Correlation of rotational thromboelastometry (ROTEM) parameters with platelet count and their ability to predict thrombocytopenia in dogs

Turner, Jennifer; Kutter, Annette P N; Sigrist, Nadja

doi:10.1016/j.rvsc.2019.08.007

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…For the following variables, percent change from baseline was calculated: platelet count, AUC ARA-tem, AUC ADP-tem, MCF Ex-tem, MCF In-tem, MCF Fib-tem, MCF Ap-tem, d-dimers as follows: (Xactual − Xbaseline)/Xbaseline. To assess the platelet contribution to the ROTEM delta results, the difference between MCF Ex-tem and Fib-tem was calculated [19] and is presented as "platelet-tem".…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Effect of Induced Endotoxemia on ROTEM S® and Platelet Parameters in Beagle Dogs Anaesthetized with Sevoflurane

Kutter,

Joerger,

Riond

et al. 2023

Animals

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Endotoxemia is thought to induce severe changes in coagulation status. In this study, blood samples from six beagle dogs receiving 1 mg/kg E. coli lipopolysaccharide (LPS) intravenously were analyzed to describe the concurrent changes in platelet count, platelet function assessed with impedance thromboaggregometry, thromboelastometry and d-dimers during artificially induced endotoxemia and its therapy with fluids and vasopressors at five timepoints (baseline, after LPS and 30 mL/kg Ringer’s acetate, during noradrenaline ± dexmedetomidine infusion, after a second fluid bolus and a second time after vasopressors). Results were analyzed for changes over time with the Friedman test, and statistical significance was set at p < 0.05. We found decreased platelet count and function and changes in all platelet-associated rotational thromboelastometry (ROTEM) variables indicating hypocoagulability, as well as increases in d-dimers indicating fibrinolysis within one hour of intravenous administration of LPS, with partial recovery of values after treatment and over time. The fast changes in platelet count, platelet function and ROTEM variables reflect the large impact of endotoxemia on the coagulation system and support repeated evaluation during the progress of endotoxemic diseases. The partial recovery of the variables after initiation of fluid and vasopressor therapy may reflect the positive impact of the currently suggested therapeutic interventions during septic shock in dogs.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Effect of Induced Endotoxemia on ROTEM S® and Platelet Parameters in Beagle Dogs Anaesthetized with Sevoflurane

Kutter,

Joerger,

Riond

et al. 2023

Animals

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“…Возникающие изменения в системе гемостаза организма экспериментальных животных являются результатом сложного, многокомпонентного взаимодействия тромбоцитов, факторов свертывания и клеточных элементов [9][10][11][12]. По результату проведенного поиска в имеющихся и доступных базах данных установлено, что на сегодняшний день представлено небольшое количество публикаций, в которых содержится подробное описание проводимых экспериментальных исследований с целью выявления межвидовых различий (между животными и человеком) в значениях показателей гемостаза.…”

Section: Discussionunclassified

Evaluation of Hemostatic System after Abdominal Aortic Replacement in Animals

Попель,

Моисеенко,

Островский

et al. 2023

Хирургия. Восточная Европа

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Цель. Изучить изменения в системе гемостаза организма животных с помощью метода ротационной тромбоэластометрии при протезировании брюшной аорты синтетическим и биологическим сосудистыми протезами. Материалы и методы. Исследование проведено на самках беспородных белых свиней (n=12). Животные были распределены на 2 группы: 1-я группа – с имплантацией синтетического сосудистого протеза из дакрона, покрытого модифицированным желатином, 2-я группа – с имплантацией биологического сосудистого протеза, изготовленного из бычьего ксеноперикарда. В тестах EXTEM, INTEM анализировали следующие показатели: CT (clotting time), CFT (clot formation time), угол α и MCF (maximum clot firmness). В тесте FIBTEM оценивали значения А10 и MCF. Для оценки вклада тромбоцитов (PLT) в формирование сгустка определяли их количество в периферической крови и рассчитывали значение MCF PLT по формуле: MCF PLT = MCF EXTEM – MCF FIBTEM. Результаты. У животных, которым был имплантирован биологический сосудистый протез из ксеноперикарда, отмечены более высокие значения времени свертывания крови и времени формирования сгустка в тестах EXTEM (на 14-е сутки Δ CT = 17,5 с.; p=0,041) и INTEM (на 7-е сутки Δ CFT = 83,0 с.; p=0,005; на 14-е сутки Δ CT = 69,0 с.; p=0,004; на 21-е сутки Δ CT = 25,0 с.; p=0,037), по сравнению с животными, у которых применяли синтетический протез. По результатам корреляционного анализа выявлена статистически значимая связь в значениях MCF PLT и угла α в тестах EXTEM (ρ=0,276; р=0,032) и INTEM (ρ=0,274; р=0,034). Кроме того, установлена статистически значимая корреляция между значениями MCF PLT и значениями MCF EXTEM (ρ=0,290; р=0,025). На протяжении всего периода наблюдения при применении биологического сосудистого протеза тромботических осложнений не наблюдали, а в группе с синтетическим протезом у 2 животных при выведении из эксперимента обнаружили полный тромбоз протеза в сроке 3 месяца после операции. Заключение. Более высокие значения времени формирования сгустка в тестах EXTEM и INTEM при сохранении значений остальных параметров в пределах границ референтного интервала указывают на более медленное начало образования фибрина и, соответственно, на более низкую тромбогенность биологического сосудистого протеза, по сравнению с синтетическим сосудистым протезом. Purpose. To evaluate changes in the hemostatic system of the animal body during abdominal aortic replacement with synthetic and biological vascular grafts using rotational thromboelastometry. Materials and methods. The study was performed on female white pigs (n=12). The animals were divided into 2 groups: group 1 – implantation of a synthetic vascular graft made of Dacron coated with modified gelatin, group 2 – with implantation of a biological vascular graft made of bovine xenopericardium. In the EXTEM and INTEM tests, the following parameters were analyzed: CT (clotting time), CFT (clot formation time), α angle and MCF (maximum clot firmness). In the FIBTEM test, A10 and MCF values were evaluated. To evaluate the contribution of platelets to clot formation, the number of platelets in peripheral blood was measured and MCF PLT value was calculated using the formula: MCF PLT = MCF EXTEM – MCF FIBTEM. Results. The results of this study show that in animals implanted with biological vascular graft from bovine xenopericardium, the values of clotting time and clot formation time in EXTEM (on day 14, Δ CT = 17.5 s; p=0.041) and INTEM (on day 7, Δ CFT = 83.0 s; p=0.005; on day 14, Δ CT = 69.0 s; p=0.004; on day 21, Δ CT = 25.0 s; p=0.037) tests were higher than in animals with synthetic vascular graft. According to the results of correlation analysis, a statistically significant relationship was revealed in the values of MCF PLT and angle α in the EXTEM (p=0.276; p=0.032) and INTEM (p=0.274; p=0.034) tests. In addition, a statistically significant correlation was established between the values of MCF PLT and the values of MCF EXTEM (p=0.290; p=0.025). There were no thrombotic complications in the group of animals with biological vascular prosthesis, but in the group with synthetic vascular prosthesis, graft thrombosis was observed in two animals removed from the experiment at 3 months after surgery. Сonclusion. No thrombotic complications were observed during the whole period of experiment in pigs with implanted biological vascular graft.

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Reference intervals for rotational thromboelastometry measurements using the ROTEM® delta device in dogs

Pereira

Rohn

Mischke

2020

Research in Veterinary Science

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Correlation of rotational thromboelastometry (ROTEM) parameters with platelet count and their ability to predict thrombocytopenia in dogs

Cited by 4 publications

References 39 publications

Evaluation of the Effect of Induced Endotoxemia on ROTEM S® and Platelet Parameters in Beagle Dogs Anaesthetized with Sevoflurane

Evaluation of the Effect of Induced Endotoxemia on ROTEM S® and Platelet Parameters in Beagle Dogs Anaesthetized with Sevoflurane

Evaluation of Hemostatic System after Abdominal Aortic Replacement in Animals

Reference intervals for rotational thromboelastometry measurements using the ROTEM® delta device in dogs

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