Coral Diseases in Panjang Island, Java Sea: Diversity of Anti–Pathogenic Bacterial Coral Symbionts

Sabdono, Agus; Sawonua, Paiga Hanurin; Kartika, Ary Giri Dwi; Amelia, Jasmine Masyitha; Radjasa, Ocky Karna

doi:10.1016/j.proche.2015.03.004

Cited by 14 publications

(11 citation statements)

References 20 publications

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“…Kocurin, isolated from Kocuria palustris, was previously described as potent antibiotic compound against methicillin-resistant Staphylococcus aureus (MRSA) [50]. Another study of a bacterium with 96% sequence similarity to Kocuria flava S43 was able to inhibit bacteria causing coral disease, so called yellow blotch [65]. Detection of kocurin in extracts from this study indicated that it seems to be a common metabolite produced by bacteria of the genus Kocuria .…”

Section: Discussionmentioning

confidence: 79%

Biotechnological Potential of Bacteria Isolated from the Sea Cucumber Holothuria leucospilota and Stichopus vastus from Lampung, Indonesia

et al. 2019

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In order to minimize re-discovery of already known anti-infective compounds, we focused our screening approach on understudied, almost untapped marine environments including marine invertebrates and their associated bacteria. Therefore, two sea cucumber species, Holothuria leucospilota and Stichopus vastus, were collected from Lampung (Indonesia), and 127 bacterial strains were identified by partial 16S rRNA-gene sequencing analysis and compared with the NCBI database. In addition, the overall bacterial diversity from tissue samples of the sea cucumbers H. leucospilota and S. vastus was analyzed using the cultivation-independent Illumina MiSEQ analysis. Selected bacterial isolates were grown to high densities and the extracted biomass was tested against a selection of bacteria and fungi as well as the hepatitis C virus (HCV). Identification of putative bioactive bacterial-derived compounds were performed by analyzing the accurate mass of the precursor/parent ions (MS1) as well as product/daughter ions (MS2) using high resolution mass spectrometry (HRMS) analysis of all active fractions. With this attempt we were able to identify 23 putatively known and two previously unidentified precursor ions. Moreover, through 16S rRNA-gene sequencing we were able to identify putatively novel bacterial species from the phyla Actinobacteria, Proteobacteria and also Firmicutes. Our findings suggest that sea cucumbers like H. leucospilota and S. vastus are promising sources for the isolation of novel bacterial species that produce compounds with potentially high biotechnological potential.

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Section: Discussionmentioning

confidence: 79%

Biotechnological Potential of Bacteria Isolated from the Sea Cucumber Holothuria leucospilota and Stichopus vastus from Lampung, Indonesia

et al. 2019

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“…Species of Pseudoalteromonas , one of the most common bacterial genera in the marine environment, generally act as probiotics in coral (Moree et al, 2014;Muchlissin, Sabdono, & Permata, 2018;Rosado et al, 2019;Sabdono, Sawonua, Kartika, Amelia, & Radjasa, 2015;Shnit-Orland, Sivan, & Kushmaro, 2012), abalone (Offret, Jegou, Mounier, Fleury, & Le Chevalier, 2019;Offret, Rochard, et al, 2019), marine bivalves (Desriac et al, 2014;Rodrigues, Paillard, Dufour, & Bazire, 2015;Sun et al, 2016) shrimp (Amoah et al, 2019;Pham et al, 2014), lobster (Goulden, Hall, Pereg, Baillie, & Hoj, 2013), sea cucumbers (Chi et al, 2014;Zheng et al, 2012), fish (Mladineo et al, 2016;Sayes, Leyton, & Riquelme, 2016;Verner-Jeffreys, Shields, Bricknell, & Birkbeck, 2004), marine algae (Albakosh, Naidoo, Kirby, & Bauer, 2016;Nagel et al, 2012), and sea stars (Lloyd & Pespeni, 2018). Only a few reports have identifiedPseudoalteromonas species as pathogenic to marine organisms, including fish (Nelson & Ghiorse, 1999;Pujalte, Sitja-Bobadilla, Macian, Alvarez-Pellitero, & Garay, 2007), crabs (Talpur et al, 2011), algae (Goecke, Labes, Wiese, & Imhoff, 2013;Schroeder, Jaffer, & Coyne, 2003), and sea cucumbers (Liu et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

A novel screening method for the detection of Pseudoalteromonas shioyasakiensis, an emerging opportunistic pathogen that caused the mass mortality of juvenile Pacific abalone (Haliotis discus hannai) during a record-breaking heat wave

Li¹,

Wu²,

Wang³

et al. 2020

Preprint

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A serious disease was recorded in juvenile Pacific abalone in Fujian Province, China in 2018. Although this disease caused no obvious external lesions, affected abalone exhibited bleached pedal epithelial cells and a lack of attachment ability. Bacterial strains were collected and cultured from the mucus of moribund and healthy abalone. A novel method was developed for screening abalone pathogens, based on the important role of mucus in the innate immunity of marine organisms. Using bacterial isolation, sequence analysis, and experimental challenges in vitro and in vivo, we identified the bacterial strains pathogenic to abalone. We verified that abalone mortality rates were high when exposed to Pseudoalteromonas shioyasakiensis strain SDCH87 at high temperatures. This opportunistic pathogen had an outstanding growth ability in mucus, and disrupted first line mucosal immunity in the foot within three days. The unprecedented sea surface temperatures associated with the record-breaking 2018 heatwave in south China may have induced opportunistic pathogenic behavior in P. shioyasakiensis. To our knowledge, this is the first report to show that P. shioyasakiensis is a serious opportunistic pathogen of abalone, or possibly mollusks in general, in the context of a heatwave.

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“…This research used samples that were collected from Lovina, Bali and the National Park of Karimunjawa (5 47′21.6″S 110 3041.9″E) (March 2017), Jepara Indonesia by SCUBA diving 15-20 m in depth, at 18-25 C. The samples of nudibranchs were put into sterile plastic bags, filled with seawater and stored in a cool box (He et al, 2014;Sabdono et al, 2015), and immediately brought to the laboratory to be identified, to isolate and to purify the bacteria.…”

Section: Samplingmentioning

confidence: 99%

Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia

Kristiana

Bedoux

Pals

et al. 2020

PeerJ

Self Cite

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The aims of this work are to isolate bacterial symbionts from nudibranchs and subsequently to determine anti-Methicillin resistant Staphylococcus aureus (MRSA), cytotoxicity and anti-Herpes simplex virus type 1 (HSV-1) activities of bio compounds. A total of 15 species of nudibranchs were collected from Karimunjawa and five species from Bali, respectively. A total of 245 bacteria isolates were obtained. The anti-MRSA activity screening activity indicated two active bacteria. Ethyl acetate extracts from supernatants, indicating extracelullar compounds, showed an inhibition zone against MRSA at concentrations of 500-1,000 µg/ml. DNA sequence analysis showed that the strain KJB-07 from Phyllidia coelestis was closely related to Pseudoalteromonas rubra, whereas the strain NP31-01 isolated from Phyllidia varicosa was closely related to Virgibacillus salarius. The extract of Pseudoalteromonas rubra was cytotoxic to Vero cells at a concentration of 75 µg/ml. The extract of V. salarius presented no cytotoxicity at concentrations of 5-1,000 µg/ml. No anti HSV-1 was observed for both isolated bacteria. This is the first study describing research on anti-MRSA, cytotoxicity and anti HSV-1 activity of bacterial symbionts from the viscera of nudibranch. Compounds produced by Pseudoalteromonas rubra and V. salarius, had potential anti-MRSA activity. However, only extracts from Pseudoalteromonas rubra showed cytotoxic effects on Vero cells. Three compounds were identified by LC/MS after purification from culture supernatant. activity of marine bacteria associated with sponges from the waters off the coast of South East India. Microbiological Research 161 (3):252-262 DOI 10.1016/j.micres.2005.09.002. Avila C. 2006. Molluscan natural products as biological models: chemical ecology, histology, and laboratory culture. Chand S, Karuso P. 2017. Isolation and total synthesis of two novel metabolites from the fissurellid mollusc Scutus antipodes. Tetrahedron Letters 58(10):P. 1992. A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification. Research in Microbiology 143(8):785-790

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Coral Diseases in Panjang Island, Java Sea: Diversity of Anti–Pathogenic Bacterial Coral Symbionts

Cited by 14 publications

References 20 publications

Biotechnological Potential of Bacteria Isolated from the Sea Cucumber Holothuria leucospilota and Stichopus vastus from Lampung, Indonesia

Biotechnological Potential of Bacteria Isolated from the Sea Cucumber Holothuria leucospilota and Stichopus vastus from Lampung, Indonesia

A novel screening method for the detection of Pseudoalteromonas shioyasakiensis, an emerging opportunistic pathogen that caused the mass mortality of juvenile Pacific abalone (Haliotis discus hannai) during a record-breaking heat wave

Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia

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