2013
DOI: 10.1371/journal.pone.0057011
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Convenient Detection of the Citrus Greening (Huanglongbing) Bacterium ‘Candidatus Liberibacter asiaticus’ by Direct PCR from the Midrib Extract

Abstract: A phloem-limited bacterium, ‘Candidatus Liberibacter asiaticus’ (Las) is a major pathogen of citrus greening (huanglongbing), one of the most destructive citrus diseases worldwide. The rapid identification and culling of infected trees and budwoods in quarantine are the most important control measures. DNA amplification including conventional polymerase chain reaction (PCR) has commonly been used for rapid detection and identification. However, long and laborious procedures for DNA extraction have greatly redu… Show more

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Cited by 38 publications
(23 citation statements)
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“…Initially, we quantified the absolute bacterial load in different parts of the leaf including the midrib, leaf blade, petiole and stem based on quantitative detection of CD16-00155. In agreement with other reports (Tatineni et al, 2008;Fujikawa et al, 2013), 'Ca. L. asiaticus' was most and consistently abundant in the midrib (data not shown).…”
Section: Asiaticus'-infected Leaves In Response To Glucosesupporting
confidence: 94%
“…Initially, we quantified the absolute bacterial load in different parts of the leaf including the midrib, leaf blade, petiole and stem based on quantitative detection of CD16-00155. In agreement with other reports (Tatineni et al, 2008;Fujikawa et al, 2013), 'Ca. L. asiaticus' was most and consistently abundant in the midrib (data not shown).…”
Section: Asiaticus'-infected Leaves In Response To Glucosesupporting
confidence: 94%
“…The qPCR results were further confirmed by cPCR using OI1/O12c primers (Jagoueix et al, 1996) and Las606/LSS primers (Fujikawa and Iwanami, 2012;Fujikawa et al, 2013) that amplify 16S ribosomal DNA (rDNA) sequences of Ca. L. asiaticus.…”
Section: Dna Isolationmentioning
confidence: 80%
“…The PCR method has been used to identify CVPD infection in mandarin (Citrus reticulata) fruit based on physical and biochemical characteristic (Shokrollah et al, 2011). The PCR method has been claimed to be a rapid detection and identification of CVPD infection in citrus (Fujikawa et al, 2013;Kawabe et al, 2006). The Random Amplified Polymorphic DNA (RAPD) method has also been used to identify polymorphic DNA fragments of an infected citrus by the bacteria (Hocquellet et al, 1999).…”
Section: Introductionmentioning
confidence: 99%