Coordination of the Initiation of Recombination and the Reductional Division in Meiosis in Saccharomyces cerevisiae

Jiao, Kai; Bullard, Steven A.; Salem, Laura; Malone, Robert E.

doi:10.1093/genetics/152.1.117

Cited by 23 publications

(2 citation statements)

References 48 publications

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“…Another difference between yeast and Arabidopsis concerns spo11 mutants, which progress faster through prophase I in the yeast mutant than its WT strain, whereas, as shown in this study, Arabidopsis spo11 mutants are delayed in early prophase in meiosis ( Klapholz et al, 1985 ; Jiao et al, 1999 ; Cha et al, 2000 ). The different behavior of these mutants cannot currently be resolved, but possibly hints at different mechanisms of homolog interaction in yeast versus Arabidopsis.…”

Section: Discussionmentioning

confidence: 52%

Heat stress reveals a specialized variant of the pachytene checkpoint in meiosis of Arabidopsis thaliana

et al. 2021

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Plant growth and fertility strongly depend on environmental conditions such as temperature. Remarkably, temperature also influences meiotic recombination and thus, the current climate change will affect the genetic make-up of plants. To better understand the effects of temperature on meiosis, we followed male meiocytes in Arabidopsis thaliana by live cell imaging under three temperature regimes: at 21°C; at heat shock conditions of 30°C and 34°C; after an acclimatization phase of 1 week at 30°C. This work led to a cytological framework of meiotic progression at elevated temperature. We determined that an increase from 21°C to 30°C speeds up meiosis with specific phases being more amenable to heat than others. An acclimatization phase often moderated this effect. A sudden increase to 34°C promoted a faster progression of early prophase compared to 21°C. However, the phase in which cross-overs mature was prolonged at 34°C. Since mutants involved in the recombination pathway largely did not show the extension of this phase at 34°C, we conclude that the delay is recombination-dependent. Further analysis also revealed the involvement of the ATAXIA TELANGIECTASIA MUTATED kinase in this prolongation, indicating the existence of a pachytene checkpoint in plants, yet in a specialized form.

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Section: Discussionmentioning

confidence: 52%

Heat stress reveals a specialized variant of the pachytene checkpoint in meiosis of Arabidopsis thaliana

et al. 2021

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“…Furthermore, live-cell imaging has also the great potential to contribute to an understanding of species-specific difference in meiosis. For instance, the duration of leptotene and early pachytene was extended in spo11-1 mutants in Arabidopsis while in yeast, the corresponding mutants progress faster through meiosis than the wildtype (Klapholz et al, 1985;Jiao et al, 1999;Cha et al, 2000;De Jaeger-Braet et al, 2021). Thus, the era of live-cell imaging for meiosis has just begun, with many exciting discoveries ahead of us.…”

Section: Meiotic Recombination Observations By Live-cell Imagingmentioning

confidence: 99%

Caught in the Act: Live-Cell Imaging of Plant Meiosis

et al. 2021

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Live-cell imaging is a powerful method to obtain insights into cellular processes, particularly with respect to their dynamics. This is especially true for meiosis, where chromosomes and other cellular components such as the cytoskeleton follow an elaborate choreography over a relatively short period of time. Making these dynamics visible expands understanding of the regulation of meiosis and its underlying molecular forces. However, the analysis of meiosis by live-cell imaging is challenging; specifically in plants, a temporally resolved understanding of chromosome segregation and recombination events is lacking. Recent advances in live-cell imaging now allow the analysis of meiotic events in plants in real time. These new microscopy methods rely on the generation of reporter lines for meiotic regulators and on the establishment of ex vivo culture and imaging conditions, which stabilize the specimen and keep it alive for several hours or even days. In this review, we combine an overview of the technical aspects of live-cell imaging in plants with a summary of outstanding questions that can now be addressed to promote live-cell imaging in Arabidopsis and other plant species and stimulate ideas on the topics that can be addressed in the context of plant meiotic recombination.

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Phylogenetic footprinting reveals multiple regulatory elements involved in control of the meiotic recombination gene, REC102

Jiao

Nau

Cool

et al. 2001

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REC102 is a meiosis-specific early exchange gene absolutely required for meiotic recombination in Saccharomyces cerevisiae. Sequence analysis of REC102 indicates that there are multiple potential regulatory elements in its promoter region, and a possible regulatory element in the coding region. This suggests that the regulation of REC102 may be complex and may include elements not yet reported in other meiotic genes. To identify potential cis-regulatory elements, phylogenetic footprinting analysis was used. REC102 homologues were cloned from other two Saccharomyces spp. and sequence comparison among the three species defined evolutionarily conserved elements. Deletion analysis demonstrated that the early meiotic gene regulatory element URS1 was necessary but not sufficient for proper regulation of REC102. Upstream elements, including the binding sites for Gcr1p, Yap1p, Rap1p and several novel conserved sequences, are also required for the normal regulation of REC102 as well as a Rap1p binding site located in the coding region. The data in this paper support the use of phylogenetic comparisions as a method for determining important sequences in complex promoters.

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Coordination of the Initiation of Recombination and the Reductional Division in Meiosis in Saccharomyces cerevisiae

Cited by 23 publications

References 48 publications

Heat stress reveals a specialized variant of the pachytene checkpoint in meiosis of Arabidopsis thaliana

Heat stress reveals a specialized variant of the pachytene checkpoint in meiosis of Arabidopsis thaliana

Caught in the Act: Live-Cell Imaging of Plant Meiosis

Phylogenetic footprinting reveals multiple regulatory elements involved in control of the meiotic recombination gene, REC102

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