Controlled Multicenter Evaluation of a Bacteriophage-Based Method for Rapid Detection of Staphylococcus aureus in Positive Blood Cultures

Bhowmick, Tanaya; Mirrett, Stanley; Reller, L. Barth; Price, Connie S.; Qi, Chao; Weinstein, Melvin P.; Kirn, T.

doi:10.1128/jcm.02967-12

Cited by 41 publications

(24 citation statements)

References 37 publications

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“…It is noteworthy that both recent studies that evaluated a commercially available phage amplification assay for S. aureus detection and determination of methicillin susceptibility directly from positive blood cultures demonstrated the excellent specificity but moderate sensitivity of this approach (31,32). This is quite contrary to the results obtained with the test used here, since we observed perfect sensitivity but moderate specificity of the phagebased test.…”

Section: Discussioncontrasting

confidence: 57%

“…Recently, novel bacteriophage-based diagnostic applications have appeared, as reviewed elsewhere (22,30). They include susceptibility testing with phage amplification assays to detect multidrugresistant pathogens (31)(32)(33). Tailspike proteins, which are used by many phages to bind specifically to the cell surface, have recently been suggested for use as molecular probes for bacterial detection (34).…”

Section: Discussionmentioning

confidence: 99%

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Bacteriophage-Based Latex Agglutination Test for Rapid Identification of Staphylococcus aureus

Idelevich

Walther²,

Molinaro³

et al. 2014

J Clin Microbiol

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e Rapid diagnosis is essential for the management of Staphylococcus aureus infections. A host recognition protein from S. aureus bacteriophage phiSLT was recombinantly produced and used to coat streptavidin latex beads to develop a latex agglutination test (LAT). The diagnostic accuracy of this bacteriophage-based test was compared with that of a conventional LAT, Pastorex StaphPlus, by investigating a clinical collection of 86 S. aureus isolates and 128 coagulase-negative staphylococci (CoNS) from deep tissue infections. All of the clinical S. aureus isolates were correctly identified by the bacteriophage-based test. While most of the CoNS were correctly identified as non-S. aureus isolates, 7.9% of the CoNS caused agglutination. Thus, the sensitivity of the bacteriophage-based LAT for identification of S. aureus among clinical isolates was 100%, its specificity was 92.1%, its positive predictive value (PPV) was 89.6%, and its negative predictive value (NPV) was 100%. The sensitivity, specificity, PPV, and NPV of the Pastorex LAT for the identification of S. aureus were 100%, 99.2%, 98.9%, and 100%, respectively. Among the additionally tested 35 S. aureus and 91 non-S. aureus staphylococcal reference and type strains, 1 isolate was false negative by each system; 13 and 8 isolates were false positive by the bacteriophage-based and Pastorex LATs, respectively. The ability of the phiSLT protein to detect S. aureus was dependent on the presence of wall teichoic acid (WTA) and corresponded to the production of ribitol phosphate WTA, which is found in most S. aureus clones but only a small minority of CoNS. Bacteriophage-based LAT identification is a promising strategy for rapid pathogen identification. Finding more specific bacteriophage proteins would increase the specificity of this novel diagnostic approach.

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Section: Discussioncontrasting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Bacteriophage-Based Latex Agglutination Test for Rapid Identification of Staphylococcus aureus

Idelevich

Walther²,

Molinaro³

et al. 2014

J Clin Microbiol

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“…The speed of production of bacteriophage progeny in a resistant organism, therefore, allows for earlier detection of a resistant bacterium. Advances in phage technology in multidrug-resistant tuberculosis 16 are now being applied across a variety of surgically relevant resistant organisms, including the KeyPath assay (MicroPhage, Longmont, CO), 17,18 in multidrugresistant S aureus infections. Incubation with cefoxitin (a methicillin surrogate) provides further information about the presence of MRSA.…”

Section: Bacteriophagesmentioning

confidence: 99%

Advancing Technologies for the Diagnosis and Management of Infections

Heffernan

Fox

2014

Surgical Clinics of North America

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“…The test also discriminates between MRSA and MSSA using Cefoxitin in the assay; MRSA grow in the presence of cefoxitin, resulting in signal emission, in contrast to MSSA. A recent evaluation study reported 91.8% sensitivity and 98.3% specificity for detection of S. aureus …”

Section: Emerging Technologies For Bacterial Identification and Antibmentioning

confidence: 99%

Identification and Antibiotic‐Susceptibility Profiling of Infectious Bacterial Agents: A Review of Current and Future Trends

Maugeri

Lychko

Sobral

et al. 2018

Biotechnology Journal

131

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Antimicrobial resistance is one of the most worrying threats to humankind with extremely high healthcare costs associated. The current technologies used in clinical microbiology to identify the bacterial agent and profile antimicrobial susceptibility are time-consuming and frequently expensive. As a result, physicians prescribe empirical antimicrobial therapies. This scenario is often the cause of therapeutic failures, causing higher mortality rates and healthcare costs, as well as the emergence and spread of antibiotic resistant bacteria. As such, new technologies for rapid identification of the pathogen and antimicrobial susceptibility testing are needed. This review summarizes the current technologies, and the promising emerging and future alternatives for the identification and profiling of antimicrobial resistance bacterial agents, which are expected to revolutionize the field of clinical diagnostics.

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Controlled Multicenter Evaluation of a Bacteriophage-Based Method for Rapid Detection of Staphylococcus aureus in Positive Blood Cultures

Cited by 41 publications

References 37 publications

Bacteriophage-Based Latex Agglutination Test for Rapid Identification of Staphylococcus aureus

Bacteriophage-Based Latex Agglutination Test for Rapid Identification of Staphylococcus aureus

Advancing Technologies for the Diagnosis and Management of Infections

Identification and Antibiotic‐Susceptibility Profiling of Infectious Bacterial Agents: A Review of Current and Future Trends

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