Correlation between sperm motility and sperm chromatin/DNA damage before and after cryopreservation and the effect of folic acid and nicotinic acid on post-thaw sperm quality in normozoospermic men

Rarani, Fahimeh Zamani; Golshan-Iranpour, farhad; Dashti, Gholamreza

doi:10.1007/s10561-019-09775-6

Cited by 21 publications

(16 citation statements)

References 30 publications

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“…Sperm cryopreservation has often been used for preservation of fertility ability before factors such as cytotoxic radiotherapy and chemotherapy, cytotoxic occupational exposure, certain diseases, and surgeries could lead ejaculatory and testicular failures [4,5]. Finally, it is widely used to avoid second percutaneous epididymal sperm aspiration or testicular sperm extraction [6].…”

Section: Introductionmentioning

confidence: 99%

In vitro application of Ceratonia siliqua improved sperm parameters and chromatin quality after vitrifacation in normozoospermic aged men

Faramarzi

Aghaz

Bakhtiari

et al. 2019

Middle East Fertil Soc J

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Background Vitrification is the main technique in the assisted reproductive technique (ART) labs. Sperm vitrification exposes sperm to damage. The aim of the present study was to evaluate the in vitro effect of Ceratonia siliqua (C. siliqua) application on sperm parameters and chromatin quality in normozoospermic aged men. Semen samples (n = 40) were collected from normozoospermic men over 45 years old. Each specimen was divided into four aliquots to form the subsequent groups: fresh (group I), vitrification without treatment (group II), vitrification with the medium supplemented by 20 μg/ml C. siliqua (group III), and vitrification with the thawing medium supplemented by 20 μg/ml C. siliqua (group IV). Sperm progressive motility, normal morphology and viability were assessed. Also, sperm chromatin quality was evaluated by aniline blue (AB), toluidine blue (TB), and sperm chromatin dispersion (SCD) staining. Results Vitrification caused a significant decrease in sperm progressive motility, normal morphology and viability as well as chromatin quality compared to fresh samples (p < 0.05). Supplementation of vitrification/thawing medium with C. siliqua significantly improved sperm progressive motility, normal morphology, viability, and chromatin quality compared to vitrification without any supplementation (p < 0.05). Conclusions The study showed that C. siliqua can improve the detrimental effect of vitrification on sperm parameters and chromatin quality of normozoospermic aged men.

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Section: Introductionmentioning

confidence: 99%

In vitro application of Ceratonia siliqua improved sperm parameters and chromatin quality after vitrifacation in normozoospermic aged men

Faramarzi

Aghaz

Bakhtiari

et al. 2019

Middle East Fertil Soc J

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“…In order to assess sperm DNA integrity, the acridine orange (AO) staining method was employed (Hoshi, Katayose, Yanagid, Kimura, & Sato, ; Rarani, Golshan‐Iranpour, & Dashti, ; Tejada, Mitchell, Norman, Marik, & Friedman, ). AO staining was carried out following the procedure described by Tejada et al ().…”

Section: Methodsmentioning

confidence: 99%

“…After 5 min, smears were washed with distilled water, covered with a coverslip and sealed with nail polish to protect the smear from drying. The smears were examined using a fluorescence microscope (Olympus, Tokyo, Japan) with the following filter combination: 450–490 nm excitation, 510‐nm reflector and 520‐nm barrier filter (Rarani et al, ).…”

Section: Methodsmentioning

confidence: 99%

Effects of temperature and storage time on the motility, viability, DNA integrity and apoptosis of processed human spermatozoa

et al. 2019

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The aim of this study was to evaluate motility, viability, DNA integrity and apoptosis of spermatozoa when washed semen samples were kept for up to 12 days at 4–6°C and 25°C. In this experimental study, 26 normozoospermic semen samples were washed twice in Modified Ham's F10 and resuspended in IVF fertilisation medium. Half of the specimens were stored at 4–6°C, and the other half was kept at 25°C for 12 days. The proportions of viable, motile, spermatozoa with double‐stranded DNA and apoptotic spermatozoa were examined during storage time. Apoptosis was measured using annexin V‐PI staining followed by flow cytometry. Results showed that sperm motility and viability decreased during 12 days of sample storage (p < .001). There was no significant difference between the two temperatures in terms of motility and viability for up to 2 days (p < .05). The percentage of spermatozoa with double‐stranded DNA remained unchanged during the 12 days of storage at both temperatures (p > .05). Although there was no difference between the two temperatures in terms of motility, viability and apoptosis during the first two days of storage, storage of spermatozoa at 4–6°C is better than storage for a longer period than storage at 25°C. Sperm DNA resisted against denaturation during storage.

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“…Sperm head shape and size are not only affected by cryopreservation, but their movement is also disrupted. Cryopreservation exerts oxidative stress on sperm, causing cryodamage and reducing progressive motility in humans [ 44 ]. Cryopreservation affects the composition of sperm’s membrane lipids, survival rate, motility and damages DNA in humans [ 44 ].…”

Section: Effect On Sperm Motilitymentioning

confidence: 99%

Effect of Sperm Cryopreservation in Farm Animals Using Nanotechnology

Akhtar

et al. 2022

Animals

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Sperm cryopreservation is one of the sublime biotechnologies for assisted reproduction. In recent decades, there has been an increasing trend in the use of preserved semen. Post-thaw semen quality and values vary among animals of the same species. Similarly, there are species-specific variations in sperm morphology, i.e., sperm head, kinetic properties, plasma membrane integrity, and freezability. Similarly, the viability of sperm varies in the female reproductive tract, i.e., from a few hours (in cattle) to several days (in chicken). Various steps of sperm cryopreservation, i.e., male health examination, semen collection, dilution, semen centrifugation, pre- and post-thaw semen quality evaluation, lack standardized methodology, that result in differences in opinions. Assisted reproductive technologies (ART), including sperm preservation, are not applied to the same extent in commercial poultry species as in mammalian species for management and economic reasons. Sperm preservation requires a reduction in physiological metabolism by extending the viable duration of the gametes. Physiologically and morphologically, spermatozoa are unique in structure and function to deliver paternal DNA and activate oocytes after fertilization. Variations in semen and sperm composition account for better handling of semen, which can aid in improved fertility. This review aims to provide an update on sperm cryopreservation in farm animals.

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Correlation between sperm motility and sperm chromatin/DNA damage before and after cryopreservation and the effect of folic acid and nicotinic acid on post-thaw sperm quality in normozoospermic men

Cited by 21 publications

References 30 publications

In vitro application of Ceratonia siliqua improved sperm parameters and chromatin quality after vitrifacation in normozoospermic aged men

In vitro application of Ceratonia siliqua improved sperm parameters and chromatin quality after vitrifacation in normozoospermic aged men

Effects of temperature and storage time on the motility, viability, DNA integrity and apoptosis of processed human spermatozoa

Effect of Sperm Cryopreservation in Farm Animals Using Nanotechnology

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