Covalent immobilization of chitosan and heparin on PLGA surface

The effect of coimmobilizing heparin and fibronectin on titanium on hemocompatibility and endothelialization

“…In contrast, covalent or ionic immobilization of biomolecules has the advantages that the method is easy to be realized and the immobilized biomolecules are not easy to be removed by rinsing and robust enough to withstand in vivo exposure [12,37], though the bioactivity of the biomolecules may be influenced, which, however, could be improved by change the immobilization conditions. To our best knowledge, biomaterials coimmobilized with different biomolecules can possess the properties that each biomolecule has [17]. The immobilization of different biomolecules on biomaterial surfaces has been proven to improve blood compatibility [9] or enhance cell attachment and proliferation [12].…”

“…Few studies have been found to investigate the both aspects simultaneously [15,16]. Though the previous studies have shown that anticoagulation and endothelialization simultaneously are contradictory, to our best study, biomaterials coimmobilized with different biomolecules can possess the properties of each individual biomolecule [17]. Thus the biomaterials surface simultaneously immobilized with different biomolecules possessing anticoagulant and pro-endothelialization properties, respectively, is hypothesized to improve both aspects of anticoagulation and endothelialization, which may provide a potential application for long-term cardiovascular implants, though there seem to be only few studies in this direction [18].…”

Immobilization of bovine serum albumin on TiO2 film via chemisorption of H3PO4 interface and effects on platelets adhesion

“…Silanization was done in the solvent of toluene, not in ethanol or acetone, because it can be resulted in high surface density of amino group on the surface [10][11][12]. N-hydroxysuccinimide (NHS) was used to minimize the hydrolysis of EDC [13][14].…”

Immobilization of Heparin: Approaches and Applications

“…Wang and coworkers achieved the immobilization of heparin on PLGA surfaces through EDC/NHS coupling (Fig. 26) [48]. PLGA film was prepared by pouring a PLGA solution (75:25) in chloroform on a watch glass and drying the chloroform over a current a dry air for 24 h. Thus obtained PLGA film was cross linked with chitosan (0.2 %) in EDC/NHS/MES for 4 h. Heparinization of chitosan cross-linked PLGA surface was achieved by immersing in 1 % heparin solution for 4 h followed by extensive washing with water.…”