiments showed that disease incidence and lesion diameter of grey mould of inoculated fruit were significantly lowered after being treated with the combination of TP and TS; furthermore, the activities of phenylalanine ammonia-lyase (PAL), peroxidase (POD), polyphenol oxidase (PPO), chitinase and b-1,3-glucanase of inoculated fruit as well as the contents of total phenolic and lignin were significantly induced, the respiration rate of inoculated fruit was significantly decreased and therefore the quality decrease was accordingly retarded. These results revealed that TP in combination with TS could control grey mould of inoculated nectarines and their mechanism of action might be attributed to their active components, the induction of defensive system and the regulation of respiration.

In vitro Antifungal Activity and Mechanism of Action of Tea Polyphenols and Tea Saponin against <b><i>Rhizopus stolonifer</i></b>

“…Both TP and TS have good antifungal activity and can inhibit the growth of some postharvest pathogens of fruit Hao et al, 2010;Liu et al, 2010a;Liu et al, 2010b;Yang et al, 2013]. However, to our knowledge, no study has reported on the antifungal activity of TP and TS against R. stolonifer , and the antifungal mechanism is not fully understood.…”

Tea polyphenol is a potential antifungal agent for the control of obligate biotrophic fungus in plants

“…To our knowledge, related in vivo studies were conducted only on M. fructicola and Bo. cinerea , which experiments showed that the activities of phenylalanine ammonia‐lyase, peroxidase, polyphenol oxidase, chitinase and β‐1,3‐glucanase of inoculated fruit as well as the contents of total phenolic and lignin were significantly induced (Chen et al., ; Yang, Jiang, et al., ).…”

“…TP components, particularly catechins, can be divided into four categories according to their chemical structures: epigallocatechin gallate, epigallocatechin, epicatechin gallate and epicatechin (Chai, Wang, & Zhang, ). TP has been currently reported to possess antimicrobial properties against a wide spectrum of animal and plant microbes (Afzal, Safer, & Menon, ; Taylor, Hamilton‐Miller, & Stapleton, ), particularly plant fungal pathogens, such as Bipolaria maydis , Calletotrichum musae , Fusarium oxysporum , Botrytis cinerea and Diplodia natalensis (Liu, Guo, Cheng, Liu, Long, & Deng, ; Liu, Guo, Liu, Cheng, Wang, Long, & Deng, ; Wang et al., ; Yang, Jiang, Chen, & Zhang, ). To our knowledge, however, only few studies have focused on the in vivo antimicrobial activity of TP, particularly on plant obligate biotrophic pathogens.…”

Antifungal activity and mechanism of tea polyphenols against Rhizopus stolonifer

“…The homogenate was centrifuged at 10,0009g for 20 min at 4°C, and the supernatant was collected for enzyme assay. The activities of PAL, PPO, POD, GLU, and CHI were assayed as described by Yang et al (2013). PAL, PPO, and POD activities were expressed as U, where 1 U was respectively defined as change in A 290 nm (PAL), A 420 nm (PPO) and A 470 nm (POD)/ min/g fresh weight (FW) of pulp.…”