Correlation of leukocytospermia with clinical infection and the positive effect of antiinflammatory treatment on semen quality

Lackner, Jakob; Herwig, Ralf; Schmidbauer, Joerg; Schatzl, Georg; Kratzik, Christian; Marberger, Michael

doi:10.1016/j.fertnstert.2006.01.032

Cited by 88 publications

(59 citation statements)

References 31 publications

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“…The presence of leukocytes in semen appears to be the factor enhancing sperm lipid membrane peroxidation. However, leukocytospermia and bacteriospermia are reported as independent observations in infertile men, particularly if no clinical sign of infection is present [Lackner et al 2006].…”

Section: Introductionmentioning

confidence: 99%

Lipid Peroxidation in Human Spermatozoa from Men with Genitourinary Infections

Cosci

Moretti

Collodel

2008

Systems Biology in Reproductive Medicine

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A fluorescent assay was used to reveal the presence of lipid peroxidation (LPO) in spermatozoa from individuals with genitourinary infections (GI). The incidence of LPO was compared with sperm pathologies (apoptosis, immaturity, necrosis) evaluated by transmission electron microscopy (TEM) and motility. A C11-BODIPY 581/591 probe was used to detect and localize LPO in sperm from 34 individuals. The sperm morphological characteristics were studied by TEM and the results were mathematically assessed. Using the LPO percentage we divided the patients into four groups. Group 1 included ten patients with GI with almost normal progressive motility, a very low percentage of LPO and sperm pathology values that were not far from the standard ranges. Group 2 had eleven infected patients showing reduced motility, LPO of 10 to 20% and sperm pathologies that were just out of normal range. Group 3 included 6 infected patients with progressive motility r22%, low levels of LPO with a higher percentage of apoptosis and necrosis. Group 4 had 7 patients showing normal semen parameters, without GI, LPO ranged from 0% to 1% and a normal incidence of sperm pathologies. In all groups, LPO was inversely correlated with sperm motility and directly correlated with low semen quality. However, the LPO percentage was low in Group 3, in which sperm necrosis, concomitant with GI, was the predominant pathology. C11-BODIPY 581/591 is a useful labeling method for quantifying and localizing LPO in spermatozoa from patients with GI. The detection of LPO could be questionable in cases of sperm necrosis because in this pathology the plasma membrane is often disrupted and the lipidic probe is unable to intercalate within the phospholipidic bilayer.

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Section: Introductionmentioning

confidence: 99%

Lipid Peroxidation in Human Spermatozoa from Men with Genitourinary Infections

Cosci

Moretti

Collodel

2008

Systems Biology in Reproductive Medicine

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“…The prevalence of bacteriospermia in semen samples varies across different studies -51% (5) in Cottell et al's study, 35.7% (4) and 51.1% (6) in Lackner et al's studies, 56.9% (7) in Gdoura et al's study, and 63.9% (11) in Rodin et al's study. The prevalence of bacteriospermia in the present study was 18.6%, which is much lower than those previously reported.…”

Section: This Methods Proved Effective As None Of Their Cultures Hadmentioning

confidence: 92%

“…Also, reactive oxygen species, proteases and cytokines resulting from an increased PMN count have been considered the mechanism of sperm damage in a study by Gdoura et al (7) Using a COX-2 inhibitor, Lackner et al were able to reduce leucocytospermia and increase sperm count in patients with abacterial leucocytospermia. (4) In sexual reproduction, the female genital tract has lysozymes, phagocytes and lactic acid, which function as a natural defence mechanism against microbial infection from semen. However, since IVF procedures bypass the defence mechanism present in sexual reproduction (i.e.…”

Section: Discussionmentioning

confidence: 99%

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Is semen polymorphonuclear leucocytes count a good predictor of male genital tract infection?

Chen

Yu²,

Rajesh³

2013

smedj

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(1) Leucocytospermia often indicates an inflammatory condition, and is associated with possible infection and poor sperm activity. Bezold et al (2) have concluded that bacteriospermia, especially in sexually transmitted diseases, is a likely cause of male infertility and may jeopardise the pregnancy rate for in vitro fertilisation (IVF).Therefore, the detection of bacteriospermia is of high importance.Cumming and Carrell have concluded that it is more costeffective and prudent to first perform reflexive culture for leucocytospermic patients before proceeding with IVF.However, Lackner et al has shown that an increased PMN has low sensitivity and specificity in predicting bacteria in semen.One study has also demonstrated a 50% false positive rate in culture due to contamination during semen collection.(5) Thus, although it is common practice to order semen culture when PMN is increased, leucocytospermia is a poor marker for bacteriospermia or impaired semen quality. Directly detecting bacteria in semen is not feasible since it requires a Gram stain, which is not commonly used in semen analysis, (1) and has a high false negative rate. Cross-sectional studies by Lackner et al (6) and Gdoura et al (7) have also compared the culture positivity of the semen of patients with and without leucocytospermia.Since there has been no published studies on the Singapore population so far, we aimed to determine whether an increased PMN count in semen is a good predictor of the presence of male genital tract infection, which is detected by semen culture. Is semen polymorphonuclear leucocytes count a good predictor of male genital tract infection? M E TH O DS

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“…Eigene Untersuchungen ergaben, dass weder ein unmittelbarer Zusammenhang zwischen der Leukozytenkonzentration im Ejakulat und der Ejakulatqualität besteht, noch dass sich durch Antibiotikagabe eine Veränderung der Leukozytenkonzentration erzielen lässt. Zwar konnte gezeigt werden, dass Cyclooxygenase (COX)-2-Hemmer möglicherweise einen therapeutischen Effekt bei Leukozytospermie haben, allerdings wurden diese Präparate inzwischen vom Markt genommen [15]. aszendierenden Infektionen weitgehend geschützt.…”

Section: Leukozytospermieunclassified

Infektionen in der Reproduktionsmedizin

Keck¹,

Tempfer

Clad³

2008

Gynäkologe

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ZusammenfassungInfektionen spielen im Zusammenhang mit der Reproduktionsmedizin in vielerlei Hinsicht eine bedeutsame Rolle. Sie können als Ursache für Infertilität/Sterilität beim Mann bzw. der Frau verantwortlich sein. Da reproduktionsmedizinische Maßnahmen zu den invasiven Behandlungsmaßnahmen gehören, bergen sie per se ein Risiko für die Übertragung/Manifestation von Infektionen. Falls es durch Anwendung reproduktionsmedizinischer Maßnahmen zu einer Schwangerschaft kommt, muss an potenzielle Infektionsrisiken für das aus dieser Behandlung hervorgehende Kind gedacht werden. In den Richtlinien zur Durchführung assistierter reproduktionsmedizinischer Maßnahmen wird vor Behandlungsbeginn der Ausschluss/Nachweis bestimmter Infektionskrankheiten gefordert und damit impliziert, dass infizierte Patient(inn)en von den Behandlungsmaßnahmen prinzipiell auszuschließen sind. Im vorliegenden Beitrag wird ausführlich auf diese Aspekte eingegangen, und es wird aufgezeigt, dass es sich bei vielen potenziellen Infektionsrisiken eher um theoretische als um klinisch relevante Probleme handelt. Dieser Beitrag soll bei differenzialdiagnostischen und -therapeutischen Fragen zu Infektionen in der Reproduktionsmedizin als Entscheidungshilfe dienen. Schlüsselwörter AbstractInfectious diseases may play an important role in human reproduction: They can cause male/female infertility/sterility. ART (assisted reproductive techniques) can facilitate/induce infectious diseases. Infectious diseases can complicate a pregnancy arising from ART. Official guidelines for ART postulate that before treatment certain infectious diseases such as hepatitis and/or HIV should be excluded in both partners. This article addresses the above-mentioned issues and highlights that some of the potential infectious risk factors are theoretical risks rather than real clinical problems.

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Correlation of leukocytospermia with clinical infection and the positive effect of antiinflammatory treatment on semen quality

Cited by 88 publications

References 31 publications

Lipid Peroxidation in Human Spermatozoa from Men with Genitourinary Infections

Lipid Peroxidation in Human Spermatozoa from Men with Genitourinary Infections

Is semen polymorphonuclear leucocytes count a good predictor of male genital tract infection?

Infektionen in der Reproduktionsmedizin

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