Conversion of chitinous wastes to hydrogen gas by clostridium paraputrificum M-21

Evvyernie, D; Morimoto, Kenji; Karita, Shuichi; Kimura, Takeshi; Sakka, Kazuo; Ohmiya, Kunio

doi:10.1016/s1389-1723(01)80148-1

Cited by 96 publications

(19 citation statements)

References 19 publications

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“…Although C. paraputrificum M‐21 also produced an enormous volume of hydrogen gas during the exponential growth phase [8,9], the yield of hydrogen gas from 1 mol of glucose did not reach 2.4 mol (1.4 mol). When C. paraputrificum M‐21 was cultured with 1% GlcNAc, not only acetic and butyric acids but also lactic acid was produced from GlcNAc (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of a hydrogenase gene inClostridium paraputrificumto enhance hydrogen gas production

Morimoto¹,

Kimura²,

Sakka³

et al. 2005

FEMS Microbiology Letters

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114

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A [Fe]-hydrogenase gene (hydA) was cloned from Clostridium paraputrificum M-21 in Escherichia coli using a conserved DNA sequence of clostridial hydrogenase genes amplified by PCR as the probe. The hydA gene consisted of an open reading frame of 1749 bp encoding 582 amino acids with an estimated molecular mass of 64,560 Da. It was ligated into a shuttle vector, pJIR751, originally constructed for Clostridium perfringens and E. coli, and expressed in C. paraputrificum. Hydrogen gas productivity of the recombinant increased up to 1.7-fold compared with the wild-type. In the recombinant, overexpression of hydA abolished lactic acid production and increased acetic acid production by over-oxidation of NADH, which is required for reduction of pyruvic acid to lactic acid in the wild-type.

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Section: Discussionmentioning

confidence: 99%

Overexpression of a hydrogenase gene inClostridium paraputrificumto enhance hydrogen gas production

Morimoto¹,

Kimura²,

Sakka³

et al. 2005

FEMS Microbiology Letters

Self Cite

114

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“…According to our search, there is only one report on hydrogen production from chitin or chitin-containing materials. Evvyernie et al has reported hydrogen production from a mesophilic bacterium, Clostridium paraputrificum M-21, using N-acetylglucosamine, chitin, and chitincontaining wastes (45,46). From 1 g of raw chitinous wastes, the evolved hydrogen was reported as 5.2 to 7.6 mmol.…”

Section: Discussionmentioning

confidence: 99%

Engineering of the Hyperthermophilic Archaeon Thermococcus kodakarensis for Chitin-Dependent Hydrogen Production

Aslam

Horiuchi

Simons

et al. 2017

Appl Environ Microbiol

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Thermococcus kodakarensis is a hyperthermophilic archaeon that harbors a complete set of genes for chitin degradation to fructose 6-phosphate. However, wild-type T. kodakarensis KOD1 does not display growth on chitin. In this study, we developed a T. kodakarensis strain that can grow on chitin via genetic and adaptive engineering. First, a chitinase overproduction strain (KC01) was constructed by replacing the chitinase gene promoter with a strong promoter from the cell surface glycoprotein gene, resulting in increased degradation of swollen chitin and accumulation of N-,N=-diacetylchitobiose in the medium. To enhance N-,N=-diacetylchitobiose assimilation in KC01, genes encoding diacetylchitobiose deacetylase, exo-␤-D-glucosaminidase, and glucosamine-6-phosphate deaminase were also overexpressed to obtain strain KC04. To strengthen the glycolytic flux of KC04, the gene encoding Tgr (transcriptional repressor of glycolytic genes) was disrupted to obtain strain KC04Δt. In both KC04 and KC04Δt strains, degradation of swollen chitin was further enhanced. In the culture broth of these strains, the accumulation of glucosamine was observed. KC04Δt was repeatedly inoculated in a swollen-chitin-containing medium for 13 cultures. This adaptive engineering strategy resulted in the isolation of a strain (KC04ΔtM1) that showed almost complete degradation of 0.4% (wt/vol) swollen chitin after 90 h. The strain produced high levels of acetate and ammonium in the culture medium, and, moreover, molecular hydrogen was generated. This strongly suggests that strain KC04ΔtM1 has acquired the ability to convert chitin to fructose 6-phosphate via deacetylation and deamination and further convert fructose 6-phosphate to acetate via glycolysis coupled to hydrogen generation. IMPORTANCE Chitin is a linear homopolymer of ␤-1,4-linked N-acetylglucosamine and is the second most abundant biomass next to cellulose. Compared to the wealth of research focused on the microbial degradation and conversion of cellulose, studies addressing microbial chitin utilization are still limited. In this study, using the hyperthermophilic archaeon Thermococcus kodakarensis as a host, we have constructed a strain that displays chitin-dependent hydrogen generation. The apparent hydrogen yield per unit of sugar consumed was slightly higher with swollen chitin than with starch. As gene manipulation in T. kodakarensis is relatively simple, the strain constructed in this study can also be used as a parent strain for the development and expansion of chitin-dependent biorefinery, in addition to its capacity to produce hydrogen.

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“…Rohit and Evvyernie used activated sludge as the source of the strain and studied the hydrogen production characteristics of the straw by acidification and high‐temperature pretreatment, respectively . Later, many scholars studied the combined fermentation process of straw and cow‐dung‐mixed matrix . In order to investigate the characteristics of the production of biogenic gas from lignite with acid‐alkali pretreatment, Xia et al carried out the fermentation experimental of hydrogen and methane production by using the cultured exogenous bacteria…”

Section: Introductionmentioning

confidence: 99%

“…7,8 Later, many scholars studied the combined fermentation process of straw and cow-dung-mixed matrix. 9,10 In order to investigate the characteristics of the production of biogenic gas from lignite with acid-alkali pretreatment, Xia et al carried out the fermentation experimental of hydrogen and methane production by using the cultured exogenous bacteria. 11 Anaerobic fermentation conditions have an important impact on the growth and metabolism of microorganisms, substrate utilization, and hydrogen production capacity.…”

mentioning

confidence: 99%

The variety and transition of key intermediate liquid products during the process of coal‐to‐biohydrogen fermentation

Jiangtao

Xia

et al. 2018

Int J Energy Res

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Summary Organic liquid intermediates were generated during the coal‐to‐biohydrogen fermentation process. The study on the generation of these organic liquid intermediates can provide information on the biogenic gas formation mechanism. In this paper, high volatile bituminous coal from Yima colliery was collected as an effective carbon source. The indigenous microflora extracted from a coal mine water was used as the bacterial source to set up simulation experiment to get biogenic gas. Then, gas chromatography and ultraviolet spectrophotometry, combined with liquid‐liquid extraction gas chromatography–mass spectrometry, were used to analyze the gas composition, as well as the production of saccharide, pyruvic acid, and volatile intermediates in the bioreactor fluids. The results show that the gases generated from the fermentation experiment contained hydrogen, carbon dioxide, and nitrogen. Twelve volatile component types were detected in the bioreactor fluids: straight alkanes, branched alkanes, alkenes, alcohols, aldehydes, ketones, acids, lipids, amines, quinolones, phenols, and epoxy alkanes. The key components of organic intermediates produced were saccharides, pyruvic acid, organic acids and alcohol, straight alkanes, and amide. Saccharides, pyruvic acid, alkanes, and organic acids were closely related to the generation of hydrogen and carbon dioxide, whereas amide stimulated nitrogen generation. Three stages are divided in the fermentation process. The first stage is hydrolysis, where the total, reducing, and polysaccharides production reached a maximum, with generation of long‐chain fatty acids as well as other liquid organics. The second stage is hydrogen production stage, which includes peak hydrogen production and hydrogen production stabilization stages. In the peak hydrogen production stage, saccharide production rapidly decreases, with an initial increase and subsequent decrease in pyruvic acid, straight alkanes, and branched alkanes. With the production of acetic acid and hydrogen, carbon dioxide production was initially slow but then rapidly increases. In stabilization of hydrogen generation stage, the saccharides were mostly consumed, pyruvic acid and branched alkanes' production increased slowly, but straight alkanes' production continued to decrease. In this stage, the production of acetic acid, hydrogen, and carbon dioxide was relatively stable, and the production of amide and nitrogen increased slowly. The third stage is the homoacetogenic stage, where pyruvic acid and branched alkane production continued to increase and straight alkanes' production continued to decrease. The pyruvic acid production increased abnormally, while amide and nitrogen production began to decrease and gas production nearly terminates. The liquid phase intermediate plays an important role in the coal‐fermenting hydrogen generation process and in revealing the hydrogen generation mechanism.

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Conversion of chitinous wastes to hydrogen gas by clostridium paraputrificum M-21

Cited by 96 publications

References 19 publications

Overexpression of a hydrogenase gene inClostridium paraputrificumto enhance hydrogen gas production

Overexpression of a hydrogenase gene inClostridium paraputrificumto enhance hydrogen gas production

Engineering of the Hyperthermophilic Archaeon Thermococcus kodakarensis for Chitin-Dependent Hydrogen Production

The variety and transition of key intermediate liquid products during the process of coal‐to‐biohydrogen fermentation

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