Correlation between total phenolic and flavonoid contents with antioxidant activity of Malaysian stingless bee propolis extract

Asem, Nornaimah; Gapar, Nur Adilah Abdul; Hapit, Nor Hussaini Abd; Omar, Eshaifol Azam

doi:10.1080/00218839.2019.1684050

Cited by 71 publications

(53 citation statements)

References 26 publications

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“…In addition, the IC 50 of DPPH assay correlated negatively with the total phenolic and TAC (r � −0.744 and r � −0.711, respectively) (Table 3). e same correlation was mentioned in other studies [20,43,49]. Antioxidant activities were also assessed by the ABTS and FRAP assays (Table 3).…”

Section: Antioxidant Capacities Of Propolis Samplesmentioning

confidence: 55%

“…e antioxidant activity 4 Journal of Food Quality and the ability of samples to scavenge free radicals were evaluated by three methods, and the results are expressed as IC 50 mg/mL (Table 4). Large differences were found in their antioxidant activity, which are related to their chemical composition, especially, the total phenolic and flavonoids contents [17,48,49]. e results showed a strong free radical scavenging by DPPH assay with IC 50 ranging between 0.02 ± 0.001 mg/mL in sample S6 from Al-Khalil and 1.13 ± 0.054 mg/mL in sample S8 from Ramallah.…”

Section: Antioxidant Capacities Of Propolis Samplesmentioning

confidence: 92%

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In Vitro Evaluation of Palestinian Propolis as a Natural Product with Antioxidant Properties and Antimicrobial Activity against Multidrug-Resistant Clinical Isolates

Daraghmeh

Imtara

2020

Journal of Food Quality

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The present work reveals, for the first time, the antioxidant and antibacterial properties of propolis samples collected from different regions of Palestine. The content on bioactive compounds has been estimated by total phenolic and flavone and flavonol content, while their antioxidant activity has been determined by radical scavenging methods of 1,2-diphenyl-1-picrylhydrazyl radical (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical (ABTS), and ferric reducing power assay (FRAP). The disc diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods were carried out to evaluate the antibacterial activity of Palestinian propolis against multidrug-resistant clinical isolates, including Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus faecalis. The results showed that the total phenolic content ranged from 9.62 mg to 124.94 mg gallic acid equivalent GAE/g of propolis, and the flavone and flavonol content ranged from 1.06 to 75.31 mg quercetin equivalent QE/g of propolis. The samples S6 from Al-Khalil presented the strongest radical scavenging activity toward DPPH, ABTS free radicals, and FRAP assay with IC50 values of 0.02, 0.03, and 0.05 mg/mL, respectively. The results of antibacterial activity indicated that the propolis samples inhibit the growth of Gram-positive strains better than Gram-negative ones. In addition, a strong correlation was observed between the pH, resin, balsam, total phenolic, flavones and flavonol, and total antioxidant capacity (TAC) from one side and the antibacterial activity of propolis samples except on Pseudomonas aeruginosa.

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Section: Antioxidant Capacities Of Propolis Samplesmentioning

confidence: 55%

Section: Antioxidant Capacities Of Propolis Samplesmentioning

confidence: 92%

In Vitro Evaluation of Palestinian Propolis as a Natural Product with Antioxidant Properties and Antimicrobial Activity against Multidrug-Resistant Clinical Isolates

Daraghmeh

Imtara

2020

Journal of Food Quality

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“…Rosli et al [ 8 ], Gapar [ 15 ], and Asem et al [ 16 ] have investigated the antioxidant activity of propolis extract from T. apicalis via 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 2′-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS + ) assay. Gapar [ 15 ] also correlated the antioxidant activity of T. apicalis propolis extract with total phenolic and flavonoid content.…”

Section: Introductionmentioning

confidence: 99%

GC‐MS Evaluation, Antioxidant Content, and Cytotoxic Activity of Propolis Extract from Peninsular Malaysian Stingless Bees, Tetrigona Apicalis

Mohamed

Ismail

Omar

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Introduction. Propolis has been used traditionally in several countries for treating various diseases as it possessed healing properties including antioxidant and anticancer qualities. In Peninsular Malaysia, Tetrigona apicalis is one of the species of stingless bees mainly found in virgin jungle reserves which largely contribute to propolis production. Therefore, this study is designed to evaluate the phytochemical contents, antioxidant properties, and the cytotoxic effect of ethanolic crude of propolis extract against MCF7 and MCF 10A cell lines. Method. The ethanolic extract of propolis (EEP) was extracted using 80% ethanol. Identification of phytochemical contents and antioxidant properties of EEP was analysed by gas chromatography-mass spectrometry (GC-MS) and using 2, 2′-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) method, respectively. The EEP cytotoxic activity was evaluated on MCF7 and MCF 10A using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Results. Phytochemical contents of EEP demonstrated 28 compounds in which caryophyllene (99%), β-amyrin (96%), α-amyrin (93%), and caryophyllene oxide (93%) were the main compounds. The percentage of ABTS+ scavenging activity of EEP showed an inhibition of 9.5% with half-inhibitory concentration (IC50) value of 1.68 mg/mL. The EEP reduced MCF7 cells viability at IC50 value of 62.24 μg/mL, 44.15 μg/mL, and 32.70 μg/mL at 24, 48, and 72 hours, respectively. The IC50 value of MCF 10A was 49.55 μg/mL, 56.05 μg/mL, and 72.10 μg/mL at 24, 48, and 72 hours, respectively. The EEP cytotoxic effect of T. apicalis was more selective towards MCF7 at 72-hour incubation with a selectivity index (SI) of 2.20. Conclusion. The EEP has been shown to have antioxidants and potential bioactive compounds and inhibited proliferation of the MCF7 cells. Further studies on the EEP role in the apoptosis pathway and its screening towards other cell lines will be evaluated.

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“…Hasil mengindikasikan senyawa flavonoid (flavonoid total) dalam biji dan daun kacang hijau memiliki kontribusi 96% dari potensi antioksidannya. Temuan ini sejalan dengan penelitianpenelitian sebelumnya yang juga menyatakan ada hubungan yang linear antara kadar senyawa flavonoid dengan potensi antioksidannya 46,53 .…”

Section: Uji Potensi Antioksidanunclassified

Potensi Antioksidan Biji dan Daun Kacang Hijau (Vigna radiate L.) dan Studi Korelasinya Dengan Kadar Flavonoid Total

Fakhrudin¹,

Kurniailla²,

Fatimah³

2020

J. Penelit Pascapanen Pertan

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Biji dan daun kacang hijau (KH) mengandung senyawa flavonoid sebagai antioksidan yang bermanfaat bagi kesehatan. KH dapat diekstrasi dengan air dan etanol menghasilkan ekstrak kaya flavonoid untuk bahan baku produk suplemen kesehatan dan obat tradisional. Penelitian bertujuan membandingkan potensi antioksidan dari biji dan daun KH, menganalisis kandungan flavonoid dan korelasinya dengan potensi antioksidan. Biji dan daun KH diekstraksi dengan air dan etanol masing-masing menggunakan metode dekokta dan maserasi. Potensi antioksidan ekstrak dievaluasi dengan metode FRAP menggunakan trolox sebagai pembanding, dan nilainya dinyatakan sebagai ekuivalensi terhadap trolox per gram ekstrak. Kadar flavonoid total ditetapkan secara spektrofotometri dengan rutin sebagai pembanding. Analisis kandungan flavonoid ekstrak dilakukan dengan kromatografi lapis tipis. Ekstraksi air biji KH memiliki potensi antioksidan 69,65±10,89 µmol TE/g, lebih tinggi dari daunnya 40,52±3,33µmol TE/g. Pada ekstraksi dengan etanol, potensi antioksidan biji KH lebih besar daripada daunnya (131,50±12,77µmol TE/g dibanding 9,37±0,42 µmol TE/g). Ekstrak air biji dan daun KH masing-masing memiliki kadar flavonoid total 2,98±0,03 dan 2,64±0,29 %, sedangkan ekstrak etanolnya masing- masing sebesar 4,71±0,72 dan 1,06±0,16 %. Kadar flavonoid ekstrak sebanding dengan potensi antioksidannya. Vitexin dan rutin merupakan senyawa flavonoid utama dalam biji dan daun KH. Daun KH juga memiliki potensi antioksidan yang cukup kuat dan kaya akan senyawa flavonoid. Antioxidant Potency of Mung Bean (Vigna radiate L) Seed and Leaf and Its Correlation with The Total Flavonoid Content.Mug Bean (MB) seeds dan leaves contain flavonoids with promising antioxidant activity. MB can be extracted with water and ethanol to yield flavonoid-enriched extract for health supplement and traditional medicine products raw material. This study aimed to compare the antioxidant potency of MB seed and leaf, and to analyze the flavonoid content and its correlation with antioxidant potency. MB seed and leaf were extracted with water and ethanol using decocta and maceration, respectively. The antioxidant potency of the extracts was evaluated by the FRAP method using Trolox as a reference control. The antioxidant potency was expressed as Trolox equivalent per gram of extract. The total flavonoid contents were determined spectrophotometrically with Rutin as a reference compound. Analysis of flavonoid component in the extracts was done by thin layer chromatography. The water extracts of MB seeds and leaves demonstrated antioxidant potency of 69.65±10.89 and 40.52±3.33 µm TE/g, whereas the ethanol extracts were 131.50±12.77 and 9.37±0.42 µmol TE/g, respectively. The water extracts of MB seeds and leaves have total flavonoid content of 2.98±0.03 and 2.64±0.29 %, whereas the ethanol extracts were 4.71±0.72 and 1.06±0.16 %, respectively. We found that the total flavonoid content is proportional with the antioxidant potency. Vitexin and Rutin are the main flavonoids in MB. MB leaves also showed a powerful antioxidant potency and are rich of flavonoids.

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Correlation between total phenolic and flavonoid contents with antioxidant activity of Malaysian stingless bee propolis extract

Cited by 71 publications

References 26 publications

In Vitro Evaluation of Palestinian Propolis as a Natural Product with Antioxidant Properties and Antimicrobial Activity against Multidrug-Resistant Clinical Isolates

In Vitro Evaluation of Palestinian Propolis as a Natural Product with Antioxidant Properties and Antimicrobial Activity against Multidrug-Resistant Clinical Isolates

GC‐MS Evaluation, Antioxidant Content, and Cytotoxic Activity of Propolis Extract from Peninsular Malaysian Stingless Bees, Tetrigona Apicalis

Potensi Antioksidan Biji dan Daun Kacang Hijau (Vigna radiate L.) dan Studi Korelasinya Dengan Kadar Flavonoid Total

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