Covalently coating dextran on macroporous polyglycidyl methacrylate microsphere enabled rapid protein chromatographic separation

Zhang, Rongyue; Li, Qiang; Li, Juan; Zhou, Weiqing; Ye, Peili; Gao, Yang; Ma, Guanghui; Su, Zhiguo

doi:10.1016/j.msec.2012.08.021

Cited by 16 publications

(3 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The macroporous poly(GMA-co-EGDMA) microspheres were prepared by the surfactant reverse micelle swelling method, which is an easy and novel method of preparing macroporous microspheres with a pore size of 140-200 nm. 20 In comparison with the ST-co-DVB 17 and GMA-co-DVB 30 systems, the lower hydrophobicity of the GMA-co-EGDMA system led to microspheres with a smaller pore size, which weakened the separation between the water-surfactant phase and the polymer phase. However, we found that microspheres with a larger pore size were obtained by changing the amount of EGDMA (40 wt % of monomer phase) and increasing the amount of Span80 (27.5 wt % of monomer phase) and Na 2 SO 4 (0.05 wt % of aqueous phase), in which microspheres with a maximum pore size of around 370 nm and particle size in the range 20-50 μm were obtained.…”

Section: Synthesis Of the Functionalized Microspheresmentioning

confidence: 99%

Poly(aspartic acid) surface modification of macroporous poly(glycidyl methacrylate) microspheres

Wenwei

Luo

et al. 2019

J of Applied Polymer Sci

View full text Add to dashboard Cite

Novel macroporous, hydrophilic microspheres with a surface layer of crosslinked poly(aspartic acid) were synthesized. In this study, macroporous poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) [poly(GMA-co-EGDMA)] microspheres with pore size around 370 nm were first obtained through the surfactant reverse micelle swelling method, and the poly(GMA-co-EGDMA) was aminated by ethylene diamine to form poly(GMA-NH 2 ). The polysuccinimide was grafted onto the surface of poly(GMA-NH 2 ) microspheres and crosslinked by hexamethylendiamine and γ-aminopropyltriethoxysilane, respectively, and then hydrolyzed to obtain the poly(aspartic acid)-functionalized macroporous microspheres. The functionalized hydrophilic microspheres were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis, mercury porosimetry, and elemental analysis. The metal ion adsorption capacity was also studied. The FTIR, XPS, and elemental analysis confirmed the poly(aspartic acid) functionalization of the poly(GMA-co-EGDMA) microspheres. SEM and mercury porosimetry showed there was little effect of this surface chemical modification on microsphere porosity, and the obtained macroporous microspheres exhibited excellent thermal stability and adsorption for Ag(I), presenting great potential for applications in adsorption, fixation, and separation.

show abstract

Section: Synthesis Of the Functionalized Microspheresmentioning

confidence: 99%

Poly(aspartic acid) surface modification of macroporous poly(glycidyl methacrylate) microspheres

Wenwei

Luo

et al. 2019

J of Applied Polymer Sci

View full text Add to dashboard Cite

show abstract

“…Last but not least, they must possess minimal affinity to compounds other than the separated ones, that is, they must ensure minimal nonspecific interactions. Minimization of the nonspecific adsorption onto the particle surface can be achieved by modification with various polymers, for example, gelatin, dextran, albumin, poly( N ‐isopropylacrylamide) (PNIPAm), and most frequently poly(ethylene glycol) . The ability to resist nonspecific protein adsorption depends not only on the character of the polymer used for the modification but also on its molecular weight (degree of polymerization) and the quality of the surface coverage (graft density) .…”

Section: Introductionmentioning

confidence: 99%

Raft polymerization ofN,N-dimethylacrylamide from magnetic poly(2-hydroxyethyl methacrylate) microspheres to suppress nonspecific protein adsorption

Cao

Horák

et al. 2015

J. Polym. Sci. Part A: Polym. Chem.

View full text Add to dashboard Cite

Nonspecific interaction is a key parameter affecting the efficiency of proteins, nucleic acids or cell separation. Currently, many approaches to introduce antifouling properties to materials have been developed. Among these, surface modification with polymer brushes plays a prominent role. The aim of this study was to synthesize new magnetic microspheres grafted with poly(N,N‐dimethylacrylamide) (PDMA) that resist nonspecific protein adsorption. Monodisperse macroporous poly(2‐hydroxyethyl methacrylate) (PHEMA) microspheres, 4 μm in size, were synthesized by a multiple swelling polymerization method. To render the microspheres magnetic, iron oxide was precipitated inside the microsphere pores. Functional carboxyl groups, introduced by the hydrolysis of the 2‐(methacryloyl)oxyethyl acetate (HEMA‐Ac) comonomer, were used to react with propargylamine, followed by coupling of a chain transfer agent via an azide‐alkyne click reaction. PDMA was grafted from the PHEMA microspheres using reversible addition‐fragmentation chain transfer polymerization (RAFT), resulting in surfaces with more than 81 wt % PDMA attached. The successful modification of the microspheres was confirmed by XPS. The magnetic microspheres grafted with PDMA showed excellent antifouling properties as tested in bovine serum protein solutions. © 2015 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016, 54, 1036–1043

show abstract

“…Different routes for coating macroporous supports with organic and inorganic materials have been explored in recent years. [1][2][3] The materials coated onto macroporous supports include extended networks such as metal-organic frameworks (MOFs), which are synthesized by the self-assembly of metal ions and an organic part, at temperatures ranging from room temperature to 250 C. [4][5][6] MOFs have desirable characteristics such as high surface area, and high structural and functional diversity. 6,7 To improve their ease of handling and efficiency, they are prepared as MOF composites where the MOFs are grown on a variety of substrates, including macroporous polymers.…”

Section: Introductionmentioning

confidence: 99%

Reversible in situ precipitation: a flow-through approach for coating macroporous supports with metal hydroxides

2014

View full text Add to dashboard Cite

Covalently coating dextran on macroporous polyglycidyl methacrylate microsphere enabled rapid protein chromatographic separation

Cited by 16 publications

References 35 publications

Poly(aspartic acid) surface modification of macroporous poly(glycidyl methacrylate) microspheres

Poly(aspartic acid) surface modification of macroporous poly(glycidyl methacrylate) microspheres

Raft polymerization ofN,N-dimethylacrylamide from magnetic poly(2-hydroxyethyl methacrylate) microspheres to suppress nonspecific protein adsorption

Reversible in situ precipitation: a flow-through approach for coating macroporous supports with metal hydroxides

Contact Info

Product

Resources

About