Copper-Induced Responses in Poplar Clones are Associated with Genotype- and Organ-Specific Changes in Peroxidase Activity and Proline, Polyamine, ABA, and IAA Levels

Kebert, Marko; Rapparini, Francesca; Neri, Luisa; Bertazza, Gianpaolo; Orlović, Saša; Biondi, Stefania

doi:10.1007/s00344-016-9626-x

Cited by 22 publications

(23 citation statements)

References 92 publications

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“…A highly reproducible extraction and purification procedure developed for leaf material of woody species was adapted from [4]. Phytohormones (IAA and ABA) were extracted from freeze-dried leaf samples with an extraction mixture (65:35, isopropanol: 0.2 M imidazole buffer, v/v, pH 7.0) and spiked with isotopically labeled internal standards [ 13 C 6 ] IAA and [ 2 H 4 ] ABA (OlChemIm Ltd., Olomouc, Czech Republic).…”

Section: Determination Of Leaf Indol-3-acetic Acid (Iaa) and Abscisic...mentioning

confidence: 99%

“…The methylated product was dried under a stream of N 2 and resuspended in ethyl acetate for analysis by gas chromatography coupled with mass spectrometry (GC-MS, model 7890A-5975C, Agilent Technologies, Santa Clara, CA, USA) in split-less mode [120,121]. Methyl esters of plant hormones were separated on an HP1 capillary column (60 m × 0.25 mm, 0.25 µm film thickness, Agilent, Santa Clara, CA, USA) using He as the carrier gas and temperature program as it was described in [4]. IAA and ABA were determined by using the Selected Ion Monitoring (SIM) mode, and final concentrations were calculated by calculating the ratios between corresponding peak and internal standard peak, peak area of m/z 130/136 and m/z 189/195 for IAA, and m/z 190/194 and 162/166 for ABA, according to the principles of isotope dilution [65,121].…”

Section: Determination Of Leaf Indol-3-acetic Acid (Iaa) and Abscisic...mentioning

confidence: 99%

“…The prime response of plants to environmental constraints such as drought, high light, salinity, heavy metals or extreme temperatures is a reduction in net CO 2 assimilation due to stomatal, mesophyll, and biochemical limitations [3][4][5][6]. This in turn results in an excess of light energy absorbed by chloroplasts relative to the capacity for photosynthesis and a consequent increase in the formation of reactive oxygen species (ROS, e.g., singlet oxygen, superoxide anion, hydrogen peroxide, and hydroxyl radicals [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

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Species-Level Differences in Osmoprotectants and Antioxidants Contribute to Stress Tolerance of Quercus robur L., and Q. cerris L. Seedlings under Water Deficit and High Temperatures

Kebert

Vuksanović

Stefels

et al. 2022

Plants

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The general aim of this work was to compare the leaf-level responses of different protective components to water deficit and high temperatures in Quercus cerris L. and Quercus robur L. Several biochemical components of the osmotic adjustment and antioxidant system were investigated together with changes in hormones. Q. cerris and Q. robur seedlings responded to water deficit and high temperatures by: (1) activating a different pattern of osmoregulation and antioxidant mechanisms depending on the species and on the nature of the stress; (2) upregulating the synthesis of a newly-explored osmoprotectant, dimethylsulphoniopropionate (DMSP); (3) trading-off between metabolites; and (4) modulating hormone levels. Under water deficit, Q. cerris had a higher antioxidant capacity compared to Q. robur, which showed a lower investment in the antioxidant system. In both species, exposure to high temperatures induced a strong osmoregulation capacity that appeared largely conferred by DMSP in Q. cerris and by glycine betaine in Q. robur. Collectively, the more stress-responsive compounds in each species were those present at a significant basal level in non-stress conditions. Our results were discussed in terms of pre-adaptation and stress-induced metabolic patterns as related to species-specific stress tolerance features.

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Section: Determination Of Leaf Indol-3-acetic Acid (Iaa) and Abscisic...mentioning

confidence: 99%

Section: Determination Of Leaf Indol-3-acetic Acid (Iaa) and Abscisic...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Species-Level Differences in Osmoprotectants and Antioxidants Contribute to Stress Tolerance of Quercus robur L., and Q. cerris L. Seedlings under Water Deficit and High Temperatures

Kebert

Vuksanović

Stefels

et al. 2022

Plants

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“…It was found in G. biloba seedlings that the proline content decreased significantly under low NaCl concentrations (50 and 100 mmol/L) and increased significantly under higher concentrations compared to non-treated controls [16]. Intriguingly, proline accumulation was strongly suppressed in tobacco leaves when the plants were exposed to a combination of different stresses [29].…”

Section: Introductionmentioning

confidence: 98%

“…Examples include synthesizing osmotic adjustment substances, such as proline, glycine betaine, polyols, polyamines and some soluble proteins, as well as protective enzymes [25][26][27]. Extensive literature about different salt and drought effects on accumulation of free proline in various vascular but also woody plant species is available [28][29][30][31][32][33]. An overall conclusion could be that proline accumulation has a significant impact on plant tolerance to salt since most of the studies reported considerably higher amounts of this amino acid in plants subjected to salt stress [34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Biochemical and Gene Expression Analyses in Different Poplar Clones: The Selection Tools for Afforestation of Halomorphic Environments

Galović

Kebert

Popović

et al. 2021

Forests

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Halomorphic soils cover a significant area in the Vojvodina region and represent ecological and economic challenges for agricultural and forestry sectors. In this study, four economically important Serbian poplar clones were compared according to their biochemical and transcriptomic responses towards mild and severe salt stress to select the most tolerant clones for afforestation of halomorphic soils. Three prospective clones of Populus deltoides (Bora-B229, Antonije-182/81 and PE19/66) and one of hybrid genetic background P. nigraxP. deltoides, e.g., P. x euramericana (Pannonia-M1) were hydroponically subjected to NaCl as a salt stress agent in a concentration range from 150 mM to 450 mM. Plant responses were measured at different time periods in the leaves. Biochemical response of poplar clones to salt stress was estimated by tracking several parameters such as different radical scavenging capacities (estimated by DPPH, FRAP and ABTS assays), accumulation of total phenolic content and flavonoids. Furthermore, accumulation of two osmolytes, glycine betaine and proline, were quantified. The genetic difference of those clones has been already shown by single nucleotide polymorphisms (SNPs) but this paper emphasized their differences regarding biochemical and transcriptomic salt stress responses. Five candidate genes, two putative poplar homologues of GRAS family TFs (PtGRAS17 and PtGRAS16), PtDREB2 of DREB family TFs and two abiotic stress-inducible genes (PtP5SC1, PtSOS1), were examined for their expression profiles. Results show that most salt stress-responsive genes were induced in clones M1 and PE19/66, thus showing they can tolerate salt environments with high concentrations and could be efficient in phytoremediation of salt environments. Clone M1 and PE19/66 has ABA-dependent mechanisms expressing the PtP5CS1 gene while clone 182/81 could regulate the expression of the same gene by ABA-independent pathway. To improve salt tolerance in poplar, two putative GRAS/SCL TFs and PtDREB2 gene seem to be promising candidates for genetic engineering of salt-tolerant poplar clones.

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Abscisic Acid and Plant Response Under Adverse Environmental Conditions

González‐Villagra

Figueroa

Luengo-Escobar

et al. 2021

Plant Performance Under Environmental Stress

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Copper-Induced Responses in Poplar Clones are Associated with Genotype- and Organ-Specific Changes in Peroxidase Activity and Proline, Polyamine, ABA, and IAA Levels

Cited by 22 publications

References 92 publications

Species-Level Differences in Osmoprotectants and Antioxidants Contribute to Stress Tolerance of Quercus robur L., and Q. cerris L. Seedlings under Water Deficit and High Temperatures

Species-Level Differences in Osmoprotectants and Antioxidants Contribute to Stress Tolerance of Quercus robur L., and Q. cerris L. Seedlings under Water Deficit and High Temperatures

Biochemical and Gene Expression Analyses in Different Poplar Clones: The Selection Tools for Afforestation of Halomorphic Environments

Abscisic Acid and Plant Response Under Adverse Environmental Conditions

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