Cotton fabric as an immobilization matrix for low-cost and quick colorimetric enzyme-linked immunosorbent assay (ELISA)

Abstract

Cotton-fabric based 2-dimensional colorimetric ELISA which requires a smaller amount of sample and reagents, with quicker assay time.

Electrochemiluminescence detection in microfluidic cloth-based analytical devices

“…Up to now, several methods have been well developed for fabrication of μCADs, including weaving (Bhandari et al, 2011;Owens et al, 2011;Vatansever et al, 2012), batik painting (Nilghaz et al, 2012(Nilghaz et al, , 2015Bagherbaigi et al, 2014;Malon et al, 2014), stereo-stitching (Xing et al, 2013), wax screen-printing (Guan et al, 2015), and photolithography (Wang et al, 2014;Baysal et al, 2014aBaysal et al, , 2014bBaysal et al, , 2015Wu and Zhang, 2015). The as-manufactured μCADs combine the simplicity of cloth strip tests and the complexity of the conventional lab-on-chip devices, and as a result become a class of microfluidic systems.…”

“…The as-manufactured μCADs combine the simplicity of cloth strip tests and the complexity of the conventional lab-on-chip devices, and as a result become a class of microfluidic systems. Moreover, these μCADs are capable of performing a series of biochemical assays or other applications, such as immunoassay (Bhandari et al, 2011;Bagherbaigi et al, 2014), detection of glucose or protein in artificial urine (AU) (Nilghaz et al, 2012(Nilghaz et al, , 2015Wu and Zhang, 2015), assay of hydrogen peroxide (H 2 O 2 ) (Baysal et al, 2015;Guan et al, 2015), lactate measurement in saliva (Malon et al, 2014) or phosphate buffer solution (PBS) (Baysal et al, 2014a(Baysal et al, , 2014b, removal of sweat on an artificial skin surface (Xing et al, 2013), pH sensitive liquid transport (Vatansever et al, 2012), and liquid-liquid extraction (Owens et al, 2011).…”

“…Possibly because of its simplicity, colorimetric detection is the most used method in μCADs (Bhandari et al, 2011;Nilghaz et al, 2012Nilghaz et al, , 2015Bagherbaigi et al, 2014;Baysal et al, 2014aBaysal et al, , 2014bBaysal et al, , 2015Wu and Zhang, 2015), where specific reagents are applied to the device and the developed color intensity and/or hue correlates with analyte concentration. In general, however, only qualitative or semiquantitative measurement can be obtained.…”

Monitoring magnesium degradation using microdialysis and fabric-based biosensors

“…They were polished using abrasive paper grit # 1200, ultrasonically cleaned in ethanol at 30°C for 10 min, etched with 2% nital solution to remove oxides, and finally rinsed with distilled water before drying in the desiccator for an hour. A fabric-based electrochemical device (FED) was used as described previously [30,31] entrapment of enzymes within the fibers without requiring additional artificial membranes or chemicals for their immobilization [32]. Briefly, the fabrication of FED consists of a white plain cotton fabric (Jadi Batek Gallery, Malaysia) that was previously scoured using anhydrous sodium carbonate [30].…”

A low-cost, ultraflexible cloth-based microfluidic device for wireless electrochemiluminescence application

“…We and others have developed several fabrication methods for μCADs, including weaving [8,9,11,12], cutting [13], batik painting [6,7,14,15], stereo-stitching [10], wax screen-printing [16][17][18], and photolithography [19][20][21][22][23]. Cloth is a viable material for the development of analytical devices due to its low material and manufacture costs, ability to wick assay fluids by capillary forces, and potential for patterning multiplexed channel geometries.…”