Correction of glycaemia and GLUT1 level by mildronate in rat streptozotocin diabetes mellitus model

Sokolovska, Jeļizaveta; Isajevs, Sergejs; Sugoka, Olga; Sharipova, Jelena; Lauberte, Lasma; Svirina, Darja; Rostoka, Evita; Sjakste, Tatjana; Kalvinsh, Ivars; Sjakste, Nikolajs

doi:10.1002/cbf.1719

Cited by 23 publications

(22 citation statements)

References 46 publications

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“…The activation of PDGFR-α induces collagen deposition, fibrosis, and inflammatory responses in an infarcted myocardium [37]. Observed increased levels of Slc2a1 , the insulin-independent glucose transporter, indicate an adaptation of the myocardium to the diabetic environment for better glucose uptake and utilization [38]. …”

Section: Discussionmentioning

confidence: 99%

Partial deficiency of HIF-1α stimulates pathological cardiac changes in streptozotocin-induced diabetic mice

et al. 2014

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BackgroundDiabetic cardiomyopathy is associated with a number of functional and structural pathological changes such as left ventricular dysfunction, cardiac remodeling, and apoptosis. The primary cause of diabetic cardiomyopathy is hyperglycemia, the metabolic hallmark of diabetes. Recent studies have shown that a diabetic environment suppresses hypoxia-inducible factor (HIF)-1α protein stability and function. The aim of this study was to analyze the functional role of HIF-1α in the development of diabetic cardiomyopathy. We have hypothesized that the partial deficiency of HIF-1α may compromise cardiac responses under diabetic conditions and increase susceptibility to diabetic cardiomyopathy.MethodsDiabetes was induced by streptozotocin in wild type (Wt) and heterozygous Hif1a knock-out (Hif1a +/- ) mice. Echocardiographic evaluations of left ventricular functional parameters, expression analyses by qPCR and Western blot, and cardiac histopathology assessments were performed in age-matched groups, diabetic, and non-diabetic Wt and Hif1a +/- mice.ResultsFive weeks after diabetes was established, a significant decrease in left ventricle fractional shortening was detected in diabetic Hif1a +/- but not in diabetic Wt mice. The combination effects of the partial deficiency of Hif1a and diabetes affected the gene expression profile of the heart, including reduced vascular endothelial growth factor A (Vegfa) expression. Adverse cardiac remodeling in the diabetic Hif1a +/- heart was shown by molecular changes in the expression of structural molecules and components of the extracellular matrix.ConclusionsWe have shown a correlation between heterozygosity for Hif1α and adverse functional, molecular, and cellular changes associated with diabetic cardiomyopathy. Our results provide evidence that HIF-1α regulates early cardiac responses to diabetes, and that HIF-1α deregulation may influence the increased risk for diabetic cardiomyopathy.

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Section: Discussionmentioning

confidence: 99%

Partial deficiency of HIF-1α stimulates pathological cardiac changes in streptozotocin-induced diabetic mice

et al. 2014

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“…GLUT1 provides cells with glucose, but its abnormal expression also has a relationship with tumor growth and diabetes [9], [10], [34], [35]. We have shown that hypoxia and low sugar can induce GLUT1 expression and that the growth and metabolism of cancer cells expressing GLUT1 is rather higher than in other cells.…”

Section: Discussionmentioning

confidence: 99%

Functional Analyse of GLUT1 and GLUT12 in Glucose Uptake in Goat Mammary Gland Epithelial Cells

Zhu

Lin

et al. 2013

PLoS ONE

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Glucose transport, mediated by glucose transporters, is necessary for mammary gland development and lactation. GLUT1 and GLUT12 could both be expressed in the pregnant and lactating mammary gland to participate in the glucose uptake process. In this study, the goat GLUT1 and GLUT12 genes were cloned from Saanen dairy goats and transfected into goat mammary gland epithelial cells to assess their biological functions and distributions. The results showed that both goat GLUT1 and GLUT12 had 12 predicted membrane-spanning helices. Goat GLUT1 and GLUT12 each influenced the mRNA expression of the other transporter and increased the glucose consumption and lactose yield in GLUT1- and GLUT12-transfected goat mammary gland epithelial cells, respectively. The overexpression of GLUT1 or GLUT12 also increased the expression of amino acid transporters SLC1A5, SLC3A2 and SLC7A5 and affected genes expressions in GMGE cells. Using immunofluorescence staining, GLUT1 was detected throughout the cytoplasm and localized to the Golgi apparatus around the nuclear membrane, whereas GLUT12 was mainly distributed in the perinuclear region and cytoplasm. This study contributes to the understanding of how GLUT1 and GLUT12 cooperate in the incorporation of nutrient uptake into mammary gland epithelial cells and the promotion of milk synthesis in the goat mammary gland during lactation.

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“…Our high-sucrose diet may contribute to these differences. Indeed, a high-energy diet or a diabetes increases GLUT1 gene and protein expression in the brain, heart, kidney and testicles in rats (Rato et al, 2013;Sokolovska et al, 2011;Zhang et al, 2009a). This is the first report on brain MCT expression in aged rats.…”

Section: Energy Substrate Transporter Expressionmentioning

confidence: 92%

Long-term calorie restriction has minimal impact on brain metabolite and fatty acid profiles in aged rats on a Western-style diet

Roy

Hennebelle

St-Pierre

et al. 2013

Neurochemistry International

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Correction of glycaemia and GLUT1 level by mildronate in rat streptozotocin diabetes mellitus model

Cited by 23 publications

References 46 publications

Partial deficiency of HIF-1α stimulates pathological cardiac changes in streptozotocin-induced diabetic mice

Partial deficiency of HIF-1α stimulates pathological cardiac changes in streptozotocin-induced diabetic mice

Functional Analyse of GLUT1 and GLUT12 in Glucose Uptake in Goat Mammary Gland Epithelial Cells

Long-term calorie restriction has minimal impact on brain metabolite and fatty acid profiles in aged rats on a Western-style diet

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