Copper and ceruloplasmin activity in human amniotic fluid

Chan, Wai‐Yee; Richichi, Joann; Griesmann, Guy E.; Cushing, William J.; Kling, O.; Rennert, Owen M.

doi:10.1016/0002-9378(80)90244-6

Cited by 13 publications

(4 citation statements)

References 10 publications

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“…The data of Fleming and Gitlin (40) suggest that the fetal (and newborn) lung may also contribute ceruloplasmin to the blood plasma of the rat. In humans, the fetal circulation contains ceruloplasmin already well before birth (42,43), and there are traces of it in amniotic fluid (61,62), as we have now also shown for the rat. These various findings confirm the importance of ceruloplasmin in copper transport, not just for the normal mammalian adult, but also for the pregnant adult and the developing fetus, as it prepares for birth.…”

Section: Discussionmentioning

confidence: 56%

Ceruloplasmin and Copper Transport During the Latter Part of Gestation in the Rat

Lee¹,

Lancey²,

Montaser³

et al. 1993

Experimental Biology and Medicine

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We have examined the tissue uptake of 67Cu from ceruloplasmin versus albumin and transcuprein, after its intravenous administration to pregnant rats, in the last 4 days of gestation. 67Cu infused as in vivo-labeled ceruloplasmin remained on ceruloplasmin in the maternal circulation over the 4- to 6-hr time period examined, as determined by gel chromatography and immunoreactivity. That infused as in vitro-labeled serum was initially on transcuprein and albumin but soon also with new ceruloplasmin. On the basis of percent dose as well as total actual Cu transferred (taking into account the sizes of the two plasma Cu pools), ceruloplasmin was the preferred source of Cu for most tissues. Total uptake of Cu from ceruloplasmin was seven times greater than that from albumin and transcuprein for the placenta, whole fetus, and fetal liver. It was 2- to 6-fold greater for other tissues (except liver and kidney). When synthesis of maternal 67Cu-ceruloplasmin (from 67Cu administered on albumin and transcuprein) was inhibited with cycloheximide, uptake by nonhepatic tissues was reduced markedly. In the fetal circulation, entering 67Cu was initially associated with transcuprein and alpha-fetoprotein (or albumin), but then also appeared with ceruloplasmin. Specific receptors for ceruloplasmin were detected on membranes from the placenta as well as fetal liver; mRNA for ceruloplasmin was detected on the endoplasmic reticulum-bound polyribosomes of placenta/yolk sac, and of fetal and maternal liver. We conclude that Cu destined for the fetus is delivered mainly or exclusively by ceruloplasmin. It may enter via placental receptors, arriving in fetal plasma in ionic form, for later incorporation into fetal ceruloplasmin. The importance of ceruloplasmin as a source of plasma Cu for nonhepatic organs is also confirmed.

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Section: Discussionmentioning

confidence: 56%

Ceruloplasmin and Copper Transport During the Latter Part of Gestation in the Rat

Lee¹,

Lancey²,

Montaser³

et al. 1993

Experimental Biology and Medicine

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“…These, in turn, may be related to increases in the levels of copper-binding and zinc-binding proteins, such as ceruloplasmnin, superoxide dismutase, and metallothionein [31][32][33][34]. The amniotic fluid levels of these acute-phase reactants tend to increase during late pregnancy in response to increased oxidative stress, with the increases being particularly pronounced in obstetric disorders [35][36][37].…”

Section: Discussionmentioning

confidence: 99%

A Comparative Evaluation of Three Washing Procedures for Minimizing Exogenous Trace Element Contamination in Fetal Scalp Hair of Various Obstetric Outcomes

Razagui

2008

Biol Trace Elem Res

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A comparative evaluation of three washing methods for removing exogenous elemental contamination from fetal scalp hair is described. A total of 12 samples, including 6 from normal pregnancies and 6 from abruptio placentae (AP) cases, were used. Each was divided into four subsamples, with one left unwashed for comparison, while three were subjected to one of the three washing methods. The first consisted of agitating hair for 5 min in sequential and equal measures (30 ml) of acetone and water, followed by rinsing with acetone, and the process repeated twice. In the second and third, hair was agitated for 30 min in aqueous solutions of sodium lauryl sulfate (0.5%, w/v), and Triton X-100 (0.5%, v/v), respectively, followed by sequential rinsing with deionized water and acetone. After drying and microwave-assisted digestion in concentrated nitric acid, the subsamples' concentrations of zinc, copper, cadmium, lead, mercury and selenium were determined using inductively coupled mass spectrometry. All washed subsamples, irrespective of washing method used, had lower elemental concentrations than the corresponding, unwashed subsamples. These differences, taken as presumptive indicators of exogenous contamination, showed only small variations (<5%) between the three washing methods, irrespective of element, mode of delivery, and obstetric outcome. However, the contamination levels elicited by each of the three methods varied in magnitude according to individual elements, with those of copper being highest, followed by those of zinc, cadmium, mercury, selenium, and lead. They also varied according to obstetric outcome, being highest in the AP subsamples, while in the normal pregnancy group, copper and zinc contamination levels in the subsamples from neonates delivered by elective caesarean section were higher than in those from normal-delivery neonates. The data from this study suggest that the three washing methods used have comparable efficacies in removing exogenous elemental contamination from fetal hair, further inferring that it is probably a simple biological matrix to decontaminate. Possible contributory factors are discussed and contrasted with those affecting adult hair.

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“…The protein content of amniotic fluid reduced towards term. Ceruloplasmin demonstrated a major rise during 20–38 weeks of gestation, with a later decrease after 38 weeks [ 47 , 48 ]. Anagnostopoulos and co-workers reported that ceruloplasmin, alpha-1-antitrypsin, and zinc-alpha-2-glycoprotein are upregulated in specimens from pregnancies with Klinefelter syndrome fetuses, and were downregulated against proteins identified in specimens from normal fetuses [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Proteomic Assessment of Normal vs. Polyhydramnios Amniotic Fluid Based on Computational Analysis

et al. 2022

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Mass spectrometry-based proteomics have become a valued tool for conducting comprehensive analyses in amniotic fluid samples with pathologies. Our research interest is the finding and characterization of proteins related to normal vs. polyhydramnios (non-immune hydrops) pregnancy. Proteomic analysis was performed on proteins isolated from fresh amniotic fluid samples. Proteins were fractionated by 2DE using a different pI range (pI 3–11, pI 4–7) and analyzed with MALDI-TOF-MS. Furthermore, by using computational analysis, identified proteins in protein maps specific to normal vs. polyhydramnios pregnancy were compared and the quantities of expressed proteins were evaluated mathematically. Comparative analysis of proteome characteristic for the same polyhydramnios pregnancy fractionated by 2DE in different pI range (3–11 and 4–7) was performed and particular protein groups were evaluated for the quantification of changes within the same protein level. Proteins of normal and polyhydramnios pregnancies were fractionated by 2DE in pI range 3–11 and in pI range 4–7. Mass spectrometry analysis of proteins has revealed that the quantity changes of the main identified proteins in normal vs. polyhydramnios pregnancy could be assigned to immune response and inflammation proteins, cellular signaling and regulation proteins, metabolic proteins, etc. Specifically, we have identified and characterized proteins associated with heart function and circulatory system and proteins associated with abnormalities in prenatal medicine. The following are: serotransferrin, prothrombin, haptoglobin, transthyretin, alpha-1-antitrypsin, zinc-alpha-2-glycprotein, haptoglobin kininogen-1, hemopexin, clusterin, lumican, afamin, gelsolin. By using computational analysis, we demonstrated that some of these proteins increased a few times in pathological pregnancy. Computer assistance analysis of 2DE images suggested that, for the better isolation of the proteins’ isoforms, those levels increased/decreased in normal vs. polyhydramnios pregnancy, and the fractionation of proteins in pI rage 3–11 and 4–7 could be substantial. We analyzed and identified by MS proteins specific for normal and polyhydramnios pregnancies. Identified protein levels increased and/or modification changed in case of non-immune hydrops fetus and in cases of cardiovascular, anemia, growth restriction, and metabolic disorders. Computational analysis for proteomic characterization empower to estimate the quantitative changes of proteins specific for normal vs. polyhydramnios pregnancies.

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Copper and ceruloplasmin activity in human amniotic fluid

Cited by 13 publications

References 10 publications

Ceruloplasmin and Copper Transport During the Latter Part of Gestation in the Rat

Ceruloplasmin and Copper Transport During the Latter Part of Gestation in the Rat

A Comparative Evaluation of Three Washing Procedures for Minimizing Exogenous Trace Element Contamination in Fetal Scalp Hair of Various Obstetric Outcomes

Comparative Proteomic Assessment of Normal vs. Polyhydramnios Amniotic Fluid Based on Computational Analysis

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