Effects of thermal aging on tensile and Charpy impact properties in 16MND5 steel was investigated, which were aged at 500°C for 0 h, 1000 h, 3000 h, 5000 h. A significant decrease in the yield stress and ultimate tensile strength was observed after thermal aging, while the elongation exhibited a slight decrease follow by an increase aged for 5000 h. What's more, the ductile-to-brittle transition temperature (DBTT) showed a remarkable increase with the prolongation of thermal aging duration. These facts indicate thermal aging caused embrittlement of the steel, which was further investigated by microstructure observation of SEM. The results show cleavage fracture after thermal aging. Furthermore, experimental results at 350°C thermal aging temperature originated from the previous literature were used to analysis the effect of thermal aging temperature. Thus, thermal embrittlement should be taken seriously.