This work reports on the evolution law of helium bubbles in Hastelloy N alloy on post-irradiation annealing conditions. After helium ion irradiation at room temperature and subsequent annealing at 600 °C (1 h), the transmission electron microscopy (TEM) micrograph indicates the presence of helium bubbles with size of 2 nm in the depth range of 0–300 nm. As for the sample further annealed at 850 °C (5 h), on one hand, a “Denuded Zone” (0–38 nm) with rare helium bubbles forms due to the decreased helium concentration. On the other hand, the “Ripening Zone” (38–108 nm) and “Coalescence Zone” (108–350 nm) with huge differences in size and separation of helium bubbles, caused by different coarsening rates, are observed. The mechanisms of “Ostwald ripening” and “migration and coalescence”, experimentally proved in this work, may explain these observations.