Coverage Control of DNA Crystals Grown by Silica Assistance

Lee, Junwye; Kim, Sun-Ho; Kim, Jung-Hoon; Lee, Chang-Won; Roh, Yonghan; Park, Sungha

doi:10.1002/anie.201103604

Cited by 35 publications

(36 citation statements)

References 28 publications

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“…This work shows that the bulk Mg 2 þ concentration cannot be used as the sole predictor of the behaviour of DNA origami on mica. In fact, the phenomena observed in this work cannot be observed by simply varying Mg 2 þ concentration, as previous works studying the surface-mediated assembly of small DNA tiles have done 28 . Further this work demonstrates that one cannot simply translate models and results achieved for linear dsDNA to artificial DNA nanostructures since DNA size and geometry have profound effects on DNA-mica binding.…”

Section: Discussionmentioning

confidence: 76%

“…It is fast, simple and unlike previous surface-based self-assembly of small DNA tiles [26][27][28] our method can operate isothermally, which may be advantageous if the method is used to organize temperaturesensitive components such as proteins.…”

Section: Discussionmentioning

confidence: 99%

“…Assembling DNA lattices directly on substrates may provide a route to more reproducible patterning. Several studies have demonstrated that the annealing of small DNA tiles over mica or silicon dioxide can form large lattices [26][27][28] , but these lattices are comprised of B10 nm tiles, which cannot be used to template complex multi-component devices. Such protocols are incompatible with origami because, under the divalent-cation-rich conditions used, origami (which are much larger than the tiles) are immobile and cannot diffuse to form lattices.…”

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confidence: 99%

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Self-assembly of two-dimensional DNA origami lattices using cation-controlled surface diffusion

2014

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DNA origami has proven useful for organizing diverse nanoscale components into patterns with 6 nm resolution. However for many applications, such as nanoelectronics, large-scale organization of origami into periodic lattices is desired. Here, we report the self-assembly of DNA origami rectangles into two-dimensional lattices based on stepwise control of surface diffusion, implemented by changing the concentrations of cations on the surface. Previous studies of DNA-mica binding identified the fractional surface density of divalent cationsñ s2 ð Þ as the parameter which best explains the behaviour of linear DNA on mica. We show that for n s2 between 0.04 and 0.1, over 90% of DNA rectangles were incorporated into lattices and that, compared with other functions of cation concentration,ñ s2 best captures the behaviour of DNA rectangles. This work shows how a physical understanding of DNA-mica binding can be used to guide studies of the higher-order assembly of DNA nanostructures, towards creating large-scale arrays of nanodevices for technology.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Self-assembly of two-dimensional DNA origami lattices using cation-controlled surface diffusion

2014

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“…The recently developed annealing method called SAG242526 uses a different fabrication method than the conventional free solution annealing method. For SAG processes, a negatively charged substrate is immersed into physiological DNA buffer solution.…”

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confidence: 99%

“…Through electrostatic interaction between negatively charged DNA molecules and the substrate mediated by divalent magnesium ions, DNA crystals are formed on the substrate during the annealing process. DNA Crystals grown by SAG methods show somewhat different topologies, including the aspect ratio and dimensionality242526. In addition, accurate coverage control of DNA crystals onto substrate can be achieved from 0 to 100% at relatively low DNA concentrations26.…”

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confidence: 99%

Quantitative analysis of molecular-level DNA crystal growth on a 2D surface

Lee

Hamada

Hwang

et al. 2013

Sci Rep

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Crystallization is an essential process for understanding a molecule's aggregation behavior. It provides basic information on crystals, including their nucleation and growth processes. Deoxyribonucleic acid (DNA) has become an interesting building material because of its remarkable properties for constructing various shapes of submicron-scale DNA crystals by self-assembly. The recently developed substrate-assisted growth (SAG) method produces fully covered DNA crystals on various substrates using electrostatic interactions and provides an opportunity to observe the overall crystallization process. In this study, we investigated quantitative analysis of molecular-level DNA crystallization using the SAG method. Coverage and crystal size distribution were studied by controlling the external parameters such as monomer concentration, annealing temperature, and annealing time. Rearrangement during crystallization was also discussed. We expect that our study will provide overall picture of the fabrication process of DNA crystals on the charged substrate and promote practical applications of DNA crystals in science and technology.

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Environment‐Dependent Self‐Assembly of DNA Origami Lattices on Phase‐Separated Lipid Membranes

Sato

Endo

Morita

et al. 2018

Adv Materials Inter

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Self‐assembly of designed nanostructures on a lipid membrane offers a powerful approach for enhancing the functionality of its surface. Here, the environment‐dependent assembly of blunt‐ended DNA origami structures on a phase‐separated lipid‐bilayer membrane consisting of liquid‐disordered (Ld) and solid‐ordered (So) phases is reported. Fluorescence microscopy and atomic force microscopy (AFM) imaging reveal that DNA origami structures preferentially bind to the So phase. At higher concentrations, blunt‐ended origamis form 2D lattices on the Ld phase through surface‐mediated self‐assembly. In contrast, those adsorbed on the So phase are less mobile and pack into aggregates. However, this situation is changed by adding NaCl. High‐speed AFM imaging reveals the mechanism underlying the NaCl‐induced changes, wherein the lattices on the Ld phase desorb from the surface while the packed aggregates on the So phase reorganize into the lattices. These results indicate that the formation of 2D lattices depends on the phase of the lipids; however, it can be tuned by ionic conditions, enabling us to select the domain on which the lattice is formed. The present results may guide the creation of membrane‐supported DNA nanostructures that self‐assemble into functional states in a lipid phase or ion‐responsive manner.

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Coverage Control of DNA Crystals Grown by Silica Assistance

Cited by 35 publications

References 28 publications

Self-assembly of two-dimensional DNA origami lattices using cation-controlled surface diffusion

Self-assembly of two-dimensional DNA origami lattices using cation-controlled surface diffusion

Quantitative analysis of molecular-level DNA crystal growth on a 2D surface

Environment‐Dependent Self‐Assembly of DNA Origami Lattices on Phase‐Separated Lipid Membranes

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