Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered Bacillus subtilis

Kalantari, Aida; Chen, Tao; Ji, Boyang; Stančik, Ivan Andreas; Ravikumar, Vaishnavi; Franjević, Damjan; Saulou‐Bérion, Claire; Goelzer, Anne; Mijaković, Ivan

doi:10.3389/fmicb.2017.00638

Cited by 24 publications

(27 citation statements)

References 53 publications

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“…Another host strain that has been used is Bacillus subtilis, which shows high growth rates, and is a non-pathogenic microorganism classified as GRAS (generally recognized as safe) [42]. The first attempt to use this host microorganism for 3HP was described by Kalantari et al [42].…”

Section: Other Microorganismsmentioning

confidence: 99%

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Biological Production of 3-Hydroxypropionic Acid: An Update on the Current Status

et al. 2018

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Abstract:The production of high added-value chemicals from renewable resources is a necessity in our attempts to switch to a more sustainable society. 3-Hydroxypropionic acid (3HP) is a promising molecule that can be used for the production of an important array of high added-value chemicals, such as 1,3-propanediol, acrylic acid, acrylamide, and bioplastics. Biological production of 3HP has been studied extensively, mainly from glycerol and glucose, which are both renewable resources. To enable conversion of these carbon sources to 3HP, extensive work has been performed to identify appropriate biochemical pathways and the enzymes that are involved in them. Novel enzymes have also been identified and expressed in host microorganisms to improve the production yields of 3HP. Various process configurations have also been proposed, resulting in improved conversion yields. The intense research efforts have resulted in the production of as much as 83.8 g/L 3HP from renewable carbon resources, and a system whereby 3-hydroxypropionitrile was converted to 3HP through whole-cell catalysis which resulted in 184.7 g/L 3HP. Although there are still challenges and difficulties that need to be addressed, the research results from the past four years have been an important step towards biological production of 3HP at the industrial level.

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Section: Other Microorganismsmentioning

confidence: 99%

“…The first attempt to use this host microorganism for 3HP was described by Kalantari et al [42]. The constructed strain performed well in shaker flasks, producing up to 10 g/L with average yield 0.79 g/g, and showing good tolerance towards the 3HP produced.…”

Section: Other Microorganismsmentioning

confidence: 99%

Biological Production of 3-Hydroxypropionic Acid: An Update on the Current Status

et al. 2018

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“…This CoA-independent pathway was also recently implemented in the industrial chassis Bacillus subtilis (Kalantari et al, 2017). B. subtilis was transformed with the two-step pathway from K. pneumoniae.…”

Section: Glycerol Oxidation Through a Coenzyme A-independent Pathwaymentioning

confidence: 99%

Process engineering for microbial production of 3-hydroxypropionic acid

Fouchécour

Sánchez-Castañeda

Saulou‐Bérion

et al. 2018

Biotechnology Advances

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Due to concerns about the unsustainability and predictable shortage of fossil feedstocks, research efforts are currently being made to develop new processes for production of commodities using alternative feedstocks. 3-Hydroxypropionic acid (CAS 503-66-2) was recognised by the US Department of Energy as one of the most promising value-added chemicals that can be obtained from biomass. This article aims at reviewing the various strategies implemented thus far for 3-hydroxypropionic acid bioproduction. Special attention is given here to process engineering issues. The variety of possible metabolic pathways is also described in order to highlight how process design can be guided by their understanding. The most recent advances are described here in order to draw up a panorama of microbial 3-hydroxypropionic acid production: best performances to date, remaining hurdles and foreseeable developments. Important milestones have been achieved, and process metrics are getting closer to commercial relevance. New strategies are continuously being developed that involve new microbial strains, new technologies, or new carbon sources in order to overcome the various hurdles inherent to the different microbial routes.

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“…This pathway allows higher theoretical maximum yield of 0.97 g/g glycerol compared to other 3‐HP biosynthetic pathways (Jung, Kang, Chu, Choi, & Cho, 2014). Thus, this biosynthetic pathway is widely used for 3‐HP production using various microorganisms such as Escherichia coli (Chu et al, 2015; Jung et al, 2014), Klebsiella pneumoniae (Li, Wang, Ge, & Tian, 2016; Zhao, Ma, Xu, & Tian, 2019), Pseudomonas denitrificans (Zhou, Ashok, Ko, Kim, & Park, 2014), Lactobacillus reutri (Zaushitsyna, Dishisha, Hatti‐Kaul, & Mattiasson, 2017), Bacillus subtilis (Kalantari et al, 2017), Corynebacterium glutamicum (Chen et al, 2017), and Saccharomyces cerevisiae (Borodina et al, 2015; Kildegaard, Wang, Chen, Nielsen, & Borodina, 2015).…”

Section: Introductionmentioning

confidence: 99%

High‐level production of 3‐hydroxypropionic acid from glycerol as a sole carbon source using metabolically engineered Escherichia coli

Kim

Chae

et al. 2020

Biotech & Bioengineering

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As climate change is an important environmental issue, the conventional petrochemical‐based processes to produce valuable chemicals are being shifted toward eco‐friendly biological‐based processes. In this study, 3‐hydroxypropionic acid (3‐HP), an industrially important three carbon (C3) chemical, was overproduced by metabolically engineered Escherichia coli using glycerol as a sole carbon source. As the first step to construct a glycerol‐dependent 3‐HP biosynthetic pathway, the dhaB1234 and gdrAB genes from Klebsiella pneumoniae encoding glycerol dehydratase and glycerol reactivase, respectively, were introduced into E. coli to convert glycerol into 3‐hydroxypropionaldehyde (3‐HPA). In addition, the ydcW gene from K. pneumoniae encoding γ‐aminobutyraldehyde dehydrogenase, among five aldehyde dehydrogenases examined, was selected to further convert 3‐HPA to 3‐HP. Increasing the expression level of the ydcW gene enhanced 3‐HP production titer and reduced 1,3‐propanediol production. To enhance 3‐HP production, fed‐batch fermentation conditions were optimized by controlling dissolved oxygen (DO) level and employing different feeding strategies including intermittent feeding, pH‐stat feeding, and continuous feeding strategies. Fed‐batch culture of the final engineered E. coli strain with DO control and continuous feeding strategy produced 76.2 g/L of 3‐HP with the yield and productivity of 0.457 g/g glycerol and 1.89 g·L−1·h−1, respectively. To the best of our knowledge, this is the highest 3‐HP productivity achieved by any microorganism reported to date.

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Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered Bacillus subtilis

Cited by 24 publications

References 53 publications

Biological Production of 3-Hydroxypropionic Acid: An Update on the Current Status

Biological Production of 3-Hydroxypropionic Acid: An Update on the Current Status

Process engineering for microbial production of 3-hydroxypropionic acid

High‐level production of 3‐hydroxypropionic acid from glycerol as a sole carbon source using metabolically engineered Escherichia coli

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