Controlling Mesopore Size and Processability of Transparent Enzyme-Loaded Silica Films for Biosensing Applications

Pérez-Anguiano, Oswaldo; Pugin, Raphaël; Hofmann, Heinrich; Scolan, Emmanuel

doi:10.1021/am508630c

Cited by 13 publications

(9 citation statements)

References 80 publications

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“…We fabricated meso‐ and multiscale porous silica films from an aqueous, polymer‐stabilized suspension of silica nanopowders consisting of aggregated primary silica nanoparticles of 5–30 nm in diameter, forming aggregates of 200–300 nm size. These primary aggregates provide a template‐free way to assemble an interconnected silica matrix covering the upper end of the mesoporous size range; it is therefore not necessary to use dedicated mesopore templates such as block copolymer micelles . Second, to create the larger macropore population, we used sacrificial polymer particles to template well‐defined, discrete macropores with high structural integrity.…”

Section: Resultsmentioning

confidence: 99%

“…Enzyme‐based biosensing is an area of great importance in areas as varied as point‐of‐care diagnostics, clinical monitoring, bioprocess technology, winemaking, or food processing . Multiscale porosity has become a fundamental feature for materials used as enzyme supports in biosensing applications.…”

Section: Introductionmentioning

confidence: 99%

“…Our research has focused on the development of robust methodologies for the fabrication of optically transparent multiscale porous silica films, targeting the immobilization of enzymes for biosensing applications. We have previously reported on the synthesis of large mesoporous and transparent silica films synthesized by a bottom‐up approach combining sol–gel chemistry and block‐copolymer self‐assembly . The unique and robust method for the fabrication of these films yielded responsive surfaces with tunable and homogeneously distributed pores from 20 to 100 nm.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transparent and Robust Silica Coatings with Dual Range Porosity for Enzyme‐Based Optical Biosensing

Pérez-Anguiano

Pugin

Scolan

et al. 2017

Adv Funct Materials

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Hierarchically porous transparent silica coatings combine large specific surface area with enhanced pore accessibility for optical biosensing. This paper describes a versatile approach to fabricate optically transparent silica coatings with multiscale porosity. Thin films (around 1 μm in thickness) of an aqueous suspension of primary silica aggregates form a mesoporous, interconnected matrix, and sacrificial polymer particles template well‐defined, discrete macropores with high structural integrity. The total surface area achieved is around 200 m2 g−1 with mesopore sizes of 20–40 nm and macropores of 250 nm, with a total porosity of 84%. The macro/meso dual range of porosity allows enhanced biocatalyst loadings of l‐lactate dehydrogenase for detection of lactate. The functionalized films showed a linear response within the range of interest of 1–20 × 10−3m of lactate. These biosensing coatings therefore strongly enhance sensitivity, speed and reliability of optically based lactate detection as compared to classical thin films with monomodal mesopore structure. Particle‐based simulations and experiments reveal that both the location and connectivity of the macropores control the biosensing performance. The coatings and procedure presented here are versatile, scalable, inexpensive, and are therefore compatible with a wide range of deposition techniques suitable for industrial and health care applications.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transparent and Robust Silica Coatings with Dual Range Porosity for Enzyme‐Based Optical Biosensing

Pérez-Anguiano

Pugin

Scolan

et al. 2017

Adv Funct Materials

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“…Scolan and co‐workers proposed a strategy to reproducibly synthesize transparent porous silica thin films with homogeneously distributed porosity and submicrometer thickness. [ 127 ] Amphiphilic block copolymer polystyrene‐poly‐2‐vinylpyridine (PS‐P2VP) was applied as SDA in the synthesis of mesoporous silica films through EISA. By tuning the alcohol‐based solvent and the block copolymer weight ratio, they obtained a series of thin films with large mesopores up to small macropores (20‐100 nm), which are suitable for enzyme immobilization.…”

Section: Emerging Bioapplications Of Porous Silica‐based Materialsmentioning

confidence: 99%

Sol–Gel‐Based Advanced Porous Silica Materials for Biomedical Applications

Lei

Guo

Noureddine

et al. 2020

Adv Funct Materials

168

109

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Porous silica-based materials have burgeoning applications ranging from fillers and additives, to adsorbents, catalysts, and recently therapeutic agents and vaccines in nanomedicine. The preponderance of these materials is made by sol-gel processing wherein soluble silica precursors are reacted to form amorphous networks composed of siloxane bonds. The facile sol-gel approach allows for an unlimited variety of binary tertiary and more complex chemical compositions including organic ligands and networks resulting in so-called organic-inorganic hybrid materials. Here, a brief review of the recent progress in sol-gel-derived silica materials prepared as particles, thin films, biosilica/silica bioreplicas (of molecules, cells and organisms), and their related preparation, properties, and bioapplications is provided. First, it highlights the recent achievements of mesoporous silica nanoparticles in biomedical applications, including therapeutic agent delivery, multimodal imaging and theranostics, and bone tissue engineering and repair. Second, the research in evaporation-induced self-assembly (EISA)-based mesostructured silica thin films and cell-directed EISA in bio/nano interfaces for various bioapplications, such as bioactive coatings, biosensing, and living cell immobilization, has been reviewed. Third, the pioneering work in biomimetic silicification/ immobilization of biomolecules and bio-organisms and silica bioreplication of complex bio-organisms is summarized. Finally, it is concluded with personal perspectives on the directions of future work on this field. In 1846, Ebelmen synthesized tetraethylorthosilicate (TEOS) from SiCl 4 and ethanol and exposed it to water forming a transparent glassy material upon gelation and drying (now recognized to be a microporous xerogel). In 1864, the term "sol-gel" was first proposed by Graham during his work on silica sols. [2] In 1912-1915, Patrick developed an economically viable and rapid sol-gel process to mass-produce silica gel from sodium silicate (Na 2 SiO 3). And in 1931, Kistler reported the first synthesis of a highly porous silica (SiO 2) form, termed as "aerogel," which was a porous ultralight material derived from hydrolytic polycondensation of silicic acid (Si(OH) 4) to form a gel followed by supercritical drying to avoid drying shrinkage. [3] All of these efforts can be regarded as the beginning of modern sol-gel based silica materials, which are now ripening for wide range of applications, including the two pillars of the chemistry practice (synthesis and analysis), protective coatings, adsorption, chromatography, separation, biotechnology, energy conservation, cultural heritage restoration, and environmental remediation as well as many other fields of contemporary technology. [4] Based on the breakthroughs in synthesis, the recent two decades have witnessed an exponential increase in research of sol-gel-based silica materials for biomedical applications. Silica is inherently compatible with biological systems and was accepted as "Generally Recognized As Saf...

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“…Organic and inorganic groups, antimicrobial compounds [ 69 ], small biomolecules [ 70 , 71 ], or ions can be attached to mesoporous thin films, in order to change the material properties ( Figure 1 ).…”

Section: Functionalization Protocolsmentioning

confidence: 99%

Functionalized Mesoporous Thin Films for Biotechnology

2021

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Mesoporous materials bear great potential for biotechnological applications due to their biocompatibility and versatility. Their high surface area and pore interconnection allow the immobilization of molecules and their subsequent controlled delivery. Modifications of the mesoporous material with the addition of different chemical species, make them particularly suitable for the production of bioactive coatings. Functionalized thin films of mesoporous silica and titania can be used as scaffolds with properties as diverse as promotion of cell growth, inhibition of biofilms formation, or development of sensors based on immobilized enzymes. The possibility to pattern them increase their appeal as they can be incorporated into devices and can be tailored both with respect to architecture and functionalization. In fact, selective surface manipulation is the ground for the fabrication of advanced micro devices that combine standard micro/nanofluids with functional materials. In this review, we will present the advantages of the functionalization of silica and titania mesoporous materials deposited in thin film. Different functional groups used to modify their properties will be summarized, as well as functionalization methods and some examples of applications of modified materials, thus giving an overview of the essential role of functionalization to improve the performance of such innovative materials.

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Controlling Mesopore Size and Processability of Transparent Enzyme-Loaded Silica Films for Biosensing Applications

Cited by 13 publications

References 80 publications

Transparent and Robust Silica Coatings with Dual Range Porosity for Enzyme‐Based Optical Biosensing

Transparent and Robust Silica Coatings with Dual Range Porosity for Enzyme‐Based Optical Biosensing

Sol–Gel‐Based Advanced Porous Silica Materials for Biomedical Applications

Functionalized Mesoporous Thin Films for Biotechnology

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