Covariation patterns of phytoplankton and bacterioplankton in hypertrophic shallow lakes

Abstract

The aim of this work was to assess the temporal patterns in the community composition of phytoplankton (PCC) and bacterioplankton (BCC) in two interconnected and hypertrophic Pampean shallow lakes (Argentina). Factors shaping their community dynamics and community temporal covariations were also analysed. We performed 4 years of seasonal samplings (2012–2016) and communities were studied by Utermöhl approach (PCC) and Illumina MiSeq sequencing (BCC). We found marked seasonal variations in both communities, and inter-annual variations with decreasing microbial community similarities along the study. We also observed covariation in community-level dynamics among PCC and BCC within- and between-shallow lakes. The within-lake covariations remained positive and significant, while controlling for the effects of intrinsic (environmental) and extrinsic (temporal and meteorological) factors, suggesting a community coupling mediated by intrinsic biotic interactions. Algal-bacterial associations between different taxa of phytoplankton and bacterioplankton within each lake were also found. PCC was mainly explained by pure regional extrinsic (17–21%) and intrinsic environmental (8–9%) factors, while BCC by environmental (8–10%) and biotic interactions with phytoplankton (7–8%). Our results revealed that the influence of extrinsic regional factors can be channeled to bacterioplankton through both environmental (i.e. water temperature) and phytoplankton effects.

Temporal dynamics of Legionella (Proteobacteria, Legionellaceae) in two Pampean shallow lakes from Argentina

“…Additionally, Gómez shallow lake is used for bathing and swimming during spring and summer seasons. A comprehensive and detailed characterization, including principal physical, chemical and biological parameters, has been conducted by Quirós and Drago(Quirós and Drago 1999) and Schia no et al (Schia no et al 2019(Schia no et al , 2020.…”

Estructura y dinámica del bacterioplancton en cuatro lagunas pampeanas de la cuenca del río Salado (Buenos Aires, Argentina)

“…En particular, pocos trabajos en las lagunas asociadas al río Salado estudiaron la dinámica y la estructura del bacterioplancton utilizando herramientas moleculares. La mayor parte se realizaron en lagunas de la cuenca inferior del río Salado (Llames 2011;Llames et al 2013Llames et al , 2017Sánchez et al 2015; Schiaffino et al 2020). Sin embargo, este trabajo es el primero en abordar un análisis comparativo de las comunidades del bacterioplancton y su diversidad genética entre lagunas pertenecientes a la cuenca superior e inferior del río Salado, las cuales presentan impactos antrópicos diferenciales.…”

“…Además, se midió in situ la profundidad en el sitio de muestreo, el pH, la conductividad eléctrica, la temperatura, el oxígeno disuelto (OD) y la turbidez nefelométrica mediante el uso de sensores de campo (HANNA® pH/cond meter HI991301, HANNA® HI9146 y HACH® 2100P Turbidimeter), y la profundidad del disco de Secchi. Para estudiar el bacterioplancton, las muestras se prefiltraron a través de una E� ���������������� �� ������� ��������� ₃₄₇ membrana de 55 μm de poro, luego filtradas por filtros de 0.2 μm (Millipore™ GTTP) y almacenadas a -80 °C (Schiaffino et al 2020).…”

Dynamics of associated microbiomes during algal bloom development: to see and to be seeing

“…The mechanisms of how bacteria alter the phycosphere and interactions between phytoplankton and bacteria are unclear. Changes in the composition of the phytoplankton community have been shown to correlate with changes in bacterial community composition 20,21 . Algal-bacterial interactions can manifest not only as mutualistic 1,9,22,23 but also as competition over limited inorganic resources 8,24 .…”

“…The mechanisms of how bacteria alter the phycosphere and interactions between phytoplankton and bacteria are unclear. Changes in the composition of the phytoplankton community have been shown to correlate with changes in bacterial community composition 20,21 .…”