Crab Shell Extract Improves Serum Biochemical Markers and Histological Changes of Pancreas in Diabetic Rats

Abstract

MAKALANI, F.; KHAZAEI, M. R.; GHANBARI, E. & KHAZAEI, M.Crab shell extract improves serum biochemical markers and histological changes of pancreas in diabetic rats. Int. J. Morphol., 35(4):1437Morphol., 35(4): -1443Morphol., 35(4): , 2017 SUMMARY: Diabetes mellitus is a common metabolic disease. There are many natural agents available to control and treat diabetes. Crab shell extract has antioxidant properties. The aim of present study was to investigate the effect of crab shell hydroalcoholic extract on blood glucose, liver enzymes, nitric oxide and antioxidant capacity of serum and histological structure of pancreas in diabetic rats. In this experimental study, thirty five male Wistar rats (180-220 g) were divided into control, diabetic and experimental groups (n=7). Diabetes was induced by intraperitoneal injection of streptozotocin (60 mg/kg). Rats were treated for 14 days by crab shell extract with 100, 200 and 400 mg/kg doses. Fasting blood glucose, serum levels of liver enzymes, nitric oxide (NO) and total antioxidant capacity were evaluated. Changes of pancreatic tissue were determined using a modified aldehyde fuchsin staining method. Data were analyzed using one-way ANOVA. Differences were considered statistically significant at P<0.05. Crab shell extract induced a significant reduction in blood glucose, serum levels of nitric oxide and ALT (P=0.033). Also, there were a significant increase in total antioxidant capacity (FRAP) (P=0.007), and insignificant decrease in serum levels of AST. The extract improved pancreatic tissue changes caused by diabetes. In conclusion, antioxidant and anti-diabetic effects of crab shell increase total antioxidant capacity of serum and decreased blood glucose, serum nitric oxide and ALT levels.

The Possible Protective Role of Powder Cuttlefish Bone, Crabshell and Eggshell on Osteoporotic Rats

“…Additionally, Ramasamy et al, (2014) found that rats treated with chitosan against CCl 4 poisonous quality indicated significantly decreased levels of ALT and AST exercises, total cholesterol, triglyceride and free unsaturated fat in plasma and tissue. These results were according to Makalani et al, (2017) who reported that crab shell expel affected an imperative decline in serum levels of ALT and AST.…”

“…Selenium is a basic piece of the human eating regimen and is found in crab shell (Brozmanová et al, 2010). Likewise, selenium has cell reinforcement and mitigating impacts (Akhuemokhan et al, 2013 andMakalani et al, 2017).…”

Renal Tissue Damages and Its Antioxidant Status Improved by Crab Shell Extract in Streptozotocin-induced Diabetic Rat

“…Chitin, chitosan, and their derivatives have important biological properties including antioxidant, anti-inflammatory, antitumor, antimicrobial, and antidiabetic effect, and their antioxidant effects play a vital role in nutrition and human health. [131415]…”

“…Various natural compounds with antidiabetic activity are used to control diabetes; our previous studies were shown inhibitory effects of crab shell extract (CSE) on breast cancer cell line (MCF7) and[20] improved pancreatic tissue changes caused by diabetes. [15] The aim of this study was to investigate the effect of CSE on structural changes and ferric-reducing antioxidant power (FRAP) level of renal tissue and urinary biochemical parameters in diabetic rat.…”