Core Amino Acid Residues in the Morphology-Regulating Protein, Mms6, for Intracellular Magnetite Biomineralization

Yamagishi, Ayana; Narumiya, Kaori; Tanaka, Masayoshi; Matsunaga, Tadashi; Arakaki, Atsushi

doi:10.1038/srep35670

Cited by 23 publications

(23 citation statements)

References 55 publications

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“…The Mms6-MIC sequence contains seven negatively charged residues in total but the three residues Asp12, Glu13 and Asp19 showed the most significant changes during iron titration. Similar to the in vivo analysis, a single mutation on Asp12 and Glue13 showed a significant effect on magnetite crystal shape and size in MTB ( Yamagishi et al, 2016 ). One can thus assume that the other negative residues have some effect on ion binding and contribute to the negatively charged environment.…”

Section: Discussionsupporting

confidence: 61%

“…In summary, our results shed light on the residues that are involved in magnetite formation and their abilities to interact with specific ions. According to the literature, Mms6 is involved in magnetite nucleation, with deletion of mms6 or only its C-terminus causing changes in the size and shape of magnetite particles ( Tanaka et al, 2011 ; Kashyap et al, 2014 ; Yamagishi et al, 2016 ). In this study, in vitro iron co-precipitation by the Mms6-MIC had a minor effect on magnetite size and shape, which indicates that the major role of the protein is not realized during crystal growth.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, in vitro iron co-precipitation by the Mms6-MIC had a minor effect on magnetite size and shape, which indicates that the major role of the protein is not realized during crystal growth. It can be assumed that deletion of one of the MAP-encoding genes would initiate a chain reaction by interfering with the functions of other MAPs, ultimately leading to defects in particle size and shape ( Yamagishi et al, 2016 ). Experiments with the Mms7-MIC revealed similar results, with only a minor effect on crystal size and shape being seen, which may also indicate that the major role of this protein is seen during crystal nucleation and ion binding and less so during crystal growth.…”

Section: Discussionmentioning

confidence: 99%

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Understanding the Biomineralization Role of Magnetite-Interacting Components (MICs) From Magnetotactic Bacteria

et al. 2018

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Biomineralization is a process that takes place in all domains of life and which usually helps organisms to harden soft tissues by creating inorganic structures that facilitate their biological functions. It was shown that biominerals are under tight biological control via proteins that are involved in nucleation initiation and/or which act as structural skeletons. Magnetotactic bacteria (MTB) use iron biomineralization to create nano-magnetic particles in a specialized organelle, the magnetosome, to align to the geomagnetic field. A specific set of magnetite-associated proteins (MAPs) is involved in regulating magnetite nucleation, size, and shape. These MAPs are all predicted to contain specific 17–22 residue-long sequences involved in magnetite formation. To understand the mechanism of magnetite formation, we focused on three different MAPs, MamC, Mms6 and Mms7, and studied the predicted iron-binding sequences. Using nuclear magnetic resonance (NMR), we differentiated the recognition mode of each MAP based on ion specificity, affinity, and binding residues. The significance of critical residues in each peptide was evaluated by mutation followed by an iron co-precipitation assay. Among the peptides, MamC showed weak ion binding but created the most significant effect in enhancing magnetite particle size, indicating the potency in controlling magnetite particle shape and size. Alternatively, Mms6 and Mms7 had strong binding affinities but less effect in modulating magnetite particle size, representing their major role potentially in initiating nucleation by increasing local metal concentration. Overall, our results explain how different MAPs affect magnetite synthesis, interact with Fe2+ ions and which residues are important for the MAPs functions.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Understanding the Biomineralization Role of Magnetite-Interacting Components (MICs) From Magnetotactic Bacteria

et al. 2018

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“…As AgNPs with unique morphologies were also observed after long reaction times in this study, the difference in reaction kinetics seems to be a key factor for the formation of different sizes and morphologies of AgNPs, as discussed in a previous study on AuNP synthesis regulation [29][30][31]. In addition, further investigation of the interaction between peptides and specific crystal facets should be performed to elucidate the morphological regulation, because various biological molecules bound to a specific crystal surface are suggested to contribute to morphological regulation in the biomineralization process [32][33][34][35][36].…”

Section: Discussionsupporting

confidence: 62%

Array-Based Screening of Silver Nanoparticle Mineralization Peptides

Tanaka

Saito

Kita

et al. 2020

IJMS

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The use of biomolecules in nanomaterial synthesis has received increasing attention, because they can function as a medium to produce inorganic materials in ambient conditions. Short peptides are putative ligands that interact with metallic surfaces, as they have the potential to control the synthesis of nanoscale materials. Silver nanoparticle (AgNP) mineralization using peptides has been investigated; however, further comprehensive analysis must be carried out, because the design of peptide mediated-AgNP properties is still highly challenging. Herein, we employed an array comprising 200 spot synthesis-based peptides, which were previously isolated as gold nanoparticle (AuNP)-binding and/or mineralization peptides, and the AgNP mineralization activity of each peptide was broadly evaluated. Among 10 peptides showing the highest AgNP-synthesis activity (TOP10), nine showed the presence of EE and E[X]E (E: glutamic acid, and X: any amino acid), whereas none of these motifs were found in the WORST25 (25 peptides showing the lowest AgNP synthesis activity) peptides. The size and morphology of the particles synthesized by TOP3 peptides were dependent on their sequences. These results suggested not only that array-based techniques are effective for the peptide screening of AgNP mineralization, but also that AgNP mineralization regulated by peptides has the potential for the synthesis of AgNPs, with controlled morphology in environmentally friendly conditions.

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“…Mms6 is the most abundant and most studied MAP and, using mms6-knockout mutants, has been shown to be involved in magnetite formation in the magnetosome (Raschdorf et al, 2017). The deletion or mutation of mms6 caused defects in the size and shape of magnetite particles (Amemiya et al, 2007;Tanaka et al, 2011;Yamagishi et al, 2016). In vitro studies also showed that Mms6 affects magnetite particle size and shape during iron precipitation and that the 22 C-terminal residues are essential for function (Kashyap et al, 2014;Rawlings et al, 2016;Yamagishi et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

The importance of the helical structure of a MamC-derived magnetite-interacting peptide for its function in magnetite formation

Nudelman

González

Kolushiva

et al. 2018

Acta Cryst Sect D Struct Biol

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Biomineralization is the process of mineral formation by organisms and involves the uptake of ions from the environment in order to produce minerals, with the process generally being mediated by proteins. Most proteins that are involved in mineral interactions are predicted to contain disordered regions containing large numbers of negatively charged amino acids. Magnetotactic bacteria, which are used as a model system for iron biomineralization, are Gram-negative bacteria that can navigate through geomagnetic fields using a specific organelle, the magnetosome. Each organelle comprises a membrane-enveloped magnetic nanoparticle, magnetite, the formation of which is controlled by a specific set of proteins. One of the most abundant of these proteins is MamC, a small magnetosome-associated integral membrane protein that contains two transmembrane α-helices connected by an ∼21-amino-acid peptide. In vitro studies of this MamC peptide showed that it forms a helical structure that can interact with the magnetite surface and affect the size and shape of the growing crystal. Our results show that a disordered structure of the MamC magnetite-interacting component (MamC-MIC) abolishes its interaction with magnetite particles. Moreover, the size and shape of magnetite crystals grown in in vitro magnetite-precipitation experiments in the presence of this disordered peptide were different from the traits of crystals grown in the presence of other peptides or in the presence of the helical MIC. It is suggested that the helical structure of the MamC-MIC is important for its function during magnetite formation.

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Core Amino Acid Residues in the Morphology-Regulating Protein, Mms6, for Intracellular Magnetite Biomineralization

Cited by 23 publications

References 55 publications

Understanding the Biomineralization Role of Magnetite-Interacting Components (MICs) From Magnetotactic Bacteria

Understanding the Biomineralization Role of Magnetite-Interacting Components (MICs) From Magnetotactic Bacteria

Array-Based Screening of Silver Nanoparticle Mineralization Peptides

The importance of the helical structure of a MamC-derived magnetite-interacting peptide for its function in magnetite formation

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