Corrosion resistance and cytotoxicity studies of DLC, TiN and TiCN films coated on 316L stainless steel

Sunthornpan, Narin; Watanabe, Shuichi; Moolsradoo, Nutthanun

doi:10.1088/1742-6596/1144/1/012013

Cited by 4 publications

(4 citation statements)

References 17 publications

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“…Both films exhibited strong hydrophilicity. Similar results were reported by Khan et al for TiN [23] and Sunthornpan et al for TiCN [24]. When AISI 316 L was coated with a Ti-DLC film, the water contact angle increased to 96.1° ± 0.4° and showed significant hydrophobicity, which is mainly due to the chemical properties of DLC.…”

Section: Surface Characteristicssupporting

confidence: 88%

Evaluation of Biocompatibility of 316 L Stainless Steels Coated with TiN, TiCN, and Ti-DLC Films

et al. 2022

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In this study, TiN, TiCN, and Ti-diamond-like carbon (Ti-DLC) films were coated on 316 L stainless steel (AISI 316 L) substrate surface by physical vapor deposition. The biocompatibility of the three films (TiN, TiCN, and Ti-DLC) and three metals (AISI 316 L, Ti, and Cu) was compared on the basis of the differences in the surface morphology, water contact angle measurements, CCK-8 experiment results, and flow cytometry test findings. The biocompatibility of the TiN and TiCN films is similar to that of AISI 316 L, which has good biocompatibility. However, the biocompatibility of the Ti-DLC films is relatively poor, which is mainly due to the inferior hydrophobicity and large amount of sp2 phases. The presence of TiC nanoclusters on the surface of the Ti-DLC film aggravates the inferior biocompatibility. Compared to the positive Cu control group, the Ti-DLC film had a higher cell proliferation rate and lower cell apoptosis rate. Although the Ti-DLC film inhibited cell survival to a certain extent, it did not show obvious cytotoxicity. TiN and TiCN displayed excellent performance in promoting cell proliferation and reducing cytotoxicity; thus, TiN and TiCN can be considered good orthodontic materials, whereas Ti-DLC films require further improvement.

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Section: Surface Characteristicssupporting

confidence: 88%

Evaluation of Biocompatibility of 316 L Stainless Steels Coated with TiN, TiCN, and Ti-DLC Films

et al. 2022

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“…Materials are considered biocompatible for viability percentages over 70%, according to the criteria of the ISO10993-5 standard. 63 To perform this assay, a multicolor confocal microscope ( Zeiss, LSM780 ) is used. This imaging is not performed on IP-S samples since the glass substrate is too thick to image with the confocal microscope.…”

Section: Methodsmentioning

confidence: 99%

“…The resulting solution contains approximately 2 μM of calcein-AM and 4 μM of EthD-1. Materials are considered biocompatible for viability percentages over 70%, according to the criteria of the ISO10993-5 standard . To perform this assay, a multicolor confocal microscope ( Zeiss, LSM780 ) is used.…”

Section: Methodsmentioning

confidence: 99%

Cellular Interaction of Bone Marrow Mesenchymal Stem Cells with Polymer and Hydrogel 3D Microscaffold Templates

Costa

Adão

Maibohm

et al. 2022

ACS Appl. Mater. Interfaces

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Biomimicking biological niches of healthy tissues or tumors can be achieved by means of artificial microenvironments, where structural and mechanical properties are crucial parameters to promote tissue formation and recreate natural conditions. In this work, three-dimensional (3D) scaffolds based on woodpile structures were fabricated by two-photon polymerization (2PP) of different photosensitive polymers (IP-S and SZ2080) and hydrogels (PEGDA 700) using two different 2PP setups, a commercial one and a customized one. The structures’ properties were tuned to study the effect of scaffold dimensions (gap size) and their mechanical properties on the adhesion and proliferation of bone marrow mesenchymal stem cells (BM-MSCs), which can serve as a model for leukemic diseases, among other hematological applications. The woodpile structures feature gap sizes of 25, 50, and 100 μm and a fixed beam diameter of 25 μm, to systematically study the optimal cell colonization that promotes healthy cell growth and potential tissue formation. The characterization of the scaffolds involved scanning electron microscopy and mechanical nanoindenting, while their suitability for supporting cell growth was evaluated with live/dead cell assays and multistaining 3D confocal imaging. In the mechanical assays of the hydrogel material, we observed two different stiffness ranges depending on the indentation depth. Larger gap woodpile structures coated with fibronectin were identified as the most promising scaffolds for 3D BM-MSC cellular models, showing higher proliferation rates. The results indicate that both the design and the employed materials are suitable for further assays, where retaining the BM-MSC stemness and original features is crucial, including studies focused on BM disorders such as leukemia and others. Moreover, the combination of 3D scaffold geometry and materials holds great potential for the investigation of cellular behaviors in a co-culture setting, for example, mesenchymal and hematopoietic stem cells, to be further applied in medical research and pharmacological studies.

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“…However, DLC film has the problem of poor bonding strength with the substrate. Researchers have improved the bonding strength between DLC film and the substrate by using multilayer films method [10,11]. Sheng Liyuan et al have deposited Ti/TiN multilayer films by high-power DC magnetron sputtering [7], analyzed the influence of different layers on hardness, and found that selecting appropriate layers could improve the hardness.…”

Section: Introductionmentioning

confidence: 99%

Friction and Wear Properties of Multilayer Films Designed on Tantalum Substrate

Guo

Zheng

Huang

et al. 2022

MSF

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In this paper, a titanium nitride film (TiN) monolayer film was deposited on a pure Tantalum (Ta) substrate with Ti as an intermediate layer by magnetron sputtering technique, and a Ta/Ti/DLC (diamond-like carbon)/Ti/TiN/DLC multilayer film was designed. Raman spectroscopy and scanning electron microscopy were used to observe the film structure and morphology. Friction and wear properties of Ta/Ti/TiN monolayer film and Ta/Ti/DLC/Ti/TiN/DLC multilayer film were analyzed by UMT-3 tester. The results show that the surface of multilayer film is denser and better bonded than TiN monolayer film. Under dry friction conditions, the friction coefficient of TiN monolayer film is stable at about 0.45. In contrast, the friction coefficient of DLC multilayer film remains around 0.15 with very small fluctuation during the whole friction process. This is determined by the inherent characteristics of DLC film, which is composed of sp2-C graphite structure and sp3-C diamond structure. From the analysis of the wear mechanism, the Ta substrate mainly undergoes adhesive wear and abrasive wear, however, the TiN monolayer film and Ta/Ti/DLC/Ti/TiN/DLC multilayer films mainly undergo abrasive wear mechanism. The friction coefficient of TiN monolayer fluctuates more than that of dry friction conditions in the body-liquid environment, and finally stabilize at about 0.5. The friction coefficient of DLC multilayer films does not differ much from those of dry friction conditions. This is because the TiN monolayer film generates a large amount of abrasive chips to fill the scratches in the body fluid environment, accompanied by the peeling and flaking of the compacted abrasive chips, thus the friction coefficient fluctuates greatly. The DLC multilayer films also undergoes graphitization transfer. In addition, the free hanging bonds of DLC film are passivated, which reduces the degree of adhesive wear. In summary, the DLC multilayer film has better biocompatibility, wear resistance, and stronger bonding.

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Corrosion resistance and cytotoxicity studies of DLC, TiN and TiCN films coated on 316L stainless steel

Cited by 4 publications

References 17 publications

Evaluation of Biocompatibility of 316 L Stainless Steels Coated with TiN, TiCN, and Ti-DLC Films

Evaluation of Biocompatibility of 316 L Stainless Steels Coated with TiN, TiCN, and Ti-DLC Films

Cellular Interaction of Bone Marrow Mesenchymal Stem Cells with Polymer and Hydrogel 3D Microscaffold Templates

Friction and Wear Properties of Multilayer Films Designed on Tantalum Substrate

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