Correlation of serology with morphological changes in gastric biopsy of H. pylori infection

Rajan, Amit; Ganguli, Prosenjit; Pathak, Niloy; Ranjan, Amar; Thapa, Ragini; Tripathi, Sarsij

doi:10.18203/2320-6012.ijrms20171599

Cited by 5 publications

(11 citation statements)

References 7 publications

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“…However, the total agreement between the H & E test and stool antigen test was 93.6%, so the difference between the two tests results was significant. This was comparable to the results reported by [16]the prevalence of positive tests for H. pylori was higher in Giemsa stain 55% as compared to H&E test 47%.…”

Section: Discussionsupporting

confidence: 90%

“…The sensitivity and specificity of serum antibody test were 98.4% and 82.6% respectively. These results were similar to those of Rajan, [16] who found lower values for specificity 59.25% and 90% For sensitivity. In another study done by [17] there results were the diagnostic odds ratios for serology, and stool antigen test, the sensitivity was 95% estimated at a fixed specificity (84%) for serology, which is in a good accordance with our data.…”

Section: Discussionsupporting

confidence: 90%

“…In the histopathological unit, the Vol. 16 biopsy specimens of all 171 patients were fixed in 10% buffered formalin for at least 24hrs, and then embaded in paraffin wax. Hematoxylin and Eosin (H and E) staining and modified Giemsa staining were performed on tissue sections of each case.…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of Invasive and Non-Invasive Methods for the Diagnosis of H. pylori in Dyspepsia Patients

Mawlood¹,

Kawther²,

Balaky³

2019

DJM

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Background: H. pylori has been established as the major causative agent of chronic gastritis, and represents the main etiological cause of other gastroduodenal diseases such as peptic ulceration, gastric lymphoma, and gastric cancer. Therefore, screening and treatment of the bacterium is an important strategy for preventing gastric cancer. Objective: To compare some invasive classical histological tests to non-invasive serological antibody and stool antigen tests for the diagnosis of H. pylori infections. Patients and Methods:The study population comprised of 171 patients with symptoms of dyspepsia and other gastritis related symptoms. Three biopsy specimens were taken and collected from the stomach and sent for histopathologic study. H and E staining and modified Giemsa staining were performed on tissue sections of each case. Serology antibody and Stool antigen tests were also used as nan-invasive tests in this study. Results: Four tests had been done to detect the presence of H. pylori Bacteria. The mean age (+ SD) of the studied sample was 43.8 + 14.7 years, ranging from 20 to 74 years. The median was 44 years. The total agreement between the serum Ab test and the stool Ag test was 88.3%. Significant differences were detected between the two tests (p < 0.001). It is evident that the serum Ab test is highly sensitive (sensitivity = 98.4%), but the Predictive value positive (76.3%) was not so high. The Giemsa test was highly specific (specificity = 98.2%), and highly sensitive (sensitivity = 93.5%). Conclusion: The main advantage of histopathology tests is not only restricted to detection purpose, it also can detect any abnormalities of gastric mucosa by bacteria such as peptic ulcer bleeding, atrophic gastritis, intestinal metaplasia, and gastric cancer. The modified Giemsa staining method could be used as a method of choice for the detection of H. pylori due to its sensitivity, simplicity and consistency.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 90%

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Evaluation of Invasive and Non-Invasive Methods for the Diagnosis of H. pylori in Dyspepsia Patients

Mawlood¹,

Kawther²,

Balaky³

2019

DJM

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“…The discovery of Helicobacter pylori (H. pylori) in 1982 was the starting point of a revolution concerning the concepts and management of gastroduodenal diseases. 1,2 H. pylori represents one of the most common and medically important infections worldwide. 3 Chronic infection due to this pathogen is now believed to account for the majority of cases of chronic gastritis.…”

Section: Introductionmentioning

confidence: 99%

“…4 In addition, H. pylori is the major cause of gastric carcinogenesis and other gastric diseases, such as duodenal and gastric non-ulcer dyspepsia, gastroduodenal ulcers, and gastric mucosa-associated lymphoid tissue lymphoma. 1,5 In recent years, a variety of extra digestive disorders, including cardiovascular diseases, liver diseases and autoimmune disorders have also been associated with infections caused by H. pylori. 6 Gastric cancer is the third most common cause of death caused and related to cancer worldwide.…”

Section: Introductionmentioning

confidence: 99%

Incidence of Helicobacter Pylori Infections in a group of patients in Erbil City, Kurdistan Region, Iraq and Its Association with Gastritis and Adenocarcinoma

Salim¹,

Balaky²,

Al-Rawi³

et al. 2020

Preprint

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Background: Adenocarcinoma is one of the most common causes of Gastric cancer related deaths worldwide. Helicobacter pylori is the causative agent of most cases of gastritis, it can cause chronic active gastritis and known as a risk factor for the development of gastric cancer. This study aimed to assess the prevalence of H. pylori among patients with symptoms of dyspepsia and other gastritis related symptoms and its association with adenocarcinoma.Methods: This study was carried out during the period of January 2018 to October 2019 with a total of 227 patients with gastritis related symptoms. The presence of H. pylori was detected by Rapid Urease Test (RUT) and histo-pathological tests using biopsy specimens. Statistical Analysis was done by using Chi-square test. P < 0.05 was considered to be statistically significant.Results: From the total of 227 patients with gastritis related symptoms, 26 cases (13.61%) were diagnosed with adenocarcinoma. Their ages were between 13 and 90 years with mean of 47.81± 18.23. The result showed that low severity prevalence of H. pylori was highest (111 cases) compared to 17 and 63 cases for high and moderate severity, respectively. Comparison between positive low, moderate, and high H. pylori cases for rapid urease test was highly significant (P<0.000). The results showed no association between H. pylori severity across various age groups and gender. Moreover, goodness of fit test for metaplasia, activity, glandular atrophy, and endoscopic finding across severity status of H. pylori showed highly significant. Four composite categorized groups were initiated based on positive/negative prevalance of H. pylori and adenocarcinoma status. Results revealed statistical significance between combination of H. pylori and adenocarcinoma with inflammation, lymphoid aggregate, metaplasia, activity of neutrophils, glandular atrophy, rapid urease test, and endoscopic findings.Conclusion: Histopathology tests are reliable diagnostic tools for the detection of H. pylori. Data showed that H. pylori was seen more in middle age patients with mucosal lymphoid follicle formation and more than one third of patients with adenocarcinoma. Therefore, screening of these infections is an important strategy for preventing gastric adenocarcinoma.

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Comparison of invasive histological and molecular methods in the diagnosis of Helicobacter pylori from gastric biopsies of Sudanese patients: a cross-sectional study

et al. 2022

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Background: The continuous rise in the number of patients suffering from Helicobacter pylori is probably due to the changes in modern life. Nowadays, patients suffering from gastrointestinal problems are diagnosed through invasive and non-invasive techniques. The choice of a diagnostic test is influenced by factors such as the tests' sensitivity and specificity, the clinical conditions, and the cost-effectiveness of the testing strategy. This study aimed to compare molecular detection methods of H. pylori by polymerase chain reaction (PCR) targeting the 16S rRNA, ureA and glmM genes with an invasive histopathological technique. Methods: 290 gastric biopsies were collected using gastrointestinal endoscopy from patients with gastritis symptoms in different hospitals in Khartoum state. Two gastric biopsies were collected from each patient for PCR and histopathology. Results: A total of 103 (35.5%) samples were positive by histopathological examination, 88 (30.3%) by 16S rRNA, 39 (13.4%) by glmM gene, and 56 (19.3%) by ureA gene. The highest sensitivity was observed in 16S rRNA (46.6%), followed by glmM (24.3%) and ureA (23.3%). While the best specificity was observed in glmM gene (92.5%), followed by ureA (82.3%) and 16S rRNA (78.6%). Conclusion: PCR test targeting the 16S rRNA gene exhibited the best results for molecular detection of H. pylori compared to other genes.

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Correlation of serology with morphological changes in gastric biopsy of H. pylori infection

Cited by 5 publications

References 7 publications

Evaluation of Invasive and Non-Invasive Methods for the Diagnosis of H. pylori in Dyspepsia Patients

Evaluation of Invasive and Non-Invasive Methods for the Diagnosis of H. pylori in Dyspepsia Patients

Incidence of Helicobacter Pylori Infections in a group of patients in Erbil City, Kurdistan Region, Iraq and Its Association with Gastritis and Adenocarcinoma

Comparison of invasive histological and molecular methods in the diagnosis of Helicobacter pylori from gastric biopsies of Sudanese patients: a cross-sectional study

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