Cost-effective tissue culture media for large-scale propagation of three commercial banana (<i>Musa</i>spp.) varieties

Saraswathi, M.; Uma, S.; Kannan, G.; Selvasumathi, M.; Mustaffa, M. M.; Backiyarani, S.

doi:10.1080/14620316.2015.1117227

Cited by 27 publications

(15 citation statements)

References 12 publications

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“…Traditional genetic breeding has struggled to improve major agronomic traits and stress resistance in bananas, mainly due to its parthenocarpy and polyploidy (Ortiz and Swennen, 2014 ; Amah et al, 2019 ). Therefore, mutation breeding using in vitro propagated bananas has been proposed as a powerful approach to develop new cultivars or improved strains based on excellent cultivars (Karmarkar et al, 2001 ; Saraswathi et al, 2016 ; Datta et al, 2018 ). The mutagenic frequency of progenies can be increased to ca.…”

Section: Discussionmentioning

confidence: 99%

A Novel Banana Mutant “RF 1” (Musa spp. ABB, Pisang Awak Subgroup) for Improved Agronomic Traits and Enhanced Cold Tolerance and Disease Resistance

Wang

et al. 2021

Front. Plant Sci.

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Banana is a major fruit crop grown in tropical and subtropical regions worldwide. Among cultivars, “FenJiao, FJ” (Musa spp. ABB, Pisang Awak subgroup) is a popular variety of bananas, due to its better sugar-acid blend and relatively small fruit shape. However, because the traditional FJ variety grows relatively high in height, it is vulnerable to lodging and unsuitable for harvesting. In this study, we sought desirable banana mutants by carrying out ethyl methanesulfonate (EMS) mutagenesis with the FJ cultivar. After the FJ shoot tips had been treated with 0.8% (v/v) EMS for 4 h, we obtained a stably inherited mutant, here called “ReFen 1” (RF1), and also observed a semi-dwarfing phenotype. Compared with the wild type (FJ), this RF1 mutant featured consistently improved agronomic traits during 5-year field experiments conducted in three distinct locations in China. Notably, the RF1 plants showed significantly enhanced cold tolerance and Sigatoka disease resistance, mainly due to a substantially increased soluble content of sugar and greater starch accumulation along with reduced cellulose deposition. Therefore, this study not only demonstrated how a powerful genetic strategy can be used in fruit crop breeding but also provided insight into the identification of novel genes for agronomic trait improvement in bananas and beyond.

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Section: Discussionmentioning

confidence: 99%

A Novel Banana Mutant “RF 1” (Musa spp. ABB, Pisang Awak Subgroup) for Improved Agronomic Traits and Enhanced Cold Tolerance and Disease Resistance

Wang

et al. 2021

Front. Plant Sci.

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“…The success of this technique depends on various factors including explant selection, culture medium and culture conditions 12 – 14 . The high production cost prevents small- or medium-scale laboratories with limited resources from accessing the potential benefits of plant tissue culture technology 15 . Recent studies have been proposed to utilize automated temporary immersion systems (TIS), which is less expensive and provides an optimal environment for in vitro plant cell, tissue and organ cultures with good productivity 16 – 18 .…”

Section: Introductionmentioning

confidence: 99%

A novel temporary immersion bioreactor system for large scale multiplication of banana (Rasthali AAB—Silk)

Uma

Karthic

Kalpana

et al. 2021

Sci Rep

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Musa sp. cultivar Rasthali (Silk AAB) is a choice variety of the Asian sub-continent. Its production and sustenance are threatened by Fusarium wilt, which affects the livelihoods of small and marginal farmers. The use of quality planting material is one of the strategies to manage the disease. Availability of quality planting material for varieties other than Grand Naine is limited. Large-scale micropropagation using existing technologies is laborious and expensive. Temporary immersion bioreactor system is emerging as a potential advancement in the micropropagation industry. In this study, a cost-effective temporary immersion bioreactor (TIB) system has been developed and an efficient micropropagation method has been standardized. Explants cultured in TIB with 250 ml of culture medium in a 2-min immersion frequency of 6 h were found to be efficient for shoot proliferation and rooting. Its efficacy has been compared with the semisolid culture method. At the end of the 6th subculture, 1496 ± 110 shoots per explant were obtained in TIB. Chlorophyll, carotenoid, stomatal index, and the number of closed stomata were examined to determine the physiological functions of the plants grown in TIB and compared with semisolid grown plantlets. Plantlets grown in TIB were genetically stable and were confirmed using inter-simple sequence repeat (ISSR) markers. The multiplication of shoots in TIB was 2.7-fold higher than the semisolid culture method, which is suitable for large-scale production of planting material for commercial applications.

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“…Raghu et al (2007) reported that using tap water for tissue culture did not affect in vitro plant growth. Saraswathi et al (2016) reported that for banana micropropagation, three types of water (reverse osmosis water, drilled well water, and simple distilled water) were evaluated to reduce media preparation costs. Water costs were reduced by 86% by using inexpensive replacement water (reverse osmosis water, artesian well water) instead of double-distilled water.…”

Section: Low-cost Options For Distilled Watermentioning

confidence: 99%

“…However, bulla is less transparent, and contamination is difficult to detect (Ayenew et al, 2012;Mengesha et al, 2012;Ayalew et al, 2017). Similarly, other types of starches may exhibit such greening or darkening properties, e.g., Saraswathi et al (2016) reported that three gelling agents were used, and greening (10%-20%) was observed in a medium with corn flour.…”

Section: Low-cost Options For Gelling Agentmentioning

confidence: 99%

“…Similarly, Isabgolbased medium enhanced the initial establishment of shoot tips in 'Udhayam' and 'Rasthali' banana cultivars, and the combination of Sago and Isabgol was found to be a better gelling agent for many crops, including banana. The effectiveness of these alternative gelling agents might have been due to their high mucilage content (>30%) or their polysaccharide and colloidal nature (Saraswathi et al, 2016).…”

Section: Low-cost Options For Gelling Agentmentioning

confidence: 99%

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Plant tissue culture challenges in Ethiopia and alternative options for low-cost media

Amare

Dugassa²

2022

F1000Res

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Plant tissue culture (PTC) is the cultivation of any part of a plant in nutritionally defined media under an aseptic and controlled environment, regardless of season and weather. The application of PTC leads to the mass propagation of varietal, high-quality seedlings of ornamental plants, medicinal plants, plantation crops, fruit trees, and forest trees. PTC technology, on the other hand, is more expensive in developing nations, such as Ethiopia, than traditional propagation methods such as seeds, cuttings, grafting, and so on. As a result, it is critical to take steps to cut production costs and explore alternate choices for present PTC obstacles (budget restrictions, procedural and operational matters, and unfortunate interactions and partnerships). In order to lower the unit cost of crop production, cost-effective procedures and the optimal utilization of equipment are required. This can be accomplished by increasing the efficiency of processes and optimizing resource allocation. Gelling agents, macro and micronutrients, equipment, carbon sources, and the utilization of bioreactors, which can minimize space, energy, and labor needs, can all be replaced to lower production costs. Therefore, these alternative options are recommended as a workaround to the problems and are briefly described in this document.

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Cost-effective tissue culture media for large-scale propagation of three commercial banana (Musaspp.) varieties

Cited by 27 publications

References 12 publications

A Novel Banana Mutant “RF 1” (Musa spp. ABB, Pisang Awak Subgroup) for Improved Agronomic Traits and Enhanced Cold Tolerance and Disease Resistance

A Novel Banana Mutant “RF 1” (Musa spp. ABB, Pisang Awak Subgroup) for Improved Agronomic Traits and Enhanced Cold Tolerance and Disease Resistance

A novel temporary immersion bioreactor system for large scale multiplication of banana (Rasthali AAB—Silk)

Plant tissue culture challenges in Ethiopia and alternative options for low-cost media

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