Corticosterone increases FGF-2 (bFGF) immunoreactivity in the substantia nigra of the rat

Chadi, Gerson; Rosén, L.; Cintra, A.; Tinner, B.; Zoli, Michèle; Pettersson, Ralf F.; Fuxé, Kjell

doi:10.1097/00001756-199306000-00047

Cited by 51 publications

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“…Animals were deeply anaesthetized with sodium pentobarbital and sacrifi ced by a transcardiac perfusion with 100 ml isotonic saline at room temperature followed by 500 ml of fi xation fl uid (4ºC) over a period of 6 minutes 4,21,22 . The fi xative consisted of 4% paraformaldehyde (w/v, Merck, Germany) in 0.1 M phosphate buffer, pH 6.9.…”

Section: Tissue Preparation and Stainingmentioning

confidence: 99%

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Differential astroglial responses in the spinal cord of rats submitted to a sciatic nerve double crush treated with local injection of cultured Schwann cell suspension or lesioned spinal cord extract: implications on cell therapy for nerve repair

Dallo¹,

Reichert²,

Júnior³

et al. 2007

Acta Cir. Bras.

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Differential astroglial responses in the spinal cord of rats -ORIGINAL ARTICLE NeuroregenerationDifferential astroglial responses in the spinal cord of rats submitted to a sciatic nerve double crush treated with local injection of cultured Schwann cell suspension or lesioned spinal cord extract. Implications on cell therapy for nerve repair 1 Respostas astrocitárias na medula espinal do rato submetido ao esmagamento duplo do nervo ciático e tratado com injeção local de suspensão de células de Schwann cultivadas ou de extrato de medula espinal lesada. Implicações na terapia celular para o reparo do nervo ABSTRACT Purpose: Reactive astrocytes are implicated in several mechanisms after central or peripheral nervous system lesion, including neuroprotection, neuronal sprouting, neurotransmission and neuropathic pain. Schwann cells (SC), a peripheral glia, also react after nerve lesion favoring wound/repair, fi ber outgrowth and neuronal regeneration. We investigated herein whether cell therapy for repair of lesioned sciatic nerve may change the pattern of astroglial activation in the spinal cord ventral or dorsal horn of the rat. Methods: Injections of a cultured SC suspension or a lesioned spinal cord homogenized extract were made in a reservoir promoted by a contiguous double crush of the rat sciatic nerve. Local injection of phosphate buffered saline (PBS) served as control. One week later, rats were euthanized and spinal cord astrocytes were labeled by immunohistochemistry and quantifi ed by means of quantitative image analysis. Results: In the ipsilateral ventral horn, slight astroglial activations were seen after PBS or SC injections, however, a substantial activation was achieved after cord extract injection in the sciatic nerve reservoir. Moreover, SC suspension and cord extract injections were able to promote astroglial reaction in the spinal cord dorsal horn bilaterally. Conclusion: Spinal cord astrocytes react according to repair processes of axotomized nerve, which may infl uence the functional outcome. The event should be considered during the neurosurgery strategies. Key words: Sciatic nerve lesion. Astrocyte. Neuronal protection. Neuroplasticity. Neuroregeneration. Pain. Review. RESUMOObjetivo: Astrócitos reativos participam de vários mecanismos após lesões do sistema nervoso central e periférico, os quais incluem neuroproteção, brotamento neuronal, neurotransmissão e dor neuropática. As células de Schwann (CS), um tipo de glia periférica, também reagem com a lesão do nervo, podendo interferir com o reparo e cicatrização, crescimento de fi bras e regeneração neuronais. Investigamos aqui a possibilidade da terapia celular para o reparo do nervo ciático poder alterar o padrão da ativação astrocitária nos cornos anterior e posterior da medula espinal do rato. Métodos: Suspensão de CS cultivadas ou extrato homogeneizado de medula espinal lesada de rato foram inoculados num reservatório feito a partir de dois esmagamentos aplicados no nervo ciático do rato distantes 0,5mm entre si. Injeção local de salina t...

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Section: Tissue Preparation and Stainingmentioning

confidence: 99%

“…Fields of measurements were sampled in the anterior and posterior horns (gray matter), bilaterally. The procedures using a Kontron-Zeiss KS400 image analyzer (Germany) have been described previously 4,21,26,28 . Briefl y, a television camera acquired images from the microscope (63x objective).…”

Section: Semiquantitative Microdensitometric Image Analysismentioning

confidence: 99%

Differential astroglial responses in the spinal cord of rats submitted to a sciatic nerve double crush treated with local injection of cultured Schwann cell suspension or lesioned spinal cord extract: implications on cell therapy for nerve repair

Dallo¹,

Reichert²,

Júnior³

et al. 2007

Acta Cir. Bras.

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“…bFGF immunohistochemistry employing a bFGF polyclonal antibody shows bFGF immunoreactivity in the cytoplasm of neurons and in the nuclei of glial cells (42)(43)(44)(56)(57)(58)(59) widely distributed in the developing and mature rodent central nervous system (60). However, the use of monoclonal bFGF antiserum has revealed the protein in the nuclei of glial cells throughout the forebrain and the midbrain as well as in the nuclei of some neuronal cells like those of layer II of retrosplenial granular cortex, the CA2 region of the hippocampus, the indusium griseum and the fasciola cinerea (45,61).…”

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confidence: 99%

Analysis of trophic responses in lesioned brain: focus on basic fibroblast growth factor mechanisms

Chadi

Fuxé

1998

Braz J Med Biol Res

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The actions of fibroblast growth factors (FGFs), particularly the basic form (bFGF), have been described in a large number of cells and include mitogenicity, angiogenicity and wound repair. The present review discusses the presence of the bFGF protein and messenger RNA as well as the presence of the FGF receptor messenger RNA in the rodent brain by means of semiquantitative radioactive in situ hybridization in combination with immunohistochemistry. Chemical and mechanical injuries to the brain trigger a reduction in neurotransmitter synthesis and neuronal death which are accompanied by astroglial reaction. The altered synthesis of bFGF following brain lesions or stimulation was analyzed. Lesions of the central nervous system trigger bFGF gene expression by neurons and/or activated astrocytes, depending on the type of lesion and time post-manipulation. The changes in bFGF messenger RNA are frequently accompanied by a subsequent increase of bFGF immunoreactivity in astrocytes in the lesioned pathway. The reactive astrocytes and injured neurons synthesize increased amount of bFGF, which may act as a paracrine/autocrine factor, protecting neurons from death and also stimulating neuronal plasticity and tissue repair.

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“…The actions of glial neurotrophic factors such as FGF-2 and S100ß have been described to central neurons and also investigated recently in the peripheral nervous system [8][9][10] . Despite the presence of S100ß in the satellite glial cells of the DRG and in the Schwann cells of the sciatic nerves, the cellular change the S100ß in those cells of axotomized sensory ganglia is still a material of a detailed investigation.…”

Section: Introductionmentioning

confidence: 99%

S100ß and fibroblast growth factor-2 are present in cultured Schwann cells and may exert paracrine actions on the peripheral nerve injury

et al. 2008

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PURPOSE: The neurotrophic factor fibroblast growth factor-2 (FGF-2, bFGF) and Ca++ binding protein S100ß are expressed by the Schwann cells of the peripheral nerves and by the satellite cells of the dorsal root ganglia (DRG). Recent studies have pointed out the importance of the molecules in the paracrine mechanisms related to neuronal maintenance and plasticity of lesioned motor and sensory peripheral neurons. Moreover, cultured Schwann cells have been employed experimentally in the treatment of central nervous system lesions, in special the spinal cord injury, a procedure that triggers an enhanced sensorymotor function. Those cells have been proposed to repair long gap nerve injury. METHODS: Here we used double labeling immunohistochemistry and Western blot to better characterize in vitro and in vivo the presence of the proteins in the Schwann cells and in the satellite cells of the DRG as well as their regulation in those cells after a crush of the rat sciatic nerve. RESULTS: FGF-2 and S100ß are present in the Schwann cells of the sciatic nerve and in the satellite cells of the DRG. S100ß positive satellite cells showed increased size of the axotomized DRG and possessed elevated amount of FGF-2 immunoreactivity. Reactive satellite cells with increased FGF-2 labeling formed a ring-like structure surrounding DRG neuronal cell bodies.Reactive S100ß positive Schwann cells of proximal stump of axotomized sciatic nerve also expressed higher amounts of FGF-2. CONCLUSION: Reactive peripheral glial cells synthesizing FGF-2 and S100ß may be important in wound repair and restorative events in the lesioned peripheral nerves.

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Corticosterone increases FGF-2 (bFGF) immunoreactivity in the substantia nigra of the rat

Cited by 51 publications

References 0 publications

Differential astroglial responses in the spinal cord of rats submitted to a sciatic nerve double crush treated with local injection of cultured Schwann cell suspension or lesioned spinal cord extract: implications on cell therapy for nerve repair

Differential astroglial responses in the spinal cord of rats submitted to a sciatic nerve double crush treated with local injection of cultured Schwann cell suspension or lesioned spinal cord extract: implications on cell therapy for nerve repair

Analysis of trophic responses in lesioned brain: focus on basic fibroblast growth factor mechanisms

S100ß and fibroblast growth factor-2 are present in cultured Schwann cells and may exert paracrine actions on the peripheral nerve injury

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