Conversion of waste cooking oil into medium chain polyhydroxyalkanoates in a high cell density fermentation

Ruiz, Carolina; Kenny, Shane T.; Narancic, Tanja; Babu, Ramesh; Connor, Kevin O’

doi:10.1016/j.jbiotec.2019.08.020

Cited by 72 publications

(39 citation statements)

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“…Therefore, this work demonstrated a novel method of simultaneously producing biofuels alongside PHAs using renewable and readily available feedstocks such as food waste. Other authors reported the production of PHAs using VFAs that are derived from lipid-rich wastes such as waste cooking oils [49], palm oils [50], waste animal fats [51], and olive mill effluents [52] are also expected to play a pivotal role in accelerating the production of PHAs from waste materials. In summary, the production of PHAs using VFAs derived from waste feedstocks may be both beneficial and economical because this technology generates industrially-relevant biocompounds that have diverse applications while alleviating environmental problems (Table 2).…”

Section: Polyhydroxyalkanoatesmentioning

confidence: 99%

Valorization of volatile fatty acids from the dark fermentation waste Streams-A promising pathway for a biorefinery concept

Sekoai

Ghimire

Ezeokoli

et al. 2021

Renewable and Sustainable Energy Reviews

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In recent years, much attention has been directed towards the integration of dark fermentation process into a biorefinery concept to enhance the energetic gains, thereby improving the competitiveness of this process. The volatile fatty acids (VFAs) from dark fermentative H 2 -producing processes serve as precursors for the microbial synthesis of a broad spectrum of biotechnologically-important products such as biofuels and biocommodities. These products are desirable substrates for secondary bioprocesses due to their biodegradable nature and affordability. This short review discusses the use of acidogenic-derived VFAs in the production of value-added compounds such as polyhydroxyalkanoates (PHAs) alongside the microbial-based fuels (hydrogen, biogas, and electricity), and other valuable compounds (succinic acid, citric acid, and butanol). The review also highlights the strategies that have been used to enhance the extraction of VFAs from acidogenic effluents and other related waste streams. The application of novel enhancement techniques such as nanoparticles during VFAs recovery is also discussed in this work. Furthermore, the work highlights some of the recent advances in dark fermentationbased biorefinery, particularly the development of pilot-scale processes. Finally, the review provides some suggestions on the advancement of dark fermentation-based biorefineries using VFAs that are derived from acidogenic processes.

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Section: Polyhydroxyalkanoatesmentioning

confidence: 99%

Valorization of volatile fatty acids from the dark fermentation waste Streams-A promising pathway for a biorefinery concept

Sekoai

Ghimire

Ezeokoli

et al. 2021

Renewable and Sustainable Energy Reviews

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“…Ruiz et al, using P. chlororaphis 555 as the production strain, developed a HCDC bioreactor-based process to convert WCOs from restaurants to mcl-PHA. The composition of the WCO is palmitic acid (7.9%), stearic acid (42.3%), oleic acid (42.3%), linoleic acid (32.2%), and others (0.7%) [ 251 ]. In batch bioreactor experiments, when 60 g/L of WCO was supplied during a 30 h fermentation, the total biomass, %-mcl-PHA content in the CDW and overall productivity reached 45.5 g/L, 19.8%, and 0.30 g/L/h [ 251 ].…”

Section: Industrial/agro-industrial Waste For Production Of Scl-and Mcl-phamentioning

confidence: 99%

“…The composition of the WCO is palmitic acid (7.9%), stearic acid (42.3%), oleic acid (42.3%), linoleic acid (32.2%), and others (0.7%) [ 251 ]. In batch bioreactor experiments, when 60 g/L of WCO was supplied during a 30 h fermentation, the total biomass, %-mcl-PHA content in the CDW and overall productivity reached 45.5 g/L, 19.8%, and 0.30 g/L/h [ 251 ]. The authors then implemented a pulse feeding strategy controlled by the DO-stat.…”

Section: Industrial/agro-industrial Waste For Production Of Scl-and Mcl-phamentioning

confidence: 99%

“…They also implemented nutrient limitation (phosphorous) during PHA formation. The resulting cultivation in which 140 g WCO/L was supplied over 48 h resulted in a total biomass, %-PHA content, and overall productivity of 73 g/L, 19%-P(20 mol%3HDD-37 mol%3HD-36 mol%3HO-7 mol%3HHx) and 0.29 g/L/h, respectively [ 251 ].…”

Section: Industrial/agro-industrial Waste For Production Of Scl-and Mcl-phamentioning

confidence: 99%

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Emergent Approaches to Efficient and Sustainable Polyhydroxyalkanoate Production

2021

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Petroleum-derived plastics dominate currently used plastic materials. These plastics are derived from finite fossil carbon sources and were not designed for recycling or biodegradation. With the ever-increasing quantities of plastic wastes entering landfills and polluting our environment, there is an urgent need for fundamental change. One component to that change is developing cost-effective plastics derived from readily renewable resources that offer chemical or biological recycling and can be designed to have properties that not only allow the replacement of current plastics but also offer new application opportunities. Polyhydroxyalkanoates (PHAs) remain a promising candidate for commodity bioplastic production, despite the many decades of efforts by academicians and industrial scientists that have not yet achieved that goal. This article focuses on defining obstacles and solutions to overcome cost-performance metrics that are not sufficiently competitive with current commodity thermoplastics. To that end, this review describes various process innovations that build on fed-batch and semi-continuous modes of operation as well as methods that lead to high cell density cultivations. Also, we discuss work to move from costly to lower cost substrates such as lignocellulose-derived hydrolysates, metabolic engineering of organisms that provide higher substrate conversion rates, the potential of halophiles to provide low-cost platforms in non-sterile environments for PHA formation, and work that uses mixed culture strategies to overcome obstacles of using waste substrates. We also describe historical problems and potential solutions to downstream processing for PHA isolation that, along with feedstock costs, have been an Achilles heel towards the realization of cost-efficient processes. Finally, future directions for efficient PHA production and relevant structural variations are discussed.

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“…were reported to be capable of utilizing inexpensive oils into PHA MCL . Ruiz et al [ 100 ] conducted pulse fed-batch fermentation using Pseudomonas chlororaphis 555 grown on waste cooking oil. The authors achieved the highest reported biomass (73 g L −1 ) and PHA volumetric productivity (0.29 g mcl-PHA −1 L −1 h −1 ).…”

Section: Spent Oils As Substrate For Pha Biosynthesismentioning

confidence: 99%

What Is New in the Field of Industrial Wastes Conversion into Polyhydroxyalkanoates by Bacteria?

Marciniak

Możejko-Ciesielska

2021

Polymers

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The rising global consumption and industrialization has resulted in increased food processing demand. Food industry generates a tremendous amount of waste which causes serious environmental issues. These problems have forced us to create strategies that will help to reduce the volume of waste and the contamination to the environment. Waste from food industries has great potential as substrates for value-added bioproducts. Among them, polyhydroxyalkanaotes (PHAs) have received considerable attention in recent years due to their comparable characteristics to common plastics. These biodegradable polyesters are produced by microorganisms during fermentation processes utilizing various carbon sources. Scale-up of PHA production is limited due to the cost of the carbon source metabolized by the microorganisms. Therefore, there is a growing need for the development of novel microbial processes using inexpensive carbon sources. Such substrates could be waste generated by the food industry and food service. The use of industrial waste streams for PHAs biosynthesis could transform PHA production into cheaper and more environmentally friendly bioprocess. This review collates in detail recent developments in the biosynthesis of various types of PHAs produced using waste derived from agrofood industries. Challenges associated with this production bioprocess were described, and new ways to overcome them were proposed.

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Conversion of waste cooking oil into medium chain polyhydroxyalkanoates in a high cell density fermentation

Cited by 72 publications

References 50 publications

Valorization of volatile fatty acids from the dark fermentation waste Streams-A promising pathway for a biorefinery concept

Valorization of volatile fatty acids from the dark fermentation waste Streams-A promising pathway for a biorefinery concept

Emergent Approaches to Efficient and Sustainable Polyhydroxyalkanoate Production

What Is New in the Field of Industrial Wastes Conversion into Polyhydroxyalkanoates by Bacteria?

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