CRISPR-Cas13 Inhibitors Block RNA Editing in Bacteria and Mammalian Cells

Lin, Ping; Qin, Shugang; Pu, Qinqin; Wang, Zhihan; Wu, Qun; Gao, Pan; Schettler, Jacob; Guo, Kai; Li, Rongpeng; Li, Guoping; Huang, Canhua; Wei, Yuquan; Gao, George F.; Jiang, Jianxin; Wu, Min

doi:10.1016/j.molcel.2020.03.033

Cited by 73 publications

(53 citation statements)

References 61 publications

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“…It must be mentioned that using dead Cas13 is safer than Cas13 as it does not cut the RNA. Moreover, there are anti-CRISPR proteins, which can be used for deactivating the Cas13, whenever there are side effects [ [84] , [85] , [86] ]. Thus, if Cas13 can be programmed to efficiently target and cleave a wide variety of mammalian viruses’ RNA genome especially SARS-CoV-2 genome by investigating the prevalence of target sites in ssRNA viral genomes, it has the potential as an antiviral therapy, which has fewer off-targets.…”

Section: Crispr/cas13mentioning

confidence: 99%

CRISPR/Cas13: A potential therapeutic option of COVID-19

Lotfi

Rezaei

2020

Biomedicine & Pharmacotherapy

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Section: Crispr/cas13mentioning

confidence: 99%

CRISPR/Cas13: A potential therapeutic option of COVID-19

Lotfi

Rezaei

2020

Biomedicine & Pharmacotherapy

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“…The world’s largest canola importer, EU, has imposed strict regulations on GM organisms, which affects technological choices in improving canola. Taking environmental and legislation perspectives, gene editing tools, such as CRISPR/Cas, are the most promising technology for enhancement of canola for commercial purpose [ 178 ]. With the advance of genomics and pan-genomics, the genetic architecture underlying response to the major pathogens and abiotic stresses such as salinity and drought need to be further dissected.…”

Section: Prospects and Future Directionsmentioning

confidence: 99%

The Use of Genetic and Gene Technologies in Shaping Modern Rapeseed Cultivars (Brassica napus L.)

Ton

Neik

Batley

2020

Genes

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Since their domestication, Brassica oilseed species have undergone progressive transformation allied with the development of breeding and molecular technologies. The canola (Brassica napus) crop has rapidly expanded globally in the last 30 years with intensive innovations in canola varieties, providing for a wider range of markets apart from the food industry. The breeding efforts of B. napus, the main source of canola oil and canola meal, have been mainly focused on improving seed yield, oil quality, and meal quality along with disease resistance, abiotic stress tolerance, and herbicide resistance. The revolution in genetics and gene technologies, including genetic mapping, molecular markers, genomic tools, and gene technology, especially gene editing tools, has allowed an understanding of the complex genetic makeup and gene functions in the major bioprocesses of the Brassicales, especially Brassica oil crops. Here, we provide an overview on the contributions of these technologies in improving the major traits of B. napus and discuss their potential use to accomplish new improvement targets.

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“…The latest AcrI protein, AcrIB1, was found recently in Leptotrichia buccalis DSM 1135 strains (Lin et al, 2020). The way it exerts its action, however, was not clarified yet.…”

Section: Type I Anti-crispr Proteins (Acri): Discovery and Working Mementioning

confidence: 99%

Types I and V Anti-CRISPR Proteins: From Phage Defense to Eukaryotic Synthetic Gene Circuits

Marchisio

2020

Front. Bioeng. Biotechnol.

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Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas (CRISPRassociated proteins), a prokaryotic RNA-mediated adaptive immune system, has been repurposed for gene editing and synthetic gene circuit construction both in bacterial and eukaryotic cells. In the last years, the emergence of the anti-CRISPR proteins (Acrs), which are natural OFF-switches for CRISPR-Cas, has provided a new means to control CRISPR-Cas activity and promoted a further development of CRISPR-Cas-based biotechnological toolkits. In this review, we focus on type I and type V-A anti-CRISPR proteins. We first narrate Acrs discovery and analyze their inhibitory mechanisms from a structural perspective. Then, we describe their applications in gene editing and transcription regulation. Finally, we discuss the potential future usageand corresponding possible challenges-of these two kinds of anti-CRISPR proteins in eukaryotic synthetic gene circuits.

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CRISPR-Cas13 Inhibitors Block RNA Editing in Bacteria and Mammalian Cells

Cited by 73 publications

References 61 publications

CRISPR/Cas13: A potential therapeutic option of COVID-19

CRISPR/Cas13: A potential therapeutic option of COVID-19

The Use of Genetic and Gene Technologies in Shaping Modern Rapeseed Cultivars (Brassica napus L.)

Types I and V Anti-CRISPR Proteins: From Phage Defense to Eukaryotic Synthetic Gene Circuits

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