Correlation Analysis of Nasopharyngeal Carcinoma TNM Staging with Serum EA IgA and VCA IgA in EBV and VEGF-C and -D

Abstract

BackgroundNasopharyngeal carcinoma often occurs in humans in the nasopharyngeal epithelium area. Ebstein-Barr (EB) virus plays a key role in the process of nasopharyngeal carcinoma lesions. Early antigen antibody (EA-IgA) and viral capsid antigen IgA (VCA-IgA) of EB virus detection in serum can effectively monitor the process of nasopharyngeal carcinoma lesions. Serum vascular endothelial growth factor (VEGF) -C and VEGF-D expression detection can reflect the distant metastases ability of human tumor cells.Material/Methods153 cases of nasopharyngeal carcinoma patients in our hospital were enrolled, while 148 cases of healthy adults were selected as control. ELISA was used to detect serum EA-IgA, VCA-IgA, VEGF-C and -D expression levels. Spearman rank correlation analysis was applied to test the correlation of nasopharyngeal carcinoma TNM clinical stage and different indexes.ResultsSerum EA-IgA, VCA-IgA, VEGF-C and -D expression in nasopharyngeal carcinoma patients was 43.74±2.6 U·mL−1, 62.5±2.7 U·mL−1, 473.25±3.4 pg·mL−1, and 498.36±2.3 pg·mL−1, respectively, which was significantly higher than in the control group as 18.65±3.7 U·mL−1, 23.74±1.5 U·mL−1, 225.42±2.3 pg·mL−1, and 257.24±3.5 pg·mL−1 (P<0.05). Nasopharyngeal carcinoma TNM clinical staging was obviously correlated with serum EA-IgA, VCA-IgA, and VEGF-C (P<0.05), but not VEGF-D (P>0.05).ConclusionsNasopharyngeal carcinoma patient serum EA-IgA and VCA-IgA expression levels were significantly correlated with TNM staging. The high levels of these 3 indicators suggest advanced nasopharyngeal carcinoma TNM staging and serious lesions.

Prognostic value of serum Epstein–Barr virus antibodies in patients with nasopharyngeal carcinoma and undetectable pretreatment Epstein–Barr virus DNA

“…With recent advances, several high‐quality, repeatable, and economical ELISA kits have been commercialized for testing serum EBV antibodies, including VCA‐IgA and EA‐IgA. Thus, the quantification of serum EBV antibodies in patients with NPC may reflect the tumor burden and provide reliable indexes for clinical TNM staging . However, to date, only two studies have evaluated the prognostic value of EBV antibodies in NPC .…”

Evaluation of Serum IgA Antibodies to Epstein-Barr Virus Early and Viral Capsid Antigens in Nasopharyngeal Carcinoma

“…[29] Activation of EBV results in a viral replication process (lytic process) when VCA and EA are expressed. [23,27] Therefore, morphological changes in epithelium such as metaplasia, insitu carcinoma and micro-invasive carcinomas that can be found in the early stages of NPC usually have not shown an increase of anti-EBV antibodies. [23] In addition, IgA levels are also influenced by the patient's immunological, racial/genetic and geographic conditions.…”

Hippo pathway contributes to cisplatin resistant-induced EMT in nasopharyngeal carcinoma cells

“…1 It has been reported that the annual incidence of NPC in Cantonese population in China to be over 20/100000, which counts for about 78% of the head and neck malignant tumors in recent years. 1,2 NPC is relatively uncommon in the United States with about 1.6 per 100,000 diagnosed in 2015. 3 The infection of Epstein-Barr virus is thought to be the main inducing factor for NPC.…”