Conversion efficiency of glucose/xylose mixtures for ethanol production using Saccharomyces cerevisiae ITV01 and Pichia stipitis NRRL Y‐7124

Abstract

BACKGROUND: Efficient conversion of glucose/xylose mixtures from lignocellulose is necessary for commercially viable ethanol production. Oxygen and carbon sources are of paramount importance for ethanol yield. The aim of this work was to evaluate different glucose/xylose mixtures for ethanol production using S. cerevisiae ITV-01 (wild type yeast) and P. stipitis NRRL Y-7124 and the effect of supplying oxygen in separate and co-culture processes.

Cell-recycle batch process of Scheffersomyces stipitis and Saccharomyces cerevisiae co-culture for second generation bioethanol production

“…Moreover, this co-culture exhibited the best ethanol productivity (0.186 g l -1 h -1 ) and ethanol yield (0.336 g g -1 ). These results are partially comparable with those achieved by Gutiérrez-Rivera et al (2011). These authors obtained about 30 g ethanol l -1 with a yield of 0.306 g ethanol g -1 in shaker flasks trials using a syrup containing 75 g glucose l -1 and 30 g xylose l -1 and a co-culture of Sacc.…”

Production and purification of xylitol by Scheffersomyces amazonenses via sugarcane hemicellulosic hydrolysate

“…(a)), while arabinose (data not shown) was not assimilated by the yeast. The assimilation of arabinose by other species of genus Scheffersomyces is not very common, and similar behavior was also observed in S. stipitis , which is commonly used for producing bioethanol from hemicellulosic hydrolysates …”

“…The assimilation of arabinose by other species of genus Scheffersomyces is not very common, 34,35 and similar behavior was also observed in S. stipitis, which is commonly used for producing bioethanol from hemicellulosic hydrolysates. [36][37][38][39] Consumption of 96% of the acetic acid was also observed at 96 h (see Fig. 3(a)).…”

Ethanol from Biomass Hydrolysates by Efficient Fermentation of Glucose and Xylose – A Review

“…Gutiérrez‐Rivera et al. showed that the oxygen present in the glucose/xylose mixture promotes complete sugar consumption by P. stipitis resulting in improved ethanol production. However, in co‐cultures with S. cerevisiae under aerobic conditions, a 20.4 % xylose residue was caused by oxygen limitation and ethanol inhibition by P. stipitis.…”

“…The co‐culture process of P. stipitis mutants with native strains of S. cerevisiae using glucose and xylose for bioethanol production yields 0.28 to 0.40 g EtOH g −1 compared to growth in glucose alone (0.38 to 0.45 g EtOH g −1 ) . In co‐consumption, S. cerevisiae and P. stipitis showed a five‐fold increase in the volumetric ethanol productivity compared to the respective mono‐cultures . In a co‐culture of S. cerevisiae and P. stipitis , it was difficult to control q O2 at an optimum value for xylose fermentation by P. stipitis because oxygen was consumed primarily by S. cerevisiae .…”