Correlation between DNA of trichothecene-producing Fusarium species and deoxynivalenol concentrations in wheat-samples

Schnerr, Helge; Vogel, Rudi F.; Niessen, Ludwig

doi:10.1046/j.1472-765x.2002.01146.x

Cited by 69 publications

(47 citation statements)

References 18 publications

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“…Both tests are ideally suited for all analyses with high numbers of samples. Recently developed quantitative PCR techniques are too slow and expensive, although tight correlations between fungal DNA and DON concentrations in wheat grain have been reported (Schnerr et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Estimation of deoxynivalenol (DON) content by symptom rating and exoantigen content for resistance selection in wheat and triticale

et al. 2004

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Fusarium head blight (FHB) in wheat and triticale leads to contamination of the grain with the mycotoxin deoxynivalenol (DON) that is harmful to animal and man. A fast, low-cost, and reliable method for quantification of the DON content in the grain is essential for selection. We analysed 113 wheat and 55 triticale genotypes for their symptom development on spikes, Fusarium exoantigen (ExAg) and DON content in the grain after artificial inoculation with a highly aggressive isolate of F. culmorum in three (wheat) and six (triticale) location-by-year combinations. Additionally, in triticale the amount of Fusarium damaged kernels (FDK) was assessed. ExAg content was analysed by a newly developed Fusarium-specific plate-trapped antigen enzyme-linked immunosorbent assay (PTA-ELISA) and DON content by an immunoassay. A moderate disease severity resulted in an ExAg content of 0.87 optical density (OD) units in wheat and 1.02 OD in triticale. DON content ranged from 12.0 to 105.2 mg kg −1 in wheat and from 24.2 to 74.0 mg kg −1 in triticale. Genotypic and genotype-by-environment interaction variances were significant (P < 0.01). Coefficient of phenotypic correlation between DON content analysed by the immunoassay and ExAg content was r = 0.86 for wheat and r = 0.60 for triticale. The highest correlation between DON content and symptom rating was found by FHB rating in wheat (r = 0.77) and by FDK rating in triticale (r = 0.71). In conclusion, selection for reduced FHB symptoms should lead to a correlated selection response in low fungal biomass and low DON content in the grain.

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Section: Discussionmentioning

confidence: 99%

Estimation of deoxynivalenol (DON) content by symptom rating and exoantigen content for resistance selection in wheat and triticale

et al. 2004

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“…Uracil DNA glycosylase has no effect on native DNA, because native DNA does not contain uracil and in addition, the enzyme is inactivated by heat denaturation prior to PCR, in which deoxythymidin triphosphate (dTTP) has to be substituted by deoxyuracil triphosphate (dUTP). The developed quantitative LightCycler TM PCR-based assay was used to quantify the DNA from trichothecene-producing Fusarium species in 300 field inoculated wheat samples (46). The minimum detectable quantity of template DNA was 16 ng per gram of sample corresponding to 290 gene copies.…”

Section: Lightcycler Tm Pcrmentioning

confidence: 99%

The application of PCR in the detection of mycotoxigenic fungi in foods

Konietzny¹,

Greiner²

2003

Braz. J. Microbiol.

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It is estimated that 25 to 50% of the crops harvested worldwide are contaminated with mycotoxins. Because of the toxic and carcinogenic potential of mycotoxins, there is an urgent need to develop detection methods that are rapid and highly specific. The highly advanced physico-chemical methods for the analysis of mycotoxins in use, have the disadvantage that highly sophisticated clean-up and/or derivatization procedures must be applied. An alternative could be the detection of the mycotoxigenic moulds themselves, especially as molecular techniques have been introduced recently as powerful tools for detecting and identifying fungi. PCR methods for the detection of aflatoxigenic Aspergilli, patulin-producing Penicillum and trichothecene-as well as fumonisin-producing Fusaria strains have been described. The usefulness of the PCR methods developed so far to monitor quality and safety in the food an feed industry was already demonstrated. Thus, PCR may be applied to the screening of agricultural commodities for the absence of mycotoxin producers prior to or even after processing. Negative results in this assay indicate that a sample should be virtually free of mycotoxins. Only the positive samples left must be analyzed for the presence of mycotoxins using physicochemical standard methods. This review does not only summarize the so far developed qualitative and quantitative PCR assays for the detection of mycotoxigenic fungi in agricultural commodities, foods and animal feeds, but describes also strategies to develop new specific PCR assays for such a detection.

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“…Applications of SPR assay for detection of DON, fumonisins, aflatoxins, zearalenone and ochratoxin A have been www.intechopen.com developed and optimized (Daly et al, 2000;Schnerr et al, 2002;Tudos et al, 2003;van der Gaag et al, 2003). SPR sensitivity for aflatoxin B1 has been demonstrated to be higher than ELISA assay.…”

Section: Rapid Methods For Mycotoxin Analysismentioning

confidence: 99%

Rapid Methods as Analytical Tools for Food and Feed Contaminant Evaluation: Methodological Implications for Mycotoxin Analysis in Cereals

Cheli¹,

Campagnoli²,

Pinotti³

et al. 2012

Food Production - Approaches, Challenges and Tasks

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Correlation between DNA of trichothecene-producing Fusarium species and deoxynivalenol concentrations in wheat-samples

Cited by 69 publications

References 18 publications

Estimation of deoxynivalenol (DON) content by symptom rating and exoantigen content for resistance selection in wheat and triticale

Estimation of deoxynivalenol (DON) content by symptom rating and exoantigen content for resistance selection in wheat and triticale

The application of PCR in the detection of mycotoxigenic fungi in foods

Rapid Methods as Analytical Tools for Food and Feed Contaminant Evaluation: Methodological Implications for Mycotoxin Analysis in Cereals

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