Copper Binding by Lactic Acid Bacteria (LAB)

Mrvčić, Jasna; Stanzer, Damir; Bačun-Družina, Višnja; Stehlik-Tomas, Vesna

doi:10.12938/bifidus.28.1

Cited by 27 publications

(20 citation statements)

References 21 publications

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“…Several Lactobacillus strains have been reported to resist various metals ion at different concentration levels. Most studies emphasize on bioremediation or decontamination of heavy metals in the body and environmental perspectives [19,25,[42][43][44][45][46][47][48]. However, less is known about their capacity to tolerate with Zn 2+ and produce zinc nanomaterials simultaneously.…”

Section: Discussionmentioning

confidence: 99%

“…To our knowledge, we have reported a zinc-tolerant probiotic with the highest MTC value of 500 mM compared to other reports in the literature. In fact, the variations in resistance against metal ions between bacterial species are due to different properties of the bacteria, which include cell wall structure, functional groups and surface area [20,26,46].…”

Section: O-h Stretching and N-h Asymmetric Stretchingmentioning

confidence: 99%

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Sustainable microbial cell nanofactory for zinc oxide nanoparticles production by zinc-tolerant probiotic Lactobacillus plantarum strain TA4

2020

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Background: The use of microorganisms in the biosynthesis of zinc oxide nanoparticles (ZnO NPs) has recently emerged as an alternative to chemical and physical methods due to its low-cost and eco-friendly method. Several lactic acid bacteria (LAB) have developed mechanisms in tolerating Zn 2+ through prevention against their toxicity and the production of ZnO NPs. The LAB's main resistance mechanism to Zn 2+ is highly depended on the microorganisms' ability to interact with Zn 2+ either through biosorption or bioaccumulation processes. Besides the inadequate studies conducted on biosynthesis with the use of zinc-tolerant probiotics, the understanding regarding the mechanism involved in this process is not clear. Therefore, this study determines the features of probiotic LAB strain TA4 related to its resistance to Zn 2+. It also attempts to illustrate its potential in creating a sustainable microbial cell nanofactory of ZnO NPs. Results: A zinc-tolerant probiotic strain TA4, which was isolated from local fermented food, was selected based on the principal component analysis (PCA) with the highest score of probiotic attributes. Based on the 16S rRNA gene analysis, this strain was identified as Lactobacillus plantarum strain TA4, indicating its high resistance to Zn 2+ at a maximum tolerable concentration (MTC) value of 500 mM and its capability of producing ZnO NPs. The UV-visible spectroscopy analysis proved the formations of ZnO NPs through the notable absorption peak at 380 nm. It was also found from the dynamic light scattering (DLS) analysis that the Z-average particle size amounted to 124.2 nm with monodisperse ZnO NPs. Studies on scanning electron microscope (SEM), energy-dispersive X-ray (EDX) spectroscopy, and Fourier-transform infrared spectroscopy (FT-IR) revealed that the main mechanisms in ZnO NPs biosynthesis were facilitated by the Zn 2+ biosorption ability through the functional groups present on the cell surface of strain TA4. Conclusions: The strong ability of zinc-tolerant probiotic of L. plantarum strain TA4 to tolerate high Zn 2+ concentration and to produce ZnO NPs highlights the unique properties of these bacteria as a natural microbial cell nanofactory for a more sustainable and eco-friendly practice of ZnO NPs biosynthesis.

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Section: Discussionmentioning

confidence: 99%

Section: O-h Stretching and N-h Asymmetric Stretchingmentioning

confidence: 99%

Sustainable microbial cell nanofactory for zinc oxide nanoparticles production by zinc-tolerant probiotic Lactobacillus plantarum strain TA4

2020

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“…Previous reports have revealed that some lactobacilli, including Lactobacillus rhamnosus, L. plantarum, and L. brevis, can bind and remove heavy metals such as cadmium, lead, and copper in vitro (19,20). Besides the cadmium binding capacity, lactobacilli are also known to have antioxidative properties in human subjects, which may be another important characteristic for cadmium toxicity protection (21)(22)(23).…”

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confidence: 99%

Protective Effects of Lactobacillus plantarum CCFM8610 against Acute Cadmium Toxicity in Mice

Zhai

Wang

Zhao

et al. 2013

Appl Environ Microbiol

174

113

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bThis study evaluated the protective effects of Lactobacillus plantarum CCFM8610, a selected probiotic with good cadmium binding capacity, against acute cadmium toxicity in mice. Ninety mice were divided into prevention and therapy groups. In the prevention groups, CCFM8610 was administered at 10 9 CFU once daily for 7 days, followed by a single oral dose of cadmium chloride at 1.8 mg cadmium for each mouse. In the therapy groups, the same dose of CCFM8610 was administered for 2 days after an identical single dose of cadmium exposure. Mice that received neither cadmium nor culture or that received cadmium alone served as negative and positive controls, respectively. The effects of both living and dead CCFM8610 on cadmium ion concentrations in feces, liver, and kidney were determined. Moreover, the alterations in reduced glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and histopathology in the liver and kidney were investigated. The results showed that compared to the mice that received cadmium only, CCFM8610 treatment can effectively decrease intestinal cadmium absorption, reduce tissue cadmium accumulation, alleviate renal and hepatic oxidative stress, and ameliorate hepatic histopathological changes. Living CCFM8610 administered after cadmium exposure offered the most significant protection. Our results suggested that CCFM8610 is more effective against acute cadmium toxicity than a simple antioxidant treatment due to its special physiological functions and that it can be considered a new dietary therapeutic strategy against acute cadmium toxicity.

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“…As the pH of the solution increased, the binding capacity of the lactobacilli for Cu also increased ( Figure 2), probably due to a larger number of negatively charged carboxyl and phosphate groups on the cell surface [29]. Several authors have described a pH of 5.0 as optimal for the metal biosorption by lactobacilli [30,31]. Although at even higher pH values more negatively charged functional groups would be available, these conditions are remote and unsuitable, as they promote formation of insoluble copper(II) hydroxides and precipitates.…”

Section: Biosorptionmentioning

confidence: 99%

How to Deal with Uninvited Guests in Wine: Copper and Copper-containing Oxidases

Claus

2020

Fermentation

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Copper is one of the most frequently occurring heavy metals in must and wine. It is introduced by pesticides, brass fittings, and as copper sulphate for treatment of reductive off-flavors. At higher concentrations, copper has harmful effects on the wine. It contributes to the oxidation of wine ingredients, browning reactions, cloudiness, inhibition of microorganisms, and wine fermentation. Last but not least, there is also a danger to the consumer. At present, some physicochemical methods exist to reduce the copper content in must and wine, but they all have their shortcomings. A possible solution is the biosorption of metals by yeasts or lactobacilli. Copper can also reach must and wine in the form of copper-containing phenol oxidases (grape tyrosinase, Botrytis cinerea laccases). Similar to free copper, they oxidize phenolic wine compounds, and thus lead to considerable changes in color and nutritional value, making the product ultimately unsaleable. All measurements for enzyme inactivation such as heat treatment, and addition of sulphites or bentonite are either problematic or not effective enough. The application of oenological tannins could offer a way out but needs further research.

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Copper Binding by Lactic Acid Bacteria (LAB)

Cited by 27 publications

References 21 publications

Sustainable microbial cell nanofactory for zinc oxide nanoparticles production by zinc-tolerant probiotic Lactobacillus plantarum strain TA4

Sustainable microbial cell nanofactory for zinc oxide nanoparticles production by zinc-tolerant probiotic Lactobacillus plantarum strain TA4

Protective Effects of Lactobacillus plantarum CCFM8610 against Acute Cadmium Toxicity in Mice

How to Deal with Uninvited Guests in Wine: Copper and Copper-containing Oxidases

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