Criblage phytochimique et activité antimicrobienne de six rhizomes comestibles utilisés en médecine traditionnelle à Lubumbashi (RDC)

Bashige, V.; Bakari, A; Okusa, Philippe N.; Kalonda, E. M.; Lumbu, J. B. S.

doi:10.4314/ijbcs.v14i4.16

Cited by 12 publications

(12 citation statements)

References 14 publications

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“…The phytochemical screening was carried out using conventional reactions in solution in tubes, based on staining, precipitation, or the formation of foams. It consisted in looking for alkaloids, anthocyanins, coumarins, flavonoids, quinones, saponins, steroids, tannins and terpenoids for their antiplasmodial or antioxidant potential and cyanogenic heterosides for their toxic potential, following the protocols previously described [26][27][28].…”

Section: Identification Of Secondary Metabolitesmentioning

confidence: 99%

Phytochemical screening and antioxidant activity of methanolic extracts of 53 antimalarial plants from Bagira in Eastern DR Congo

Chiribagula¹,

Bakari²,

Ndjolo³

et al. 2020

GSC Biol. and Pharm. Sci.

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A previous study inventoried 53 plants used in traditional medicine in Bagira in Eastern Democratic Republic of Congo (DRC) in the management of malaria. During malaria disease, oxidative stress is responsible for the worsening of the patient's condition. This study aims to identify phytochemical groups and to evaluate antioxidant activity of 53 plants used in traditional medicine in Bagira to treat malaria. The phytochemical screening was carried out by conventional reactions in solution and antioxidant activity used in vitro method with 1,1-diphenyl-2-picrylhydrazyl radical (DPPH). Chemical screening has identified secondary metabolites with both antimalarial and antioxidant potential such as coumarins, steroids, saponins, tannins and terpenoids in more than 70% of plants. Antioxidant screening revealed for the first-time antioxidant activity of 18 plants, among which Dalbergia katangensis, Dialium angolense and Solanecio cydoniifolius with IC50 ≤ 1.6 µg / mL having the highest activities. This study shows that among plants used as antimalarial in Bagira several possess antioxidant power and contain many of groups presumed to be both antioxidant and antimalarial. This suggests that further studies continue to isolate compounds responsible for the proven activity.

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Section: Identification Of Secondary Metabolitesmentioning

confidence: 99%

Phytochemical screening and antioxidant activity of methanolic extracts of 53 antimalarial plants from Bagira in Eastern DR Congo

Chiribagula¹,

Bakari²,

Ndjolo³

et al. 2020

GSC Biol. and Pharm. Sci.

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show abstract

“…The plant extract was analyzed for the presence of some secondary metabolite including alkaloids, coumarins, flavonoids, saponins, steroids, tannins, terpenoids and phenols, using standard procedures in tube reaction [15,16].…”

Section: Phytochemical Screeningmentioning

confidence: 99%

Antiplasmodial, antioxidant and toxicological study of leaves extracts of Dalbergia katangensis Lecheneaud (Fabaceae) from Eastern DR Congo

Valentin¹,

Salvius²,

Joseph³

et al. 2020

GSC Adv. Res. Rev.

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Dalbergia katangensis is used in traditional medicine in Bukavu in management of malaria. In this study, antiplasmodial, antioxidant activities, and toxicological studies where carried out on aqueous and methanolic extracts of its leaves. The plant was selected following an ethnobotanical survey conducted in DR Congo and focusing on plants used traditionally to treat malaria. Extrac's phytochemical secondary metabolites were determined using standard procedures and the antiplasmodial activity was evaluated using 4-day suppressive test, while antioxidant activity was evaluated by DPPH assay. In acute toxicity, twenty animals (5/group) were given orally singular 2000 mg of extract/kg body weight (BW) then observed for 14 days. In sub-acute toxicity assay, 200, 400 mg/kg BW/Day were given orally, and animals were observed for 28 days. The total phenolic (0.58-0.61 mg GAEg -1 ), total flavonoid (0.14-0.15 mg QEg -1 ) and total terpenoids contents (0.32-0.55 %w/w) were in the same rate in the two extracts as well as the antioxidant activity with IC50 value 1.55 ± 0.012 and 1.71 ± 0.014 μg/mL. At the highest oral dose,400 mg/kg body weight, all extracts produced 73-75% chemo-suppression against P. berghei ANKA and 28 survival days. No deaths were recorded during the acute toxicity assay suggesting the LD50 >2000 mg/kg and no abnormal behavior or variation in toxicity biomarkers were observed during the subacute toxicity assessment. Dalbergia katangensis leaves extracts showed a great antiplasmodial and a very good antioxidant activity. It can be used to prepare antimalarial recipe or isolate antimalarial compounds in the future.

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Section: Phytochemical Screeningmentioning

confidence: 99%

In vivo antiplasmodial and toxicological studies of Dialium angolense Welw. Ex Oliv. (Fabaceae) leaves extracts, a medicinal plant from Eastern Congo

Valentin¹,

Salvius²,

Henry³

et al. 2020

World J. Bio. Pharm. Health Sci.

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Dialium angolense is used in traditional medicine in Bagira-Bukavu in the management of malaria. In this study, in vivo antiplasmodial and in vitro antioxidant activities, phytochemical screening of secondary metabolic and in vivo toxicological studies where carried out on aqueous and methanolic extracts of its leaves. The plant was selected following an ethnobotanical survey conducted in DR Congo and focusing on antimalarial plants. Extracts’ phytochemical secondary metabolites were determined using standard procedures and the antiplasmodial activity was evaluated using 4-day suppressive test, while antioxidant activity was evaluated by DPPH assay. In acute toxicity, eighteen animal (6/group) were given orally singular 2000 mg of extract/kg body weight (BW) then observed for 14 days. In sub-acute toxicity assay, 150 or 300 mg/kg BW/Day were given orally, and animals (6/group) were observed for 28 days. The total phenolic (0.89 - 0.98 mg GAEg-1), total flavonoid (0.42 - 0.44 mg QEg-1) and total tannin contents (0.080 – 0.098 mg GAEg-1) were in the same rate in the two extracts as well as the antioxidant activity with IC50 value 6.1 and 6.8 μg/mL. At the highest oral dose, 300 mg/kg body weight, all extracts produced 70.4–70.8% chemo-suppression against P. berghei ANKA and 28 survival days. No deaths were recorded during the acute toxicity assay suggesting the LD50 > 2000 mg/kg and no abnormal behavior or variation in toxicity biomarkers were observed during the subacute toxicity assessment. D. angolense leaves extracts showed a great antiplasmodial and a very good antioxidant activity. It can be used to prepare antimalarial recipe or isolate antimalarial compounds in the future.

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Criblage phytochimique et activité antimicrobienne de six rhizomes comestibles utilisés en médecine traditionnelle à Lubumbashi (RDC)

Cited by 12 publications

References 14 publications

Phytochemical screening and antioxidant activity of methanolic extracts of 53 antimalarial plants from Bagira in Eastern DR Congo

Phytochemical screening and antioxidant activity of methanolic extracts of 53 antimalarial plants from Bagira in Eastern DR Congo

Antiplasmodial, antioxidant and toxicological study of leaves extracts of Dalbergia katangensis Lecheneaud (Fabaceae) from Eastern DR Congo

In vivo antiplasmodial and toxicological studies of Dialium angolense Welw. Ex Oliv. (Fabaceae) leaves extracts, a medicinal plant from Eastern Congo

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