Correlation of Free Hemoglobin Level and Plasma Nitric Oxide in Packed Red Cell During Blood Bank Storage Period

Abstract

Stored red blood cells undergo morphological and biochemical changes with increased storage time, commonly refferred as the storage lesion, reduced integrity of erythrocyte membrane, causing hemolysis and increased free plasma hemoglobin level. Nitric Oxide (NO) is an endogenous vasodilator with the major role in vascular and blood flow regulation. Accumulation of free hemoglobin (fHb) during storage RBC hemolysis is thought to be correlated with elevated NO consuming causes low NO level that significantly impair endothelial function. This study aim to was to analyze the correlation of free Hb and plasma nitric oxide level during PRC storage period in the blood bank. This research was an analytic study with a cross-sectional design to 14 unit PRC those were stored in Dr. M. Djamil Padang Hospital Blood Bank. The study was conducted from May 2016 to August 2017. The assay was performed for 28 days of storage with one week intervals. Free plasma hemoglobin and NO level were analyzed by cyanmethemoglobin and colorimetry method respectively. Spearman test was used to access the correlation between free Hb level and nitric oxide plasma with p<0.05 means significant. Most donors were male (85.7%) with age range were 33(9) years. Free Hb and nitric oxide level were significantly differenced in each week storage periods (p<0.05). Two parameters was significantly had moderate negative correlation during storage periods (r=-0.56; p=0.01).

Analysis of Packed Red Cell (PCR) storage time against changes in routine blood values in the blood donor unit of Indonesian Red Cross Society Medan City

“…Fitria's study showed a significant increase in haemoglobin for 28 days. first, the seventh day, fourteenth day, twenty-first day, and twentyeighth day [11]. In other cases hemolysis were found of the red cells increases due to processing and during storage and is maximum during the first week.…”

Pengaruh Lama Simpan Dan Konsentrasi Manitol Pada Packed Red Cell Sagm Terhadap Kadar Hemoglobin Dan Hematokrit

“…2 Penelitian tahun 2019 oleh Fitria et al menunjukkan hasil peningkatan kadar hemoglobin pada PRC yang disimpan selama 28 hari, yaitu 0,03 g/dL pada hari penyimpanan ke-0 dan 0,26 g/dL pada hari penyimpanan ke-28. 3 Penyimpanan PRC dapat mempengaruhi nilai hematokrit. Hematokrit atau volume sel darah merah terkompresi dalam darah yang disimpan dapat meningkat karena perubahan morfologi eritrosit selama penyimpanan.…”