Cost-Effective Recovery and Purification of Polyhydroxyalkanoates by Selective Dissolution of Cell Mass

Yu, Jian; Chen, Lilian X. L.

doi:10.1021/bp050362g

Cited by 105 publications

(87 citation statements)

References 21 publications

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“…The lowest amounts of biomass showed by soil isolate S3 that was 87 mg and 41 mg in NA and nutrient broth, respectively. [15,16] Soil isolate S4 showed 232 mg in NA, respectively. By considered other view of production standard PHB producer A. latus gave yields 370 mg of biomass which was greater than biomass yielded by other soil isolates organism hence that standard organism was selected for further production.…”

Section: Colony Characterizationmentioning

confidence: 99%

“…The plates and broth were incubated at 30°C for 48 h. [15] Harvesting of cell mass Cell mass from agar plates was scrapped with the help of spatula and that from broth 5 ml of 48 h at 30°C individual grown isolate was centrifuged at 6000 rpm for broth culture and 4000 rpm for solid state agar medium at 10°C; centrifugation time 15 min was employed for both medium, these processes were carried out in cooling centrifuge (Remi, Mumbai). Then, obtained cell pellets were suspended in deionized water.…”

Section: Figurementioning

confidence: 99%

“…The pellet of cell mass allows to air dry and after that placed in pre-weighed Petri plates dried to a constant weight at 60°C. [15,16] The plates were weighed again and dry cell weight of each isolate was calculated.…”

Section: Figurementioning

confidence: 99%

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Payghan

2016

IJGP

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Objective: There has been considerable interest in the development of biodegradable polymers such as poly-β-hydroxybutyrate (PHB) from bacterial origin which could help in solving probable problems due to the use of synthetic polymers. Many synthetic polymers are being used now-a-days in drug delivery systems. However, synthetic polymers have certain disadvantages such as their nonbiodegradability and so probability of bioaccumulation. Such accumulations for long time in body are harmful. This explains a need of easily biodegradable and a biocompatible polymer. Bacteria can synthesize a wide range of biopolymers which are biodegradable, biocompatible and have material properties suitable for medical applications. Bacterial polymers such as PHB are found to be functionally more effective and can be better for use in the pharmaceutical field. Materials and Methods: These studies started with screening of better producer of PHB from soil. This study represents a work on screening of bacterial isolates capable of producing PHB, and production of PHB using laboratory scale fermentation procedures. Using nutrient agar, we could screen four bacteria from soil, capable of producing PHB, using Sudan black-B staining. We also used a pure bacterial culture of Alcaligenes latus obtained from Microbial Type Culture Collection Chandigarh as a producer of PHB. Results: Out of these five bacterial isolates, A. latus found to produce PHB in relatively more amounts yielding about 55%. We used A. latus for production of PHB in large quantity. The morphological tests, physiochemical assessment, and characterizations of finished polymer were carried out. PHB shows viscosity 0.7993 (Pa.s), sharp peak arouse at 176.12°C in differential scanning calorimetry analysis, 235.8 nm λmax, successive Fourier transform infrared analysis, energy dispersive X-ray spectroscopy, and microscopic imaging analysis (scanning electron microscope [SEM]) were performed to get satisfactory reasoning for confirmation. Conclusion: PHB was used for formulating various formulations and was found to give good results, hence was concluded to be a better polymer in the pharmaceutical field.

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Section: Colony Characterizationmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

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Payghan

2016

IJGP

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“…PHA determination was conducted according to the Comeou et al (1988) and PHA concentration was determined according to the method of Braunegg et al (1998) using benzoic acid as internal standard. CDW was determined as described previously by Wong (2001), and synthesis of PHA was analysis according to the method of Yu & Chen (2006).…”

Section: Reactormentioning

confidence: 99%

“…Reduction of the molecular weight to some extent does not affect the mechanical properties of the biopolymers. The bioplastics, however, do become brittle or less ductile when the molecular weight is greatly reduced (e.g., <100-200 kDa) (Yu & Chen, 2006). PHA recovered by NPCM or dispersion digestion can change the molecular weight (M w ) of PHA.…”

Section: Molecular Weight Of Phamentioning

confidence: 99%

Microbial Biopolimerization Production from Palm Oil Mill Effluent (POME)

Ujang¹,

Salmiati²,

Salim³

2010

Biopolymers

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Pilot scale production and evaluation of mechanical and thermal properties of P(3HB) from Bacillus megaterium cultivated on desugarized sugar beet molasses

Schmid

Sykacek

O’Connor³

et al. 2021

J of Applied Polymer Sci

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Production of poly[(R)-3-hydroxyalkanoate] (PHA) biopolyesters must become more cost efficient in order to be competitive with conventional plastics as well as other bioplastics. This study introduces a simple and reproducible largescale production process of poly(3-hydroxybutyrate) P(3HB) with Bacillus megaterium based on desugarized sugar beet molasses and compares its material properties with commercially available types of PHA. A repeated batch cultivation system was evaluated in 1.2 L bioreactors and could be maintained for at least six cycles without a decrease in process stability, yielding a concentration of 20.4 g/L cell dry mass and 12 g/L P(3HB) per average process cycle. Feasibility of P(3HB) production was demonstrated at 500 L pilot scale to provide sufficient amounts of product for the determination of mechanical and thermal polymer properties. A volumetric productivity of 0.2 g L À1 h À1 was achieved.The resulting polymer exhibited a high tensile modulus of 3.73 GPa and tensile strength of 38.5 MPa. Despite a lower ductility and Vicat softening temperature, the properties of the recovered P(3HB) are comparable to commercial grade P(HB)HV polymers.

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Cost-Effective Recovery and Purification of Polyhydroxyalkanoates by Selective Dissolution of Cell Mass

Cited by 105 publications

References 21 publications

Untitled

Untitled

Microbial Biopolimerization Production from Palm Oil Mill Effluent (POME)

Pilot scale production and evaluation of mechanical and thermal properties of P(3HB) from Bacillus megaterium cultivated on desugarized sugar beet molasses

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