Cotton <i>GhSSI2</i> isoforms from the stearoyl acyl carrier protein fatty acid desaturase family regulate Verticillium wilt resistance

Mo, Shaojing; Zhang, Yan; Wang, Xingfen; Yang, Jun‐Bo; Sun, Zhengwen; Zhang, Dongmei; Chen, Bin; Wang, Guoning; Ke, Huifeng; Liu, Zhengwen; Meng, Chengsheng; Li, Zhikun; Wu, Liqiang; Zhang, Guiyin; Duan, Huijun; Ma, Zhiying

doi:10.1111/mpp.13093

Cited by 21 publications

(21 citation statements)

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“…Compared with the WT, more resistance genes were activated in GhENODL transgenic plants, and KEGG revealed phenylpropanoid biosynthesis was enriched ( Figure 7 b). Given the fact that the phenylalanine ammonia lyase pathway is the main synthesis pathway of SA in cotton [ 35 ] and the results of qPCR in Figure 4 e and the RNA-seq data, we deduced that GhENODL6 is probably involved in SA signaling. Thus, the SA pathway-regulated genes, EDS1, PR1, PR2, PR5, and PR10 , were detected via qPCR ( Figure 8 a–e).…”

Section: Resultsmentioning

confidence: 78%

“…SA is a key defense-related hormone in plants, which plays an important role in SAR and broad-spectrum durable disease resistance Among the reported plant defense hormones, the roles of SA, JA, and ethylene (Eth) have been established, and these hormones play important roles in biological stress signaling pathways, including ROS and MAPK kinases [8]. In cotton, most SA biosynthesis relies on the phenylalanine ammonia lyase pathway [35]. In this study, according to qPCR results and RNA-seq data, we inferred that GhENODL6 could activate the phenylpropanoid pathway and promote SA synthesis.…”

Section: Discussionmentioning

confidence: 99%

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GhENODL6 Isoforms from the Phytocyanin Gene Family Regulated Verticillium Wilt Resistance in Cotton

Zhang

Wang

Yang

et al. 2022

IJMS

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Verticillium wilt (VW), a fungal disease caused by Verticillium dahliae, currently devastates cotton fiber yield and quality seriously, yet few resistance germplasm resources have been discovered in Gossypium hirsutum. The cotton variety Nongda601 with suitable VW resistance and high yield was developed in our lab, which supplied elite resources for discovering resistant genes. Early nodulin-like protein (ENODL) is mainly related to nodule formation, and its role in regulating defense response has been seldom studied. Here, 41 conserved ENODLs in G. hirsutum were identified and characterized, which could divide into four subgroups. We found that GhENODL6 was upregulated under V. dahliae stress and hormonal signal and displayed higher transcript levels in resistant cottons than the susceptible. The GhENODL6 was proved to positively regulate VW resistance via overexpression and gene silencing experiments. Overexpression of GhENODL6 significantly enhanced the expressions of salicylic acid (SA) hormone-related transcription factors and pathogenicity-related (PR) protein genes, as well as hydrogen peroxide (H2O2) and SA contents, resulting in improved VW resistance in transgenic Arabidopsis. Correspondingly, in the GhENODL6 silenced cotton, the expression levels of both phenylalanine ammonia lyase (PAL) and 4-coumarate-CoA ligase (4CL) genes significantly decreased, leading to the reduced SA content mediating by the phenylalanine ammonia lyase pathway. Taken together, GhENODL6 played a crucial role in VW resistance by inducing SA signaling pathway and regulating the production of reactive oxygen species (ROS). These findings broaden our understanding of the biological roles of GhENODL and the molecular mechanisms underlying cotton disease resistance.

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Section: Resultsmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

GhENODL6 Isoforms from the Phytocyanin Gene Family Regulated Verticillium Wilt Resistance in Cotton

Zhang

Wang

Yang

et al. 2022

IJMS

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“…Furthermore, our previous iTRAQ-based quantitative proteomic study showed a more significant accumulation of PAL protein (Cla008727) in pumpkin rootstock-grafted than self-grafted watermelon plants after exposure to chilling for 48 h [ 45 ]. The virus-induced gene silencing of PAL in cotton plants showed reduced levels of both free SA and SAG content, suggesting that the SA biosynthesis is critically dependent on the PAL pathway [ 46 ]. Many chemical modifications of SA can occur in cells, and glucose conjugation at the hydroxyl group of SA leads to the biosynthesis of inactive SAG which is stored in the vacuolar [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Spatial–Temporal Response of Reactive Oxygen Species and Salicylic Acid Suggest Their Interaction in Pumpkin Rootstock-Induced Chilling Tolerance in Watermelon Plants

Cheng

Gao

et al. 2021

Antioxidants

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Grafting with pumpkin rootstock could improve chilling tolerance in watermelon, and salicylic acid (SA) as a signal molecule is involved in regulating plant tolerance to chilling and other abiotic stresses. To clarify the mechanism in pumpkin rootstock-induced systemic acquired acclimation in grafted watermelon under chilling stress, we used self-grafted (Cl/Cl) and pumpkin rootstock-grafted (Cl/Cm) watermelon seedlings to study the changes in lipid peroxidation, photosystem II (PSII) activity and antioxidant metabolism, the spatio–temporal response of SA biosynthesis and H2O2 accumulation to chilling, and the role of H2O2 signal in SA-induced chilling tolerance in grafted watermelon. The results showed that pumpkin rootstock grafting promoted SA biosynthesis in the watermelon scions. Chilling induced hydrolysis of conjugated SA into free SA in the roots and accumulation of free SA in the leaves in Cl/Cm plants. Further, pumpkin rootstock grafting induced early response of antioxidant enzyme system in the roots and increased activities of ascorbate peroxidase and glutathione reductase in the leaves, thus maintaining cellular redox homeostasis. Exogenous SA improved while the inhibition of SA biosynthesis reduced chilling tolerance in Cl/Cl seedlings. The application of diphenyleneiodonium (DPI, inhibitor of NADPH oxidase) and dimethylthiourea (DMTU, H2O2 scavenger) decreased, while exogenous H2O2 improved the PSII activity in Cl/Cl plants under chilling stress. Additionally, the decrease of the net photosynthetic rate in DMTU- and DPI-pretreated Cl/Cl plants under chilling conditions could be alleviated by subsequent application of H2O2 but not SA. In conclusion, pumpkin rootstock grafting induces SA biosynthesis and redistribution in the leaves and roots and participates in the regulation of antioxidant metabolism probably through interaction with the H2O2 signal, thus improving chilling tolerance in watermelon.

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“…A moisturizing transparent high cover was used to protect the pots for 24 h to prevent evaporation. The necrotic spots in the leaves were observed with trypan blue staining [ 85 ]. Plants treated with 10 μM TA were prepared for protein extraction because this concentration of TA effectively induced a leaf response.…”

Section: Methodsmentioning

confidence: 99%

iTRAQ-Based Proteomics Analysis of Response to Solanum tuberosum Leaves Treated with the Plant Phytotoxin Thaxtomin A

Liu

Hao

Liu

et al. 2021

IJMS

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Thaxtomin A (TA) is a phytotoxin secreted by Streptomyces scabies that causes common scab in potatoes. However, the mechanism of potato proteomic changes in response to TA is barely known. In this study, the proteomic changes in potato leaves treated with TA were determined using the Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technique. A total of 693 proteins were considered as differentially expressed proteins (DEPs) following a comparison of leaves treated with TA and sterile water (as a control). Among the identified DEPs, 460 and 233 were upregulated and downregulated, respectively. Based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, many DEPs were found to be involved in defense and stress responses. Most DEPs were grouped in carbohydrate metabolism, amino acid metabolism, energy metabolism, and secondary metabolism including oxidation–reduction process, response to stress, plant–pathogen interaction, and plant hormone signal transduction. In this study, we analyzed the changes in proteins to elucidate the mechanism of potato response to TA, and we provided a molecular basis to further study the interaction between plant and TA. These results also offer the option for potato breeding through analysis of the resistant common scab.

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Cotton GhSSI2 isoforms from the stearoyl acyl carrier protein fatty acid desaturase family regulate Verticillium wilt resistance

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 21 publications

References 90 publications

GhENODL6 Isoforms from the Phytocyanin Gene Family Regulated Verticillium Wilt Resistance in Cotton

GhENODL6 Isoforms from the Phytocyanin Gene Family Regulated Verticillium Wilt Resistance in Cotton

Spatial–Temporal Response of Reactive Oxygen Species and Salicylic Acid Suggest Their Interaction in Pumpkin Rootstock-Induced Chilling Tolerance in Watermelon Plants

iTRAQ-Based Proteomics Analysis of Response to Solanum tuberosum Leaves Treated with the Plant Phytotoxin Thaxtomin A

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