Corticosterone induced morphological changes of hippocampal and amygdaloid cell lines are dependent on 5-HT7 receptor related signal pathway

Xu, Ying; Zhang, Chao; Wang, R.; Govindarajan, Subramaniam S.; Barish, Philip A.; Vernon, Matthew M.; Fu, Chunhua; Acharya, Abhinav P.; Chen, L.; Boykin, Erin R.; Yu, Jia; Pan, Jianchun; O’Donnell, James M.; Ogle, William O.

doi:10.1016/j.neuroscience.2011.02.042

Cited by 30 publications

(13 citation statements)

References 51 publications

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“…Stress has been reported to alter the expression of several 5-HT receptor subtypes including 5HT 1A and 5-HT 7 receptors (Chaouloff et al, 1999, Chaouloff, 2000, Xu et al, 2011). Hence, we next examined the effects of repeated USS on anxiety-like behavior and looked to see if there were any correlated changes in the expression pattern of transcripts for the different 5-HT receptor subtypes in Type I – III neurons.…”

Section: Resultsmentioning

confidence: 99%

Differential distribution of serotonin receptor subtypes in BNSTALG neurons: Modulation by unpredictable shock stress

et al. 2012

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The bed nucleus of the stria terminalis (BNST) plays a critical role in regulating the behavioral response to stress. Stressors that activate the BNST also activate serotonergic (5-HT) systems. Hence, maladaptive changes of 5-HT receptor expression may contribute to stress-induced anxiety disorders. The BNST contains three neuronal types, Type I – III neurons. However, little is known about 5-HT receptor subtypes mRNA expression in these neurons, or whether it can be modulated by stress. Whole-cell patch clamp recording from Type I – III neurons was used in conjunction with single cell RT-PCR to characterize 5-HT receptor mRNA expression, and examine the effects of stress on this expression. We report that Type I neurons expressed mRNA transcripts predominantly for 5-HT1A and 5-HT7 receptors. Type II neurons expressed transcripts for every 5-HT receptor except the 5-HT2C receptor. Type II neurons were divided into three sub-populations: Type IIA in which transcripts for 5-HT3 and 5-HT7 receptors predominate, Type IIB that mainly express 5-HT1B and 5-HT4 receptor transcripts, and Type IIC in which transcripts for 5-HT1A and 5-HT2A receptors predominate. Type III neurons were also subdivided into two sub-populations; one that predominantly expressed transcripts for 5-HT1A, 5-HT1B and 5-HT2A receptors, and another that mainly expressed transcripts for 5-HT2C receptor. Unpredictable shock stress (USS) caused a long-lasting increase in anxiety-like behavior, and a concomitant decrease in 5-HT1A transcript expression in Type I – III neurons, as well as an up-regulation of a transcriptional repressor of 5-HT1A gene expression, deformed epidermal autoregulatory factor 1(Deaf-1). Significantly USS decreased 5-HT1A protein level, and increased the level of Deaf-1. USS also increased 5-HT1B transcript expression in Type III neurons, as well as 5-HT7 expression in Type I and II neurons. These data suggest that cell type-specific disruption of 5-HT receptor expression in BNSTALG neurons may contribute to stress-induced anxiety disorders.

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Section: Resultsmentioning

confidence: 99%

Differential distribution of serotonin receptor subtypes in BNSTALG neurons: Modulation by unpredictable shock stress

et al. 2012

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“…Cell cultures were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and cultured at 37 °C in 5% CO 2 51. Cells were plated at 2 × 10 5 cells/well on 6-well culture dishes and cultured overnight before treatment.…”

Section: Methodsmentioning

confidence: 99%

Comparison of the Pharmacological Profiles of Selective PDE4B and PDE4D Inhibitors in the Central Nervous System

Zhang

et al. 2017

Sci Rep

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Inhibition of cyclic AMP (cAMP)-specific phosphodiesterase 4 (PDE4) has been proposed as a potential treatment for a series of neuropsychological conditions such as depression, anxiety and memory loss. However, the specific involvement of each of the PDE4 subtypes (PDE4A, 4B and 4C) in different categories of behavior has yet to be elucidated. In the present study, we compared the possible pharmacological effects of PDE4B and PDE4D selective inhibitors, A-33 and D159687, in mediating neurological function in mice. Both compounds were equally potent in stimulating cAMP signaling in the mouse hippocampal cell line HT-22 leading to an increase in CREB phosphorylation. In contrast, A-33 and D159687 displayed distinct neuropharmacological effects in mouse behavioral tests. A-33 has an antidepressant-like profile as indicated by reduced immobility time in the forced swim and tail suspension tasks, as well as reduced latency to feed in the novelty suppressed feeding test. D159687, on the other hand, had a procognitive profile as it improved memory in the novel object recognition test but had no antidepressant or anxiolytic benefit. The present data suggests that inhibitors targeting specific subtypes of PDE4 may exhibit differential pharmacological effects and aid a more efficient pharmacotherapy towards neuropsychological conditions.

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“…To this end, HT-22 cells were treated with corticosterone and HCP1 expression was determined by both reverse-transcribed PCR (RT-PCR) and Western blot assays for mRNA and protein, respectively. Previous reports indicated that corticosterone impairs HT-22 cell viability at a concentration higher than 50 µM 38, 39 . Our results indicated that at as low as 15 µM, corticosterone treatment for 24 hours readily induced HCP1 mRNA transcription in HT-22 cells (Fig.…”

Section: Resultsmentioning

confidence: 89%

Physiological stress-induced corticosterone increases heme uptake via KLF4-HCP1 signaling pathway in hippocampus neurons

Zhang

Shen

Zhang

et al. 2017

Sci Rep

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Iron overload has attracted much attention because of its adverse effect in increasing the risk of developing several neurodegenerative disorders. Under various pathologic conditions, a lot of heme are released. The aggregation of heme is more neurotoxic than that of iron released from the heme breakdown. Our previous studies demonstrated that psychological stress (PS) is a risk factor of cerebral iron metabolism disorders, thus causing iron accumulation in rat brains. In the present study, we found PS could increase heme uptake via heme carrier protein 1 (HCP1) in rat brains. We demonstrated that Glucocorticoid (GC), which is largely secreted under stress, could up-regulate HCP1 expression, thus promoting heme uptake in neurons. We also ascertained that HCP1 expression can be induced by GC through a transcription factor, Krüppel-like factor 4 (KLF4). These results may gain new insights into the etiology of heme uptake and iron accumulation in PS rats, and find new therapeutic targets of iron accumulation in Parkinson’s disease or Alzheimer’s disease.

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Corticosterone induced morphological changes of hippocampal and amygdaloid cell lines are dependent on 5-HT7 receptor related signal pathway

Cited by 30 publications

References 51 publications

Differential distribution of serotonin receptor subtypes in BNSTALG neurons: Modulation by unpredictable shock stress

Differential distribution of serotonin receptor subtypes in BNSTALG neurons: Modulation by unpredictable shock stress

Comparison of the Pharmacological Profiles of Selective PDE4B and PDE4D Inhibitors in the Central Nervous System

Physiological stress-induced corticosterone increases heme uptake via KLF4-HCP1 signaling pathway in hippocampus neurons

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