Controlled release of ciliary neurotrophic factor from bioactive nerve grafts promotes nerve regeneration in rats with facial nerve injuries

Ma, Fukai; Wang, Hanming; Yang, Xiaosheng; Wu, Yiwei; Liao, Chenlong; Xie, Bingran; Li, Yang; Zhang, Wenchuan

doi:10.1002/jbm.a.37327

Cited by 5 publications

(3 citation statements)

References 50 publications

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“…To improve regeneration in an AN graft, researchers started to add neurotrophic factors such as NGF, BDNF, and ciliary neurotrophic factor or cells starting approximately 2 decades ago. 8,[20][21][22]24 Candidate cells have included SCs 1,2,7 and adipose-derived stem cells, 23 but clinical application has not been possible because of problems associated with lengthy cultivation time in vitro and risk of infection. Another candidate is induced pluripotent stem cells, 25,26 which are harvested from the patients themselves and cultivated in vitro.…”

Section: Discussionmentioning

confidence: 99%

Potential of an artificial nerve graft containing Schwann cells for the treatment of a 20-mm nerve defect in rats

Suzuki

Ichihara

Hayashi

et al. 2023

Journal of Neurosurgery

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OBJECTIVE In a previous study, the authors showed that the migration of Schwann cells (SCs) through end-to-side (ETS) neurorrhaphy promotes axonal regrowth within an acellular nerve graft. In the present study, the authors investigated whether a similar strategy using an artificial nerve (AN) would allow reconstruction of a long nerve gap (20 mm) in rats. METHODS Forty-eight 8- to 12-week-old Sprague Dawley rats were divided into control (AN) and experimental (SC migration-induced AN [SCiAN]) groups. Prior to the experiment, the ANs used in the SCiAN group were populated in vivo with SCs over a 4-week period by ETS neurorrhaphy onto the sciatic nerve. In both groups, a 20-mm sciatic nerve defect was reconstructed in an end-to-end fashion using 20-mm ANs. Sections from the nerve graft and distal sciatic nerve in both groups underwent assessments at 4 weeks for SC migration by immunohistochemical analysis and quantitative reverse transcription–polymerase chain reaction. At 16 weeks, axonal elongation was assessed by immunohistochemical analysis, histomorphometry, and electron microscopy. The number of myelinated fibers was counted, the g-ratio was calculated, and myelin sheath thickness and axon diameter were measured. Furthermore, functional recovery was evaluated at 16 weeks using the Von Frey filament test for sensory recovery and by calculating the muscle fiber area for motor recovery. RESULTS The area occupied by SCs at 4 weeks and by axons at 16 weeks was significantly larger in the SCiAN group than in the AN group. Histomorphometric evaluation of the distal sciatic nerve revealed a significantly greater number of axons. At 16 weeks, plantar perception in the SCiAN group was significantly better, demonstrating improvement in sensory function. However, no tibialis anterior muscle motor improvement was observed in either group. CONCLUSIONS The induction of SC migration into an AN by ETS neurorrhaphy is a useful technique for repairing 20-mm nerve defects in rats, with better nerve regeneration and sensory recovery. No motor recovery was observed in either group; however, motor recovery might require a longer period of time than the lifespan of the AN used in this study. Future studies should investigate whether structural and material reinforcement of the AN, to lower its decomposition rate, can improve functional recovery.

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Section: Discussionmentioning

confidence: 99%

Potential of an artificial nerve graft containing Schwann cells for the treatment of a 20-mm nerve defect in rats

Suzuki

Ichihara

Hayashi

et al. 2023

Journal of Neurosurgery

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“…Collagen has been widely studied and reported since it was first used as a facial nerve conduit in 1998 [57,58]. Now, researchers have synthesized biodegradable and bioactive collagen nerve grafts [59] to sustainably release neural factors. When used to repair facial nerve defects, collagen conduit can offer a safe regeneration environment and repair space for nerve regeneration.…”

Section: Collagenmentioning

confidence: 99%

Porous Organic Materials in Tissue Engineering: Recent Advances and Applications for Severed Facial Nerve Injury Repair

Sun,

Cao,

Pan

et al. 2024

Molecules

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The prevalence of facial nerve injury is substantial, and the restoration of its structure and function remains a significant challenge. Autologous nerve transplantation is a common treatment for severed facial nerve injury; however, it has great limitations. Therefore, there is an urgent need for clinical repair methods that can rival it. Tissue engineering nerve conduits are usually composed of scaffolds, cells and neurofactors. Tissue engineering is regarded as a promising method for facial nerve regeneration. Among different factors, the porous nerve conduit made of organic materials, which has high porosity and biocompatibility, plays an indispensable role. This review introduces facial nerve injury and the existing treatment methods and discusses the necessity of the application of porous nerve conduit. We focus on the application of porous organic polymer materials from production technology and material classification and summarize the necessity and research progress of these in repairing severed facial nerve injury, which is relatively rare in the existing articles. This review provides a theoretical basis for further research into and clinical interventions on facial nerve injury and has certain guiding significance for the development of new materials.

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“…CNTF induces neuronal survival and differentiation. It has neuroprotective, promyelinating, anti-apoptotic properties [ 111 , 182 ]. It has beneficial effects in neurodegenerative diseases.…”

Section: Beneficial Properties Of Sodium Benzoatementioning

confidence: 99%

Sodium Benzoate—Harmfulness and Potential Use in Therapies for Disorders Related to the Nervous System: A Review

Walczak-Nowicka

Herbet

2022

Nutrients

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Currently, due to the large number of reports regarding the harmfulness of food additives, more and more consumers follow the so-called “clean label” trend, i.e., prefer and choose the least-processed food products. One of the compounds known as a preservative with a high safety profile is sodium benzoate. While some studies show that it can be used to treat conditions such as depression, pain, schizophrenia, autism spectrum disorders, and neurodegenerative diseases, others report its harmfulness. For example, it was found to cause mutagenic effects, generate oxidative stress, disrupt hormones, and reduce fertility. Due to such disparate results, the purpose of this study is to comprehensively discuss the safety profile of sodium benzoate and its potential use in neurodegenerative diseases, especially in autism spectrum disorder (ASD), schizophrenia, major depressive disorder (MDD), and pain relief.

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Controlled release of ciliary neurotrophic factor from bioactive nerve grafts promotes nerve regeneration in rats with facial nerve injuries

Cited by 5 publications

References 50 publications

Potential of an artificial nerve graft containing Schwann cells for the treatment of a 20-mm nerve defect in rats

Potential of an artificial nerve graft containing Schwann cells for the treatment of a 20-mm nerve defect in rats

Porous Organic Materials in Tissue Engineering: Recent Advances and Applications for Severed Facial Nerve Injury Repair

Sodium Benzoate—Harmfulness and Potential Use in Therapies for Disorders Related to the Nervous System: A Review

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