Critical review: Injectability of calcium phosphate pastes and cements

O'Neill, R.E.; McCarthy, Helen; Montúfar, Edgar B.; Ginebra, Maria‐Pau; Wilson, D.I.; Lennon, Alex; Dunne, Nicholas

doi:10.1016/j.actbio.2016.11.019

Cited by 207 publications

(201 citation statements)

References 158 publications

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“…The calcination of fish bones results in a single-phase (hydroxyapatite [HA]) or bi-phasic (hydroxyapatite-tricalcium phosphate) bioceramics depending on the processing conditions as well as the content of the fish bones. 9,10 The major problem of CPC is the low bending strength and its fracture toughness. The heat-treated fish bone has a porous structure and allows faster bone in-growth due to its excellent biocompatible and osteoconductive properties.…”

Section: Introductionmentioning

confidence: 99%

In vitro apatite formation of calcium phosphate composite synthesized from fish bone

Asadollahzadeh

Rabiee

Salimi-Kenari

2019

Int J Applied Ceramic Tech

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Calcium phosphate‐based composite (CPC) is the main biomaterial substitute used for bone repair. Properties affecting bioactivity of this composite vary depending on the types of calcium phosphate crystalline phases. Hence, in this study, bioactivity behavior of novel CPC cement by the incorporation of calcium phosphate (CP), which was obtained from fish bones, dicalcium phosphate dehydrate, and chitosan solution, was monitored in simulated body fluid (SBF). In advance, the microstructure of CP produced by heat treatment (annealing) of fish bone was evaluated at two different temperatures 600 and 900°C. The X‐ray diffraction (XRD) results showed that there was no secondary phase formation aside from natural hydroxyapatite (HA) in bones annealed; and the annealing process enhanced the crystallinity of CP phase in the bone matrix particularly when annealed at 900°C. After incubation of CPC cement in SBF, bone bonding ability and producing of biomimetic HA coat on the CPC cement surface were confirmed using XRD, fourier‐transform infrared spectroscopy, and scanning electron microscopy. The analysis results show that needle‐like and cauliflower apatite layer with the crystallite size about 100 nm was grown on the surface of CPC cement after 28 days incubation in SBF. Regardless of above findings, we conclude that varying the annealing temperature has tremendous effect on the production of natural HA from fish bone with required properties and the ultimate morphology of obtained CPC cements after soaking is directly depended on the degree of crystallinity of the prepared natural HA.

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Section: Introductionmentioning

confidence: 99%

In vitro apatite formation of calcium phosphate composite synthesized from fish bone

Asadollahzadeh

Rabiee

Salimi-Kenari

2019

Int J Applied Ceramic Tech

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“…Degradability of CDHA is almost identical to that of beta‐tricalcium phosphate, but it showed to have a better cell seeding efficacy, possibly due to its higher specific surface area . Technical advances in the technique of rapid prototyping (RP) 3D plotting now allow low‐temperature manufacturing of CDHA scaffolds . These scaffolds could be adapted to the shape of any given structural defect of bone.…”

Section: Introductionmentioning

confidence: 99%

Effect of bone sialoprotein coated three‐dimensional printed calcium phosphate scaffolds on primary human osteoblasts

et al. 2018

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The combination of the two techniques of rapid prototyping 3D-plotting and bioactive surface functionalization is presented, with emphasis on the in vitro effect of Bone Sialoprotein (BSP) on primary human osteoblasts (hOBs). Our primary objective was to demonstrate the BSP influence on the expression of distinctive osteoblast markers in hOBs. Secondary objectives included examinations of the scaffolds' surface and the stability of BSP-coating as well as investigations of cell viability and proliferation. 3D-plotted calcium phosphate cement (CPC) scaffolds were coated with BSP via physisorption. hOBs were seeded on the coated scaffolds, followed by cell viability measurements, gene expression analysis and visualization. Physisorption is an effective method for BSP-coating. Coating with higher BSP concentrations leads to enhanced BSP release. Two BSP concentrations (50 and 200 μg/mL) were examined in this study. The lower BSP concentration (50 µg/mL) decreased ALP and SPARC expression, whereas the higher BSP concentration (200 μg/mL) did not change gene marker expression. Enhanced cell viability was observed on BSP-coated scaffolds on day 3. hOBs developed a polygonal shape and connected in an intercellular network under BSP influence. Quantitative cell morphology analyses demonstrated for BSP-coated CPCs an enhanced cell area and reduced circularity. The strength of the above-mentioned effects of BSP-coated scaffolds in vivo is unknown, and future work is focusing on bone ingrowth and vascularization in vivo. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2565-2575, 2018.

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“…Calcium phosphate cements (CPC) are of high clinical interest for bone replacement due to their well-known biocompatibility in vivo [1–3]. Although a diversity of formulations are possible there are only two main products of the cement dissolution–precipitation reaction.…”

Section: Introductionmentioning

confidence: 99%

In vitro ion adsorption and cytocompatibility of dicalcium phosphate ceramics

et al. 2017

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Background In vitro cell testing of degradable bioceramics such as brushite or monetite is often challenging due to the ion release into or adsorption from the culture medium. These ionic changes are then mostly responsible for cell proliferation and activity, which prohibits the investigation of effects originating from surface topography or further material modifications.MethodsHere, we aimed to solve this problem by developing a pre-conditioning regime following the repeated immersion of brushite and monetite samples in various Ca2+, Mg2+ and PO4 3− containing electrolytes, followed by studying ion adsorption / release as well as changes in phase composition and in vitro cytocompatibility with MG63 cells.ResultsThe results demonstrated that by using DMEM cell culture medium in a ratio of 10 ml/sample was sufficient to minimize changes of ionic composition after 7 d with a daily change of the medium. This leads to changes of the surface composition with dissolution of the brushite phase. In turn, this also positively influences the in vitro cytocompatibility with a 2–3 fold higher cell number and cell activity on the DMEM pretreated surfaces.ConclusionsControlled sample washing prior to cell testing using DMEM medium seems to be a valuable procedure not only to stabilize the pH during cell culture but also to maintain ion concentrations within a cell friendly range.

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Critical review: Injectability of calcium phosphate pastes and cements

Cited by 207 publications

References 158 publications

In vitro apatite formation of calcium phosphate composite synthesized from fish bone

In vitro apatite formation of calcium phosphate composite synthesized from fish bone

Effect of bone sialoprotein coated three‐dimensional printed calcium phosphate scaffolds on primary human osteoblasts

In vitro ion adsorption and cytocompatibility of dicalcium phosphate ceramics

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