COPI complex isoforms are required for the early acceptance of compatible pollen grains in Arabidopsis thaliana

Gomez, Daniel A Cabada; Chavez, Maria Isabella; Cobos, Alejandra N; Gross, Roni J; Yescas, Julia A; Balogh, Michael; Indriolo, Emily

doi:10.1007/s00497-020-00387-9

Cited by 12 publications

(3 citation statements)

References 50 publications

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“…These groups included glycine-rich proteins, oleosins, expansins, and the cell wall regulators pectinesterases and pectinesterase inhibitors. Also, three subunits of the coatomer complex were up-regulated, epsilon-1 (At1g30630), zeta-2 (At3g09800) and zeta-3 (At4g08520) subunits (Cabada Gomez et al 2020 ). Numerous proteins with a role in pollen germination and pollen tube growth showed enrichment.…”

Section: Resultsmentioning

confidence: 99%

Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis

et al. 2022

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Sexual reproduction in angiosperms requires the production and delivery of two male gametes by a three-celled haploid male gametophyte. This demands synchronized gene expression in a short developmental window to ensure double fertilization and seed set. While transcriptomic changes in developing pollen are known for Arabidopsis, no studies have integrated RNA and proteomic data in this model. Further, the role of alternative splicing has not been fully addressed, yet post-transcriptional and post-translational regulation may have a key role in gene expression dynamics during microgametogenesis. We have refined and substantially updated global transcriptomic and proteomic changes in developing pollen for two Arabidopsis accessions. Despite the superiority of RNA-seq over microarray-based platforms, we demonstrate high reproducibility and comparability. We identify thousands of long non-coding RNAs as potential regulators of pollen development, hundreds of changes in alternative splicing and provide insight into mRNA translation rate and storage in developing pollen. Our analysis delivers an integrated perspective of gene expression dynamics in developing Arabidopsis pollen and a foundation for studying the role of alternative splicing in this model.

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Section: Resultsmentioning

confidence: 99%

Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis

et al. 2022

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“…In plants, COPI is involved in retrograde transport from the Golgi to the ER, Golgi maintenance and cell-plate formation, and its prolonged depletion induces programmed cell death in leaf cells [ 99 ]. A recent study demonstrated that COPI may contribute to the acceptance of compatible pollen grains [ 100 ]. In liverwortare, analysis of a Marchantia polymorpha T-DNA-mutant with LDs with increased circularity confirmed that COPI subunit MpSEC28 is responsible for this phenotype and that normal LD formation requires COPI-mediated secretory activity [ 12 ].…”

Section: Ld Buddingmentioning

confidence: 99%

Dynamic Regulation of Lipid Droplet Biogenesis in Plant Cells and Proteins Involved in the Process

Zhao

Dong

Geng

et al. 2023

IJMS

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Lipid droplets (LDs) are ubiquitous, dynamic organelles found in almost all organisms, including animals, protists, plants and prokaryotes. The cell biology of LDs, especially biogenesis, has attracted increasing attention in recent decades because of their important role in cellular lipid metabolism and other newly identified processes. Emerging evidence suggests that LD biogenesis is a highly coordinated and stepwise process in animals and yeasts, occurring at specific sites of the endoplasmic reticulum (ER) that are defined by both evolutionarily conserved and organism- and cell type-specific LD lipids and proteins. In plants, understanding of the mechanistic details of LD formation is elusive as many questions remain. In some ways LD biogenesis differs between plants and animals. Several homologous proteins involved in the regulation of animal LD formation in plants have been identified. We try to describe how these proteins are synthesized, transported to the ER and specifically targeted to LD, and how these proteins participate in the regulation of LD biogenesis. Here, we review current work on the molecular processes that control LD formation in plant cells and highlight the proteins that govern this process, hoping to provide useful clues for future research.

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“…Double mutant of β 1/2-COP and a single mutant of α 2-COP were dwarfed compared to Col-0 [ 49 , 52 ], and the silencing of β 1/2-COP or α 2-COP or p24 protein altered the structure of the Golgi [ 49 , 52 , 53 ]. The knockout of α 1-COP, γ -COP, and ε -COP resulted in a reduction in seed production due to altered pollen grain adherence and pollen tube germination [ 54 ]. The β ′-, γ -, and δ -COP proteins were shown to interact with each other and were localized in Golgi [ 55 ].…”

Section: Main Components Of the Secretory Pathwaymentioning

confidence: 99%

Critical Determinants in ER-Golgi Trafficking of Enzymes Involved in Glycosylation

Zhang

Zabotina

2022

Plants

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All living cells generate structurally complex and compositionally diverse spectra of glycans and glycoconjugates, critical for organismal evolution, development, functioning, defense, and survival. Glycosyltransferases (GTs) catalyze the glycosylation reaction between activated sugar and acceptor substrate to synthesize a wide variety of glycans. GTs are distributed among more than 130 gene families and are involved in metabolic processes, signal pathways, cell wall polysaccharide biosynthesis, cell development, and growth. Glycosylation mainly takes place in the endoplasmic reticulum (ER) and Golgi, where GTs and glycosidases involved in this process are distributed to different locations of these compartments and sequentially add or cleave various sugars to synthesize the final products of glycosylation. Therefore, delivery of these enzymes to the proper locations, the glycosylation sites, in the cell is essential and involves numerous secretory pathway components. This review presents the current state of knowledge about the mechanisms of protein trafficking between ER and Golgi. It describes what is known about the primary components of protein sorting machinery and trafficking, which are recognition sites on the proteins that are important for their interaction with the critical components of this machinery.

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COPI complex isoforms are required for the early acceptance of compatible pollen grains in Arabidopsis thaliana

Cited by 12 publications

References 50 publications

Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis

Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis

Dynamic Regulation of Lipid Droplet Biogenesis in Plant Cells and Proteins Involved in the Process

Critical Determinants in ER-Golgi Trafficking of Enzymes Involved in Glycosylation

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