2021
DOI: 10.1039/d1sc04342k
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Controlled movement of ssDNA conjugated peptide through Mycobacterium smegmatis porin A (MspA) nanopore by a helicase motor for peptide sequencing application

Abstract: A new technique for single molecular peptide sequencing is demonstrated by translocation of ssDNA-conjugated-peptide through MspA nanopore which is regulated by a DNA helicase motor.

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Cited by 52 publications
(34 citation statements)
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“…This was similarly demonstrated by Chen et al. suggesting that stretching the analyte, thus limiting thermal motion would improve the resolution ( Chen et al., 2021 ). Yan et al.…”
Section: Sensing Biomedical Relevant Molecules With Biological Nanoporesmentioning
confidence: 57%
“…This was similarly demonstrated by Chen et al. suggesting that stretching the analyte, thus limiting thermal motion would improve the resolution ( Chen et al., 2021 ). Yan et al.…”
Section: Sensing Biomedical Relevant Molecules With Biological Nanoporesmentioning
confidence: 57%
“…8 The other strategy employs DNA motor enzymes to ratchet a peptide–oligonucleotide conjugate into the nanopore. 6,7,9 The former method requires the digestion of the protein. Then, all peptide fragments should be captured and recognized by a nanopore.…”
Section: Introductionmentioning
confidence: 99%
“…One of the major concerns of OmpF sensing peptides is the short residence time; many approaches can potentially be utilized to boost the peptide sensing capability through nanopores, e.g. forming peptide-DNA conjugation for enzymedirected amino acid stepwise scanning (10,24,25). Moreover, we found strong indications that the chirality of the polar sidechain from serine (Mol-13) creates additional interactions with the residues in the CZ of OmpF (e.g.…”
mentioning
confidence: 80%