Correlation of Total Antioxidant Capacity with Reactive Oxygen Species (ROS) Consumption Measured by Oxidative Conversion

Çekiç, Sema Demirci; Çetinkaya, Aydan; Avan, Aslı Neslihan; Apak, Reşat

doi:10.1021/jf3051297

Cited by 39 publications

(12 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Synthetic antioxidants, such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) are often used as food additives. However, they have been associated with liver damage and carcinogenesis [ 4 , 5 , 7 ]. Natural antioxidants are an interesting alternative in view of their variety of structures and chemical interactions, as well as the numerous biological activities they can perform.…”

Section: Introductionmentioning

confidence: 99%

Study of Polyphenol Content and Antioxidant Capacity of Myrianthus Arboreus (Cecropiaceae) Root Bark Extracts

2015

View full text Add to dashboard Cite

In order to evaluate the therapeutic potential of polyphenolic extracts from root bark of M. arboreus, we have determined the content of various polyphenols in aqueous and ethanol (EtOH) extract as well as two sub-fractions of the latter: ethyl acetate (EAc) and hexane (Hex). The total phenols, flavonoids, hydroxycinnamic acids and proanthocyanidins have been determined for all studied extracts/fractions by spectrophotometric methods. Both TP content (331.5 ± 2.5 mg GAE/g) and HCA content (201 ± 1.5 mg CAE/g) were determined to be the highest in EAc fraction of EtOH extract. All studied extracts were however determined to have a low content in flavonoids. The determination of antioxidant capacities of the studied extracts has also been performed by the following in vitro antioxidant tests: DPPH scavenging, phosphomolybdenum method and oxygen radical absorbance (ORACFl and ORACPRG) assay. The results of the DPPH free radical and ORACFl assays showed that there is no significant difference between the EAc fraction and Oligopin®, but the EAc fraction exhibited the highest antioxidant capacity as determined by the phosphomolybdenium method. In addition, the EtOH extract was determined to have the same antioxidant efficiency as the synthetic antioxidant BHT or commercial extract Oligopin® by phosphomolybdenum method. On the other hand, a positive correlation (r < 0.6) was found between different classes of polyphenols and the results of the phosphomolybdenum method, ORACFl as well as ORACPRG, except for the DPPH assay, for which a negative correlation was indicated (r < 0.62). Interestingly, it seems that the content in hydroxycinnamic acids played a big role in all assays with r < 0.9. According to the present study, EAc fraction and EtOH extract should be further studied for the potential use in the pharmaceutical and food industry.

show abstract

Section: Introductionmentioning

confidence: 99%

Study of Polyphenol Content and Antioxidant Capacity of Myrianthus Arboreus (Cecropiaceae) Root Bark Extracts

2015

View full text Add to dashboard Cite

show abstract

“…Normally, the difference in oxidative status of a medium undergoing ROS/RNS attack, to which a food or biological extract was added, should correlate to TAC of the extract under investigation. 13 Additionally, antioxidant activity needs to be correlated to electrochemical behavior of antioxidants. As required parameters, a standardized TAC method (i) utilizes a suitable oxidant that actually is or is a simulator of a biologically relevant reactive species; (ii) is simple, practical, and versatile; (iii) uses a method with a defined end-point and a clear chemical mechanism; (iv) has readily available and preferably low-cost instrumentation; (v) is reproducible with good within-run and between-run precision; (vi) can assay both hydrophilic and lipophilic antioxidants; (vii) does not generate new reactive species that may cause under-or overestimated TAC readings; and (viii) is suitable for "high-throughput" analysis for routine quality control of food and biological extracts.…”

mentioning

confidence: 99%

Antioxidant Activity/Capacity Measurement. 1. Classification, Physicochemical Principles, Mechanisms, and Electron Transfer (ET)-Based Assays

Apak

Özyürek

Güçlü

et al. 2016

J. Agric. Food Chem.

Self Cite

609

379

View full text Add to dashboard Cite

Because there is no widely adopted "total antioxidant parameter" as a nutritional index for labeling food and biological fluids, it is desirable to establish and standardize methods that can measure the total antioxidant capacity (TAC) level directly from plant-based food extracts and biological fluids. In this review, we (i) present and classify the widely used analytical approaches (e.g., in vitro and in vivo, enzymatic and nonenzymatic, electron transfer (ET)- and hydrogen atom transfer (HAT)-based, direct and indirect assays) for evaluating antioxidant capacity/activity; (ii) discuss total antioxidant capacity/activity assays in terms of chemical kinetics and thermodynamics, reaction mechanisms, and analytical performance characteristics, together with advantages and drawbacks; and (iii) critically evaluate ET-based methods for analytical, food chemical, biomedical/clinical, and environmental scientific communities so that they can effectively use these assays in the correct places to meet their needs.

show abstract

“…Intracellular ROS levels are directly proportional to the antioxidant capacity of cells and the extent of oxidative stress injury to the cells (Çekiç, Çetinkaya, Avan, & Apak, 2013 F I G U R E 6 Effect of three hazelnut peptides (EW, ADGF, and DWDPK) at different concentrations (50, 100, and 500 μg/ml) on the protein expression levels of NOX4 and p22phox in Ang II-induced HUVECs. HUVECs were treated with or without different concentrations of three hazelnut peptides (50, 100, and 500 μg/ml) and Vc, and then induced with Ang II (10 −7 mol/L) for 6 hr.…”

Section: Discussionmentioning

confidence: 99%

Protective role of hazelnut peptides on oxidative stress injury in human umbilical vein endothelial cells

Ren

Wang

et al. 2018

J Food Biochem

View full text Add to dashboard Cite

The crude protein hydrolysates of wild hazel have good immunoregulation and antioxidation effects. However, the components responsible for their antioxidation effect remain unknown. In this study, six antioxidative peptides (EW, DWDPK, ADGF, SGAF, ETTL, and AGGF) were tested for their protective effects on oxidative stress injury in human umbilical vein endothelial cells (HUVECs). The results demonstrated that the six peptides are nontoxic and have a protective effect on oxidative stress injury induced by Ang II. Three peptides (EW, ADGF, and DWDPK) inhibited the morphological changes, downregulated the content of lactate dehydrogenase and malondialdehyde, upregulated the activity of antioxidant enzymes catalase, total superoxide dismutase and glutathione peroxidase, and scavenged reactive oxygen species (ROS) in HUVECs. Quantitative reverse transcriptive polymerase chain reaction and western blot assays indicated that these three peptides regulated NADPH oxidase activity and ROS production by reducing NOX4 and p22phox levels. Overall, they have a significant protective effect against oxidative stress injury and have potential application in developing new functional foods. Practical applications Corylus heterophylla Fisch is a good quality wild hazel distributed in Northeast China. Wild hazelnut of the species C. heterophylla Fisch was selected as experimental object and has high nutritive values and have abundant proteins (20%–30%), fats (40%–50%), carbohydrates (13%–24%), dietary fibers (8.2%–9.6%), vitamins, and micronutrients. Our results indicate that hazelnut peptides (EW, ADGF, and DWDPK) can ensure normal growth of cells by protecting important antioxidant enzyme systems, by enhancing antioxidant defense, by directly affecting nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity, and by reducing intracellular reactive oxygen species (ROS) production in HUVECs, indicating that the three antioxidative peptides have a protective effect against Ang II‐induced oxidative stress injury. Therefore, the antioxidative peptides from C. heterophylla Fisch may be a promising candidate for functional food ingredients and/or pharmaceuticals.

show abstract

Correlation of Total Antioxidant Capacity with Reactive Oxygen Species (ROS) Consumption Measured by Oxidative Conversion

Cited by 39 publications

References 45 publications

Study of Polyphenol Content and Antioxidant Capacity of Myrianthus Arboreus (Cecropiaceae) Root Bark Extracts

Study of Polyphenol Content and Antioxidant Capacity of Myrianthus Arboreus (Cecropiaceae) Root Bark Extracts

Antioxidant Activity/Capacity Measurement. 1. Classification, Physicochemical Principles, Mechanisms, and Electron Transfer (ET)-Based Assays

Protective role of hazelnut peptides on oxidative stress injury in human umbilical vein endothelial cells

Contact Info

Product

Resources

About