Controlled Release of Naringin in GelMA-Incorporated Rutile Nanorod Films to Regulate Osteogenic Differentiation of Mesenchymal Stem Cells

Shao, Yangjie; You, Dongqi; Lou, Yiting; Li, Jianhua; Ying, Binbin; Cheng, Kui; Weng, Wenjian; Wang, Huiming; Yu, Mengfei; Dong, Lingqing

doi:10.1021/acsomega.9b02751

Cited by 26 publications

(19 citation statements)

References 32 publications

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“…At 72 h, less than 10% of naringin remained in GelMA-N compared to more than 45% in nanocomposite hydrogels. The obtained release profiles of GelMA and nanocomposite GelMA are comparable to those obtained in the literature for naringin and other therapeutic molecules [ 42 , 60 , 61 ]. This suggests that GelMA scaffolds can be successfully loaded with LipoN and provide a controlled extended release of the encapsulated flavonoid, which might improve its in vivo bioavailability and hence its therapeutic effect.…”

Section: Resultssupporting

confidence: 82%

Gelatin Methacryloyl (GelMA) Nanocomposite Hydrogels Embedding Bioactive Naringin Liposomes

et al. 2020

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The development of nanocomposite hydrogels that take advantage of hierarchic building blocks is gaining increased attention due to their added functionality and numerous biomedical applications. Gathering on the unique properties of these platforms, herein we report the synthesis of bioactive nanocomposite hydrogels comprising naringin-loaded salmon-derived lecithin nanosized liposomal building blocks and gelatin methacryloyl (GelMA) macro-sized hydrogels for their embedding. This platform takes advantage of liposomes’ significant drug loading capacity and their role in hydrogel network reinforcement, as well as of the injectability and light-mediated crosslinking of bioderived gelatin-based biomaterials. First, the physicochemical properties, as well as the encapsulation efficiency, release profile, and cytotoxicity of naringin-loaded nanoliposomes (LipoN) were characterized. Then, the effect of embedding LipoN in the GelMA matrix were characterized by studying the release behavior, swelling ratio, and hydrophilic character, as well as the rheological and mechanical properties of GelMA and GelMA-LipoN functionalized hydrogels. Finally, the dispersion of nanoliposomes encapsulating a model fluorescent probe in the GelMA matrix was visualized. The formulation of naringin-loaded liposomes via an optimized procedure yielded nanosized (114 nm) negatively charged particles with a high encapsulation efficiency (~99%). Naringin-loaded nanoliposomes administration to human adipose-derived stem cells confirmed their suitable cytocompatibility. Moreover, in addition to significantly extending the release of naringin from the hydrogel, the nanoliposomes inclusion in the GelMA matrix significantly increased its elastic and compressive moduli and decreased its swelling ratio, while showing an excellent dispersion in the hydrogel network. Overall, salmon-derived nanoliposomes enabled the inclusion and controlled release of pro-osteogenic bioactive molecules, as well as improved the hydrogel matrix properties, which suggests that these soft nanoparticles can play an important role in bioengineering bioactive nanocomposites for bone tissue engineering in the foreseeable future.

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Section: Resultssupporting

confidence: 82%

Gelatin Methacryloyl (GelMA) Nanocomposite Hydrogels Embedding Bioactive Naringin Liposomes

et al. 2020

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“…Second, our study revealed that NG showed better osteogenesis than CGRP did in some of the groups. The reason might be that NG has anti‐inflammatory properties that can promote the osteoblast process (Shao et al, 2019; Yin et al, 2015). According to these results, 3D bioprinting scaffolds can be fabricated to elucidate the functions of promoting soft and hard tissue regeneration.…”

Section: Discussionmentioning

confidence: 99%

Drug‐loading three‐dimensional scaffolds based on hydroxyapatite‐sodium alginate for bone regeneration

Liang

Huang

et al. 2020

J Biomedical Materials Res

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Bone tissue engineering is a promising approach for tackling clinical challenges. Osteoprogenitor cells, osteogenic factors, and osteoinductive/osteoconductive scaffolds are employed in bone tissue engineering. However, scaffold materials remain limited due to their source, low biocompatibility, and so on. In this study, a composite hydrogel scaffold composed of hydroxyapatite (HA) and sodium alginate (SA) was manufactured using three-dimensional printing. Naringin (NG) and calcitonin-generelated peptide (CGRP) were used as osteogenic factors in the fabrication of drugloaded scaffolds. Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold. Thus, the HA/SA scaffold could be a biocompatible material with potential applications in bone regeneration. Meanwhile, NG and CGRP doping could result in better and more positive proliferation and differentiation.

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“… 54 The morphologies of the MSCs/ECs in PG/PGM were observed using a scanning electron microscope (Nova Nano 450, FEI, USA) as described in our previous study. 55 ECs were fixed in 4% paraformaldehyde (Biosharp, China) and stained with a mouse anti-CD31 monoclonal antibody (Abcam, UK), rhodamine-phalloidin (Cytoskeleton, USA), and DAPI (Vector, USA).…”

Section: Methodsmentioning

confidence: 99%

A hierarchical vascularized engineered bone inspired by intramembranous ossification for mandibular regeneration

Wang

et al. 2022

Int J Oral Sci

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Mandibular defects caused by injuries, tumors, and infections are common and can severely affect mandibular function and the patient’s appearance. However, mandible reconstruction with a mandibular bionic structure remains challenging. Inspired by the process of intramembranous ossification in mandibular development, a hierarchical vascularized engineered bone consisting of angiogenesis and osteogenesis modules has been produced. Moreover, the hierarchical vascular network and bone structure generated by these hierarchical vascularized engineered bone modules match the particular anatomical structure of the mandible. The ultra-tough polyion complex has been used as the basic scaffold for hierarchical vascularized engineered bone for ensuring better reconstruction of mandible function. According to the results of in vivo experiments, the bone regenerated using hierarchical vascularized engineered bone is similar to the natural mandibular bone in terms of morphology and genomics. The sonic hedgehog signaling pathway is specifically activated in hierarchical vascularized engineered bone, indicating that the new bone in hierarchical vascularized engineered bone underwent a process of intramembranous ossification identical to that of mandible development. Thus, hierarchical vascularized engineered bone has a high potential for clinical application in mandibular defect reconstruction. Moreover, the concept based on developmental processes and bionic structures provides an effective strategy for tissue regeneration.

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Controlled Release of Naringin in GelMA-Incorporated Rutile Nanorod Films to Regulate Osteogenic Differentiation of Mesenchymal Stem Cells

Cited by 26 publications

References 32 publications

Gelatin Methacryloyl (GelMA) Nanocomposite Hydrogels Embedding Bioactive Naringin Liposomes

Gelatin Methacryloyl (GelMA) Nanocomposite Hydrogels Embedding Bioactive Naringin Liposomes

Drug‐loading three‐dimensional scaffolds based on hydroxyapatite‐sodium alginate for bone regeneration

A hierarchical vascularized engineered bone inspired by intramembranous ossification for mandibular regeneration

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