Correlation between systemic inflammatory response and quality of life in patients with chronic rhinosinusitis

Abstract

Background Local sinonasal inflammation resulting from altered T‐cell immune signaling is a contributor to the pathogenesis of chronic rhinosinusitis (CRS). CRS patients experience negative impacts on quality of life (QOL) and suffer from comorbidities linked to systemic inflammation. However, systemic inflammatory profiling to evaluate the association between systemic inflammation and QOL in CRS has not been performed. Our objectives were to compare local and systemic inflammatory gene expression in patients with CRS to determine if systemic markers of inflammation associate with disease severity and disease‐specific QOL. Methods A prospective observational study was conducted comparing 16 patients with CRS to 10 controls. Inflammatory gene expression in the anterior ethmoid tissues and peripheral blood of patients was measured using multiplex gene expression analysis and correlated to disease severity (computed tomography and nasal endoscopy) and disease‐specific QOL (22‐item Sino‐Nasal Outcome Test [SNOT‐22] and Rhinosinusitis Disability Index) using linear regression analyses. Results Patients with CRS showed significant increases in the expression of ctla4 and jak1 in sinonasal tissue and blood (p < 0.05), whereas the gene expression of hla‐dqa1, hla‐dqb1, and dusp4 was significantly decreased in patients with CRS compared to controls (p < 0.05). Soluble and local ctla4 and jak1 showed a significant positive correlation with clinical markers of disease severity and disease‐specific QOL (p < 0.05). Conclusion Local and systemic gene expression involved in T‐cell immune signaling was found to be significantly altered in the blood and sinonasal tissues of patients with CRS compared to controls and significantly correlated to disease severity and QOL in patients with CRS.

Focusing on quality of life in chronic rhinosinusitis patients

“…Both studies found that distinct biomarkers were associated with disease‐specific and/or general quality of life scores. Orb et al . additionally found that local and systemic inflammatory gene expression correlates with clinical markers of disease severity.…”

“…Chowdhury et al . and Orb et al . assessed mucus cytokines and expression of genes involved in T‐cell immune signaling, respectively.…”

Evaluation of the incidence of inflammatory and tumor pathology of nose and nasal sinus region

“…One explanation would be the fact that with age there is an immune vulnerability due to various comorbidities, and on the other hand there is a cumulative effect of inflammatory, infectious or non-infectious phenomena over time. Regarding the gender difference, it is probably due to the high prevalence of smokers over female smokers [ 10 ].…”

Chemokine CCL19 and Its Receptors CCR7 and CCRL1 in Chronic Rhinosinusitis

“…Tissue specimens were either immediately frozen and stored at −80°C until being subjected to mRNA extraction and gene expression analysis, or fixed in 4% neutral formalin for 48 hours until being subjected to tissue processing by the Molecular Diagnostics Core at the Huntsman Cancer Institute (Salt Lake City, UT) and protocols for immunohistochemical analysis, as previously detailed. 29 , 30 …”

“…mRNA extraction and multiplex gene expression analysis were performed as previously reported. 29 , 30 Briefly, after being thawed at 4°C, ethmoid tissues were placed in 0.6 mL of lysis buffer containing 5-mm stainless-steel beads and homogenized using a Qiagen Tissuelyser LT (Qiagen; Hilden, Germany). Nucleic acid was extracted using a Qiagen AllPrep RNA microRNA Universal Kit and a QiaCube, following the manufacturer’s instructions.…”